Effect of histamine and antihistamines on interleukin-1 production by human monocytes

This study was carried out on the effect of histamine hydrochloride and its antagonists on the production of interleukin-1 (IL-1) by lipopolysaccharide (LPS)-stimulated adherent human monocytes (AHM) from normal healthy blood donors. IL-1 activity was evaluated by incorporation of [3H]-thymidine in...

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Main Authors: Manosroi J., Manosroi A., Vithayasai V.
Format: In Vitro
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/4596
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-45962014-08-30T02:42:38Z Effect of histamine and antihistamines on interleukin-1 production by human monocytes Manosroi J. Manosroi A. Vithayasai V. This study was carried out on the effect of histamine hydrochloride and its antagonists on the production of interleukin-1 (IL-1) by lipopolysaccharide (LPS)-stimulated adherent human monocytes (AHM) from normal healthy blood donors. IL-1 activity was evaluated by incorporation of [3H]-thymidine in mouse thymocytes in samples of 1:3 dilution. The result indicated that histamine hydrochloride significantly suppressed IL-1 production by AHM at 10(-3) M and 10(-10) M in 14 donors with maximal suppression observed at 10(-3) M. A 1-hr incubation with histamine hydrochloride (10(-3) M) before addition of LPS was found to be appropriate. Cimetidine, an H2-antagonist at 10(-3) M, 10(-5) M, and 10(-7) M significantly inhibited the effect of histamine hydrochloride (10(-3) M) and gave maximum inhibition at 10(-5) M, whereas chlorpheniramine maleate, and H1-antagonist had no significant inhibitory effect at the concentrations studied (10(-4) M, 10(-5) M, and 10(-7) M). Histamine hydrochloride (10(-3) M) added alone had no significant suppressive effect, while cimetidine (10(-5) M) alone had a significant stimulatory effect on IL-1 production by AHM. 2014-08-30T02:42:38Z 2014-08-30T02:42:38Z 1987 In Vitro 0385-5600 3502482 http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/4596 eng
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description This study was carried out on the effect of histamine hydrochloride and its antagonists on the production of interleukin-1 (IL-1) by lipopolysaccharide (LPS)-stimulated adherent human monocytes (AHM) from normal healthy blood donors. IL-1 activity was evaluated by incorporation of [3H]-thymidine in mouse thymocytes in samples of 1:3 dilution. The result indicated that histamine hydrochloride significantly suppressed IL-1 production by AHM at 10(-3) M and 10(-10) M in 14 donors with maximal suppression observed at 10(-3) M. A 1-hr incubation with histamine hydrochloride (10(-3) M) before addition of LPS was found to be appropriate. Cimetidine, an H2-antagonist at 10(-3) M, 10(-5) M, and 10(-7) M significantly inhibited the effect of histamine hydrochloride (10(-3) M) and gave maximum inhibition at 10(-5) M, whereas chlorpheniramine maleate, and H1-antagonist had no significant inhibitory effect at the concentrations studied (10(-4) M, 10(-5) M, and 10(-7) M). Histamine hydrochloride (10(-3) M) added alone had no significant suppressive effect, while cimetidine (10(-5) M) alone had a significant stimulatory effect on IL-1 production by AHM.
format In Vitro
author Manosroi J.
Manosroi A.
Vithayasai V.
spellingShingle Manosroi J.
Manosroi A.
Vithayasai V.
Effect of histamine and antihistamines on interleukin-1 production by human monocytes
author_facet Manosroi J.
Manosroi A.
Vithayasai V.
author_sort Manosroi J.
title Effect of histamine and antihistamines on interleukin-1 production by human monocytes
title_short Effect of histamine and antihistamines on interleukin-1 production by human monocytes
title_full Effect of histamine and antihistamines on interleukin-1 production by human monocytes
title_fullStr Effect of histamine and antihistamines on interleukin-1 production by human monocytes
title_full_unstemmed Effect of histamine and antihistamines on interleukin-1 production by human monocytes
title_sort effect of histamine and antihistamines on interleukin-1 production by human monocytes
publishDate 2014
url http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/4596
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