Cellular uptake enhancement of Tat-GFP fusion protein loaded in elastic niosomes

• Main Authors: Manosroi A., Lohcharoenkal W., Gotz F., Werner R.G., Manosroi W., Manosroi J.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.ncbi.nlm.nih.gov/pubmed/3502482; http://www.scopus.com/inward/record.url?eid=2-s2.0-80052326914&partnerID=40&md5=cadd1239ba571b5b16552ecc12fe80a1; http://cmuir.cmu.ac.th/handle/6653943832/4581
• Institution: Chiang Mai University

This study has demonstrated the enhancement of cellular uptake of GFP when fused with Tat (Tat- GFP) and loaded in elastic niosomes. GFP and the GFP fused with Tat at C- and N-terminals were expressed in E. coli BL21 (DE3). The N-terminal Tat-GFP fusion protein (Tat-GFP) which showed the highest upt...