Isolation and in vitro antimalarial activity of hexane extract from Thai Picrasma javanica B1 stembark

The in vitro antimalarial activities against Plasmodium falciparum K1 of four extracts from the stembark of Picrasma javanica B1; ie water, methanol, chloroform and hexane extracts were studied using a modification of the [3H]hypoxanthine incorporation method. It was found that the hexane extract sh...

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Main Authors: Saiin C., Rattanajak R., Kamchonwongpaisan S., Ingkaninan K., Sukontason K., Baramee A., Sirithunyalug B.
Format: In Vitro
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/4590
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-45902014-08-30T02:42:38Z Isolation and in vitro antimalarial activity of hexane extract from Thai Picrasma javanica B1 stembark Saiin C. Rattanajak R. Kamchonwongpaisan S. Ingkaninan K. Sukontason K. Baramee A. Sirithunyalug B. The in vitro antimalarial activities against Plasmodium falciparum K1 of four extracts from the stembark of Picrasma javanica B1; ie water, methanol, chloroform and hexane extracts were studied using a modification of the [3H]hypoxanthine incorporation method. It was found that the hexane extract showed in vitro antimalarial activity with IC50 of 3.3 microg/ml. The extract was further fractionated using quick column chromatography, resulting in ten fractions. Fraction V was the most effective against P. falciparum K1 with IC50 of 4.4 microg/ml. Further isolation of fraction V using a column chromatographic technique provided six fractions. According to 1H- and 13C-NMR spectra, it could be concluded that the major compound in fraction V-3 was beta-sitosterol. Unfortunately, the antimalarial activity of beta-sitosterol could not be determined because of its low solubility in DMSO. However, fractions V-2 and V-4 still showed in vitro antimalarial activities with IC50 of 2.8 and 3.4 microg/ml, respectively. The further fractionation of these two active fractions could lead to promising candidates as antimalarial agents. 2014-08-30T02:42:38Z 2014-08-30T02:42:38Z 2003 In Vitro 0125-1562 19230571 http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/4590 eng
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description The in vitro antimalarial activities against Plasmodium falciparum K1 of four extracts from the stembark of Picrasma javanica B1; ie water, methanol, chloroform and hexane extracts were studied using a modification of the [3H]hypoxanthine incorporation method. It was found that the hexane extract showed in vitro antimalarial activity with IC50 of 3.3 microg/ml. The extract was further fractionated using quick column chromatography, resulting in ten fractions. Fraction V was the most effective against P. falciparum K1 with IC50 of 4.4 microg/ml. Further isolation of fraction V using a column chromatographic technique provided six fractions. According to 1H- and 13C-NMR spectra, it could be concluded that the major compound in fraction V-3 was beta-sitosterol. Unfortunately, the antimalarial activity of beta-sitosterol could not be determined because of its low solubility in DMSO. However, fractions V-2 and V-4 still showed in vitro antimalarial activities with IC50 of 2.8 and 3.4 microg/ml, respectively. The further fractionation of these two active fractions could lead to promising candidates as antimalarial agents.
format In Vitro
author Saiin C.
Rattanajak R.
Kamchonwongpaisan S.
Ingkaninan K.
Sukontason K.
Baramee A.
Sirithunyalug B.
spellingShingle Saiin C.
Rattanajak R.
Kamchonwongpaisan S.
Ingkaninan K.
Sukontason K.
Baramee A.
Sirithunyalug B.
Isolation and in vitro antimalarial activity of hexane extract from Thai Picrasma javanica B1 stembark
author_facet Saiin C.
Rattanajak R.
Kamchonwongpaisan S.
Ingkaninan K.
Sukontason K.
Baramee A.
Sirithunyalug B.
author_sort Saiin C.
title Isolation and in vitro antimalarial activity of hexane extract from Thai Picrasma javanica B1 stembark
title_short Isolation and in vitro antimalarial activity of hexane extract from Thai Picrasma javanica B1 stembark
title_full Isolation and in vitro antimalarial activity of hexane extract from Thai Picrasma javanica B1 stembark
title_fullStr Isolation and in vitro antimalarial activity of hexane extract from Thai Picrasma javanica B1 stembark
title_full_unstemmed Isolation and in vitro antimalarial activity of hexane extract from Thai Picrasma javanica B1 stembark
title_sort isolation and in vitro antimalarial activity of hexane extract from thai picrasma javanica b1 stembark
publishDate 2014
url http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/4590
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