Characterization of vesicles prepared with various non-ionic surfactants mixed with cholesterol

• Main Authors: Manosroi A., Wongtrakul P., Manosroi J., Sakai H., Sugawara F., Yuasa M., Abe M.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-0038022715&partnerID=40&md5=737a049b505de6d5e4bea25dc4a71fbc; http://cmuir.cmu.ac.th/handle/6653943832/4564
• Institution: Chiang Mai University

The vesicles (niosomes) prepared with hydrated mixture of various non-ionic surfactants and cholesterol were studied. The bilayer formation was characterized by X-cross formation under light polarization microscope and the ability of the vesicles to entrap water-soluble substance. Membrane rigidity was measured by means of mobility of fluorescence probe as a function of temperatures. The entrapment efficiencies of the vesicles and microviscosities of the vesicular membrane depended on alkyl chain length of non-ionic surfactants and amount of cholesterol used to prepare vesicles. The stearyl chain (C18) non-ionic surfactant vesicles showed higher entrapment efficiency than the lauryl chain (C12) non-ionic surfactant vesicles. Cholesterol was used to complete the hydrophobic moiety of single alkyl chain non-ionic surfactants for vesicle formation. Niosome prepared with Tween 61 bearing a long alkyl chain and a large hydrophilic moiety in the combination with cholesterol at 1:1 molar ratio was found to have the highest entrapment efficiency of water soluble substances. © 2003 Elsevier Science B.V. All rights reserved.