Analgesic, Anti-Inflammatory, and Chondroprotective Activities of Cryptolepis buchanani Extract: In Vitro and in Vivo Studies

© 2014 Nutthiya Hanprasertpong et al. Cryptolepis buchanani Roem. & Schult. is widely used in folk medicine in Southeast Asia for treating muscle tension and arthritis. This study aimed to investigate an analgesic activity of the methanol extract of C. buchanani (CBE) in acetic acid-induced wr...

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Main Authors: Nutthiya Hanprasertpong, Supanimit Teekachunhatean, Rujirek Chaiwongsa, Siriwan Ongchai, Puongtip Kunanusorn, Chaichan Sangdee, Ampai Panthong, Samreang Bunteang, Narong Nathasaen, Vichai Reutrakul
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84930883374&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/45707
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-457072018-01-24T06:15:24Z Analgesic, Anti-Inflammatory, and Chondroprotective Activities of Cryptolepis buchanani Extract: In Vitro and in Vivo Studies Nutthiya Hanprasertpong Supanimit Teekachunhatean Rujirek Chaiwongsa Siriwan Ongchai Puongtip Kunanusorn Chaichan Sangdee Ampai Panthong Samreang Bunteang Narong Nathasaen Vichai Reutrakul © 2014 Nutthiya Hanprasertpong et al. Cryptolepis buchanani Roem. & Schult. is widely used in folk medicine in Southeast Asia for treating muscle tension and arthritis. This study aimed to investigate an analgesic activity of the methanol extract of C. buchanani (CBE) in acetic acid-induced writhing response in mice, and to examine its anti-inflammatory activity in ethyl phenylpropiolate- (EPP-) induced ear edema and carrageenan-induced paw edema in rats. Its effects on cartilage degradation induced by interleukin-1β (IL-1β) in porcine cartilage explant culture were also determined. This study demonstrated that CBE significantly reduced acetic acid-induced writhing response. It also inhibited edema formation in both EPP-induced ear edema and carrageenan-induced paw edema models. In cartilage explant culture, CBE significantly reduced the sulfated glycosaminoglycan and hyaluronan released into culture media while it reserved the uronic acid and collagen within the cartilage tissues. It also suppressed the matrix metalloproteinase-2 activity with no effect on cell viability. In conclusion, CBE shows analgesic, anti-inflammatory, and chondroprotective effects in this preliminary study. Therefore, CBE may be useful as an alternative treatment for osteoarthritis. 2018-01-24T06:15:24Z 2018-01-24T06:15:24Z 2014-01-01 Journal 23146141 23146133 2-s2.0-84930883374 10.1155/2014/978582 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84930883374&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/45707
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description © 2014 Nutthiya Hanprasertpong et al. Cryptolepis buchanani Roem. & Schult. is widely used in folk medicine in Southeast Asia for treating muscle tension and arthritis. This study aimed to investigate an analgesic activity of the methanol extract of C. buchanani (CBE) in acetic acid-induced writhing response in mice, and to examine its anti-inflammatory activity in ethyl phenylpropiolate- (EPP-) induced ear edema and carrageenan-induced paw edema in rats. Its effects on cartilage degradation induced by interleukin-1β (IL-1β) in porcine cartilage explant culture were also determined. This study demonstrated that CBE significantly reduced acetic acid-induced writhing response. It also inhibited edema formation in both EPP-induced ear edema and carrageenan-induced paw edema models. In cartilage explant culture, CBE significantly reduced the sulfated glycosaminoglycan and hyaluronan released into culture media while it reserved the uronic acid and collagen within the cartilage tissues. It also suppressed the matrix metalloproteinase-2 activity with no effect on cell viability. In conclusion, CBE shows analgesic, anti-inflammatory, and chondroprotective effects in this preliminary study. Therefore, CBE may be useful as an alternative treatment for osteoarthritis.
format Journal
author Nutthiya Hanprasertpong
Supanimit Teekachunhatean
Rujirek Chaiwongsa
Siriwan Ongchai
Puongtip Kunanusorn
Chaichan Sangdee
Ampai Panthong
Samreang Bunteang
Narong Nathasaen
Vichai Reutrakul
spellingShingle Nutthiya Hanprasertpong
Supanimit Teekachunhatean
Rujirek Chaiwongsa
Siriwan Ongchai
Puongtip Kunanusorn
Chaichan Sangdee
Ampai Panthong
Samreang Bunteang
Narong Nathasaen
Vichai Reutrakul
Analgesic, Anti-Inflammatory, and Chondroprotective Activities of Cryptolepis buchanani Extract: In Vitro and in Vivo Studies
author_facet Nutthiya Hanprasertpong
Supanimit Teekachunhatean
Rujirek Chaiwongsa
Siriwan Ongchai
Puongtip Kunanusorn
Chaichan Sangdee
Ampai Panthong
Samreang Bunteang
Narong Nathasaen
Vichai Reutrakul
author_sort Nutthiya Hanprasertpong
title Analgesic, Anti-Inflammatory, and Chondroprotective Activities of Cryptolepis buchanani Extract: In Vitro and in Vivo Studies
title_short Analgesic, Anti-Inflammatory, and Chondroprotective Activities of Cryptolepis buchanani Extract: In Vitro and in Vivo Studies
title_full Analgesic, Anti-Inflammatory, and Chondroprotective Activities of Cryptolepis buchanani Extract: In Vitro and in Vivo Studies
title_fullStr Analgesic, Anti-Inflammatory, and Chondroprotective Activities of Cryptolepis buchanani Extract: In Vitro and in Vivo Studies
title_full_unstemmed Analgesic, Anti-Inflammatory, and Chondroprotective Activities of Cryptolepis buchanani Extract: In Vitro and in Vivo Studies
title_sort analgesic, anti-inflammatory, and chondroprotective activities of cryptolepis buchanani extract: in vitro and in vivo studies
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84930883374&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/45707
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