Biotransformation of cortexolone to hydrocortisone by molds using a rapid color development assay
Capabilities of 22 molds were assessed for 11beta-hydroxylation of cortexolone (Reichstein's compound S) to hydrocortisone. The biotransformation capability was compared for solid-state and submerged monocultures of the molds under otherwise identical conditions. A novel rapid color development...
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Format: | Comparative Study |
Language: | English |
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2014
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Online Access: | http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/4586 |
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th-cmuir.6653943832-45862014-08-30T02:42:37Z Biotransformation of cortexolone to hydrocortisone by molds using a rapid color development assay Manosroi J. Chisti Y. Manosroi A. Capabilities of 22 molds were assessed for 11beta-hydroxylation of cortexolone (Reichstein's compound S) to hydrocortisone. The biotransformation capability was compared for solid-state and submerged monocultures of the molds under otherwise identical conditions. A novel rapid color development assay and thin layer chromatography were used to qualitatively establish the ability of the fungi to convert cortexolone to hydrocortisone. These assays were validated and supplemented with data from high performance liquid chromatography to obtain quantitative information on the biotransformation. Nearly all the fungi consumed a significant fraction of the cortexolone fed, but only four (i.e. two isolates of Cunninghamella blakesleeana, C. echinulata and Curvularia lunata) yielded measurable quantities of hydrocortisone. Submerged cultures generally gave significantly greater yield of hydrocortisone compared to equivalent solid-state cultures. 2014-08-30T02:42:37Z 2014-08-30T02:42:37Z Comparative Study 0555-1099 17066954 http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/4586 eng |
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Capabilities of 22 molds were assessed for 11beta-hydroxylation of cortexolone (Reichstein's compound S) to hydrocortisone. The biotransformation capability was compared for solid-state and submerged monocultures of the molds under otherwise identical conditions. A novel rapid color development assay and thin layer chromatography were used to qualitatively establish the ability of the fungi to convert cortexolone to hydrocortisone. These assays were validated and supplemented with data from high performance liquid chromatography to obtain quantitative information on the biotransformation. Nearly all the fungi consumed a significant fraction of the cortexolone fed, but only four (i.e. two isolates of Cunninghamella blakesleeana, C. echinulata and Curvularia lunata) yielded measurable quantities of hydrocortisone. Submerged cultures generally gave significantly greater yield of hydrocortisone compared to equivalent solid-state cultures. |
format |
Comparative Study |
author |
Manosroi J. Chisti Y. Manosroi A. |
spellingShingle |
Manosroi J. Chisti Y. Manosroi A. Biotransformation of cortexolone to hydrocortisone by molds using a rapid color development assay |
author_facet |
Manosroi J. Chisti Y. Manosroi A. |
author_sort |
Manosroi J. |
title |
Biotransformation of cortexolone to hydrocortisone by molds using a rapid color development assay |
title_short |
Biotransformation of cortexolone to hydrocortisone by molds using a rapid color development assay |
title_full |
Biotransformation of cortexolone to hydrocortisone by molds using a rapid color development assay |
title_fullStr |
Biotransformation of cortexolone to hydrocortisone by molds using a rapid color development assay |
title_full_unstemmed |
Biotransformation of cortexolone to hydrocortisone by molds using a rapid color development assay |
title_sort |
biotransformation of cortexolone to hydrocortisone by molds using a rapid color development assay |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/4586 |
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1681420265095954432 |