Enhancement of 17alpha-hydroxyprogesterone production from progesterone by biotransformation using hydroxypropyl-beta-cyclodextrin complexation technique

An enhancement of 17alpha-hydroxyprogesterone (17alpha-HP) production from progesterone by biotransformation using hydroxypropyl-beta-cyclodextrin (HPbetaCD) complexation together with aeration and sonication technique was demonstrated. The progesterone-hydroxypropyl-beta-cyclodextrin complex was pr...

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Main Authors: Manosroi J., Saowakhon S., Manosroi A.
Format: Article
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/4584
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-45842014-08-30T02:42:37Z Enhancement of 17alpha-hydroxyprogesterone production from progesterone by biotransformation using hydroxypropyl-beta-cyclodextrin complexation technique Manosroi J. Saowakhon S. Manosroi A. An enhancement of 17alpha-hydroxyprogesterone (17alpha-HP) production from progesterone by biotransformation using hydroxypropyl-beta-cyclodextrin (HPbetaCD) complexation together with aeration and sonication technique was demonstrated. The progesterone-hydroxypropyl-beta-cyclodextrin complex was prepared by co-evaporation method. The percentage yield of 17alpha-HP from P of 11.26+/-0.64% at 24h was observed in Curvularia lunata ATCC 12017. In the complex form of P, together with sonication at 40kHz for 5s and aeration, the yield of 17alpha-HP was increased to 72.92+/-4.28% which was about 6.5 and 1.3 times of that from the uncomplexed (P) and the complexed (PC), respectively without sonication and aeration. The increased aqueous solubility of P by complexation with HPbetaCD was the main factor which increased the yield of 17alpha-HP, while aeration had more effect on P than PC. Sonication did not significantly increased the yield of the product from both P and PC. When both aeration and sonication were used in the PC system, the product yield was increased significantly more than that from P. The result from this study can be applied for the biotransformation of other poor aqueous soluble precursors. 2014-08-30T02:42:37Z 2014-08-30T02:42:37Z 2008 Journal Article 0960-0760 18983919 http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/4584 eng
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description An enhancement of 17alpha-hydroxyprogesterone (17alpha-HP) production from progesterone by biotransformation using hydroxypropyl-beta-cyclodextrin (HPbetaCD) complexation together with aeration and sonication technique was demonstrated. The progesterone-hydroxypropyl-beta-cyclodextrin complex was prepared by co-evaporation method. The percentage yield of 17alpha-HP from P of 11.26+/-0.64% at 24h was observed in Curvularia lunata ATCC 12017. In the complex form of P, together with sonication at 40kHz for 5s and aeration, the yield of 17alpha-HP was increased to 72.92+/-4.28% which was about 6.5 and 1.3 times of that from the uncomplexed (P) and the complexed (PC), respectively without sonication and aeration. The increased aqueous solubility of P by complexation with HPbetaCD was the main factor which increased the yield of 17alpha-HP, while aeration had more effect on P than PC. Sonication did not significantly increased the yield of the product from both P and PC. When both aeration and sonication were used in the PC system, the product yield was increased significantly more than that from P. The result from this study can be applied for the biotransformation of other poor aqueous soluble precursors.
format Article
author Manosroi J.
Saowakhon S.
Manosroi A.
spellingShingle Manosroi J.
Saowakhon S.
Manosroi A.
Enhancement of 17alpha-hydroxyprogesterone production from progesterone by biotransformation using hydroxypropyl-beta-cyclodextrin complexation technique
author_facet Manosroi J.
Saowakhon S.
Manosroi A.
author_sort Manosroi J.
title Enhancement of 17alpha-hydroxyprogesterone production from progesterone by biotransformation using hydroxypropyl-beta-cyclodextrin complexation technique
title_short Enhancement of 17alpha-hydroxyprogesterone production from progesterone by biotransformation using hydroxypropyl-beta-cyclodextrin complexation technique
title_full Enhancement of 17alpha-hydroxyprogesterone production from progesterone by biotransformation using hydroxypropyl-beta-cyclodextrin complexation technique
title_fullStr Enhancement of 17alpha-hydroxyprogesterone production from progesterone by biotransformation using hydroxypropyl-beta-cyclodextrin complexation technique
title_full_unstemmed Enhancement of 17alpha-hydroxyprogesterone production from progesterone by biotransformation using hydroxypropyl-beta-cyclodextrin complexation technique
title_sort enhancement of 17alpha-hydroxyprogesterone production from progesterone by biotransformation using hydroxypropyl-beta-cyclodextrin complexation technique
publishDate 2014
url http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/4584
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