ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA

Plants in family Apocynaceae, such as Plumeria sp., Adenium sp., Alstonia sp., Catharanthus sp. and Allamanda sp. are very famous as a household trees. The dominant feature of these plants were white latex which were in all parts of the trees but they have different features depending on their part...

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Main Author: เจนใจ สุธีพรวิโรจน์
Other Authors: สิริพร โรจน์อารยานนท์
Format: Independent Study
Language:Thai
Published: เชียงใหม่ : บัณฑิตวิทยาลัย มหาวิทยาลัยเชียงใหม่ 2018
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Online Access:http://cmuir.cmu.ac.th/jspui/handle/6653943832/46079
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Institution: Chiang Mai University
Language: Thai
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spelling th-cmuir.6653943832-460792018-04-10T20:08:25Z ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA DNA Fingerprinting of Some Plants in Family Apocynaceae by Randomly Amplified Polymorphic DNA Technique เจนใจ สุธีพรวิโรจน์ สิริพร โรจน์อารยานนท์ ลายพิมพ์ ดีเอ็นเอ ลั่นทม Plants in family Apocynaceae, such as Plumeria sp., Adenium sp., Alstonia sp., Catharanthus sp. and Allamanda sp. are very famous as a household trees. The dominant feature of these plants were white latex which were in all parts of the trees but they have different features depending on their parts such as size, flower and leave. DNA relationship among some plants in family Apocynaceae was studied by RAPD technique. The efficiency of forty primers experimental found that the proper primer for making DNA fingerprint was nine primers which were O-01, O-18, OPB-07, OPB-08, OPD-20, OPW-09, R-37, R-55 and R-105. They produce 276 polymorphic DNA bands. The analysis of DNA fingerprint data was found that the relationship of DNA by using 3.2 phylic program and Tree Veiw 1.6.6. The result could be grouped by unweighted pair group method with arithmetic average (UPGMA) in five groups consisted of group 1 includes; Plumeria obtuse Linn., P. pudica Jacq., P. rubra L., Odontadenia macrantha (Roem. & Schult') Markgr., Kopsia fruticosa (Kerr) A. DC.), Carissa carandas L., Beaumontia grandiflora Wall., Cerbera odollam Gaertn., Dyera costulata (Miq.) Hook. f. Group 2 includes; Catharanthus roseus (L.) Don, Tabernaemontana pandacaqui Lam., Wrightia religiosa Benth., W. antidysenterica R. Br., Holarrhena pubescens Wall. ex G. Don, Alstonia scholaris (L.) R. Br. Group 3 includes; Nerium indicum Mill., Alyxia reinwardtii Blume., Strophanthus gratus Franch., Thevetia peruviana (Pers.) Schum. Group 4 includes; Allamanda blanchetii A. DC., A. cathartica L. and group 5 includes; Adenium obesum (Forssk.) Roem.& Schult. In terms of morphology, the comparison of figures found that the relationship of Apocynaceae DNA could not be harmonized clearly moreover, RAPD can be used as teaching aids in classroom as electronic book (E-book) for teaching science for Mattayom 6 students. 2018-04-10T06:56:36Z 2018-04-10T06:56:36Z 2558-05 Independent Study (IS) http://cmuir.cmu.ac.th/jspui/handle/6653943832/46079 th เชียงใหม่ : บัณฑิตวิทยาลัย มหาวิทยาลัยเชียงใหม่
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language Thai
topic ลายพิมพ์
ดีเอ็นเอ
ลั่นทม
spellingShingle ลายพิมพ์
ดีเอ็นเอ
ลั่นทม
เจนใจ สุธีพรวิโรจน์
ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA
description Plants in family Apocynaceae, such as Plumeria sp., Adenium sp., Alstonia sp., Catharanthus sp. and Allamanda sp. are very famous as a household trees. The dominant feature of these plants were white latex which were in all parts of the trees but they have different features depending on their parts such as size, flower and leave. DNA relationship among some plants in family Apocynaceae was studied by RAPD technique. The efficiency of forty primers experimental found that the proper primer for making DNA fingerprint was nine primers which were O-01, O-18, OPB-07, OPB-08, OPD-20, OPW-09, R-37, R-55 and R-105. They produce 276 polymorphic DNA bands. The analysis of DNA fingerprint data was found that the relationship of DNA by using 3.2 phylic program and Tree Veiw 1.6.6. The result could be grouped by unweighted pair group method with arithmetic average (UPGMA) in five groups consisted of group 1 includes; Plumeria obtuse Linn., P. pudica Jacq., P. rubra L., Odontadenia macrantha (Roem. & Schult') Markgr., Kopsia fruticosa (Kerr) A. DC.), Carissa carandas L., Beaumontia grandiflora Wall., Cerbera odollam Gaertn., Dyera costulata (Miq.) Hook. f. Group 2 includes; Catharanthus roseus (L.) Don, Tabernaemontana pandacaqui Lam., Wrightia religiosa Benth., W. antidysenterica R. Br., Holarrhena pubescens Wall. ex G. Don, Alstonia scholaris (L.) R. Br. Group 3 includes; Nerium indicum Mill., Alyxia reinwardtii Blume., Strophanthus gratus Franch., Thevetia peruviana (Pers.) Schum. Group 4 includes; Allamanda blanchetii A. DC., A. cathartica L. and group 5 includes; Adenium obesum (Forssk.) Roem.& Schult. In terms of morphology, the comparison of figures found that the relationship of Apocynaceae DNA could not be harmonized clearly moreover, RAPD can be used as teaching aids in classroom as electronic book (E-book) for teaching science for Mattayom 6 students.
author2 สิริพร โรจน์อารยานนท์
author_facet สิริพร โรจน์อารยานนท์
เจนใจ สุธีพรวิโรจน์
format Independent Study
author เจนใจ สุธีพรวิโรจน์
author_sort เจนใจ สุธีพรวิโรจน์
title ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA
title_short ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA
title_full ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA
title_fullStr ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA
title_full_unstemmed ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค Randomly Amplified Polymorphic DNA
title_sort ลายพิมพ์ดีเอ็นเอของพืชวงศ์ลั่นทมบางชนิดด้วยเทคนิค randomly amplified polymorphic dna
publisher เชียงใหม่ : บัณฑิตวิทยาลัย มหาวิทยาลัยเชียงใหม่
publishDate 2018
url http://cmuir.cmu.ac.th/jspui/handle/6653943832/46079
_version_ 1681421684130709504