Effect of Swiss-type heterocellular HPFH from XmnI-<sup>G</sup>γ and HBBP1 polymorphisms on HbF, HbE, MCV and MCH levels in Thai HbE carriers

Relationships of Swiss-type heterocellular HPFH as functions of XmnI- G γ and HBBP1:rs2071348 polymorphisms and HbF, HbE, MCV and MCH in HbE carriers were evaluated in 52 non-anemic and α-thalassemia-free Thai HbE carriers. HbF and HbE levels were measured using cation-exchange HPLC. MCV and MCH wer...

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Bibliographic Details
Main Authors: Sasiwan Kerdpoo, Ektong Limweeraprajak, Thanusak Tatu
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84899428147&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/45710
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Institution: Chiang Mai University
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Summary:Relationships of Swiss-type heterocellular HPFH as functions of XmnI- G γ and HBBP1:rs2071348 polymorphisms and HbF, HbE, MCV and MCH in HbE carriers were evaluated in 52 non-anemic and α-thalassemia-free Thai HbE carriers. HbF and HbE levels were measured using cation-exchange HPLC. MCV and MCH were determined using an automated blood counter. The XmnI- G γ polymorphism was identified by XmnI digestion of amplified products, and the HBBP1:rs2071348 polymorphism by tetra-ARMS-PCR. HbF levels in HbE carriers were higher than those in normal individuals. HbF levels > 0.8 % indicated the Swiss-type heterocellular HPFH in these subjects, rendering a prevalence of 40.4 %. The XmnI- G γ (+) and HBBP1:rs2071348 (C) alleles were modestly positively correlated with elevated HbF, elevated MCH and lowered HbE values. This study thus confirms the influence of the XmnI- G γ and HBBP1:rs2071348 polymorphisms on HbF production. The present study demonstrates the association of XmnI- G γ and HBBP1:rs2071348 with HbF, HbE, MCV and MCH in HbE carriers for the first time, and highlights the effect of elevated HbF production on HbE levels. © 2014 The Japanese Society of Hematology.