ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA
Plants in family Apocynaceae, such as Plumeria sp., Adenium sp., Alstonia sp., Catharanthus sp. and Allamanda sp. are very famous as a household trees. The dominant feature of these plants were white latex which were in all parts of the trees but they have different features depending on their part...
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เชียงใหม่ : บัณฑิตวิทยาลัย มหาวิทยาลัยเชียงใหม่
2018
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th-cmuir.6653943832-460792018-04-10T20:08:25Z ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA DNA Fingerprinting of Some Plants in Family Apocynaceae by Randomly Amplified Polymorphic DNA Technique เจนใจ สุธีพรวิโรจน์ สิริพร โรจน์อารยานนท์ ลายพิมพ์ ดีเอ็นเอ ลั่นทม Plants in family Apocynaceae, such as Plumeria sp., Adenium sp., Alstonia sp., Catharanthus sp. and Allamanda sp. are very famous as a household trees. The dominant feature of these plants were white latex which were in all parts of the trees but they have different features depending on their parts such as size, flower and leave. DNA relationship among some plants in family Apocynaceae was studied by RAPD technique. The efficiency of forty primers experimental found that the proper primer for making DNA fingerprint was nine primers which were O-01, O-18, OPB-07, OPB-08, OPD-20, OPW-09, R-37, R-55 and R-105. They produce 276 polymorphic DNA bands. The analysis of DNA fingerprint data was found that the relationship of DNA by using 3.2 phylic program and Tree Veiw 1.6.6. The result could be grouped by unweighted pair group method with arithmetic average (UPGMA) in five groups consisted of group 1 includes; Plumeria obtuse Linn., P. pudica Jacq., P. rubra L., Odontadenia macrantha (Roem. & Schult') Markgr., Kopsia fruticosa (Kerr) A. DC.), Carissa carandas L., Beaumontia grandiflora Wall., Cerbera odollam Gaertn., Dyera costulata (Miq.) Hook. f. Group 2 includes; Catharanthus roseus (L.) Don, Tabernaemontana pandacaqui Lam., Wrightia religiosa Benth., W. antidysenterica R. Br., Holarrhena pubescens Wall. ex G. Don, Alstonia scholaris (L.) R. Br. Group 3 includes; Nerium indicum Mill., Alyxia reinwardtii Blume., Strophanthus gratus Franch., Thevetia peruviana (Pers.) Schum. Group 4 includes; Allamanda blanchetii A. DC., A. cathartica L. and group 5 includes; Adenium obesum (Forssk.) Roem.& Schult. In terms of morphology, the comparison of figures found that the relationship of Apocynaceae DNA could not be harmonized clearly moreover, RAPD can be used as teaching aids in classroom as electronic book (E-book) for teaching science for Mattayom 6 students. 2018-04-10T06:56:36Z 2018-04-10T06:56:36Z 2558-05 Independent Study (IS) http://cmuir.cmu.ac.th/jspui/handle/6653943832/46079 th เชียงใหม่ : บัณฑิตวิทยาลัย มหาวิทยาลัยเชียงใหม่ |
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ลายพิมพ์ ดีเอ็นเอ ลั่นทม |
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ลายพิมพ์ ดีเอ็นเอ ลั่นทม เจนใจ สุธีพรวิโรจน์ ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA |
description |
Plants in family Apocynaceae, such as Plumeria sp., Adenium sp., Alstonia sp., Catharanthus sp. and Allamanda sp. are very famous as a household trees. The dominant feature of these plants were white latex which were in all parts of the trees but they have different features depending on their parts such as size, flower and leave. DNA relationship among some plants in family Apocynaceae was studied by RAPD technique. The efficiency of forty primers experimental found that the proper primer for making DNA fingerprint was nine primers which were O-01, O-18, OPB-07, OPB-08, OPD-20, OPW-09, R-37, R-55 and R-105. They produce 276 polymorphic DNA bands. The analysis of DNA fingerprint data was found that the relationship of DNA by using 3.2 phylic program and Tree Veiw 1.6.6. The result could be grouped by unweighted pair group method with arithmetic average (UPGMA) in five groups consisted of group 1 includes; Plumeria obtuse Linn., P. pudica Jacq., P. rubra L., Odontadenia macrantha (Roem. & Schult') Markgr., Kopsia fruticosa (Kerr) A. DC.), Carissa carandas L., Beaumontia grandiflora Wall., Cerbera odollam Gaertn., Dyera costulata (Miq.) Hook. f. Group 2 includes; Catharanthus roseus (L.) Don, Tabernaemontana pandacaqui Lam., Wrightia religiosa Benth., W. antidysenterica R. Br., Holarrhena pubescens Wall. ex G. Don, Alstonia scholaris (L.) R. Br. Group 3 includes; Nerium indicum Mill., Alyxia reinwardtii Blume., Strophanthus gratus Franch., Thevetia peruviana (Pers.) Schum. Group 4 includes; Allamanda blanchetii A. DC., A. cathartica L. and group 5 includes; Adenium obesum (Forssk.) Roem.& Schult. In terms of morphology, the comparison of figures found that the relationship of Apocynaceae DNA could not be harmonized clearly moreover, RAPD can be used as teaching aids in classroom as electronic book (E-book) for teaching science for Mattayom 6 students. |
author2 |
สิริพร โรจน์อารยานนท์ |
author_facet |
สิริพร โรจน์อารยานนท์ เจนใจ สุธีพรวิโรจน์ |
format |
Independent Study |
author |
เจนใจ สุธีพรวิโรจน์ |
author_sort |
เจนใจ สุธีพรวิโรจน์ |
title |
ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA |
title_short |
ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA |
title_full |
ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA |
title_fullStr |
ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA |
title_full_unstemmed |
ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA |
title_sort |
ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค randomly amplified polymorphic dna |
publisher |
เชียงใหม่ : บัณฑิตวิทยาลัย มหาวิทยาลัยเชียงใหม่ |
publishDate |
2018 |
url |
http://cmuir.cmu.ac.th/jspui/handle/6653943832/46079 |
_version_ |
1681421684130709504 |