Midgut bacterial communities in the giant Asian honeybee (Apis dorsata) across 4 developmental stages: A comparative study

© 2015 Institute of Zoology, Chinese Academy of Sciences Bacterial communities are known to play important roles during the developmental stages of insects, but current knowledge of bacteria associated with the midgut of Apis dorsata, the giant Asian honeybee, is limited. Using polymerase chain reac...

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Main Authors: Prakaimuk Saraithong, Yihong Li, Kanokporn Saenphet, Zhou Chen, Panuwan Chantawannakul
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/46254
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-462542018-04-25T07:28:10Z Midgut bacterial communities in the giant Asian honeybee (Apis dorsata) across 4 developmental stages: A comparative study Prakaimuk Saraithong Yihong Li Kanokporn Saenphet Zhou Chen Panuwan Chantawannakul Biochemistry, Genetics and Molecular Biology Agricultural and Biological Sciences © 2015 Institute of Zoology, Chinese Academy of Sciences Bacterial communities are known to play important roles during the developmental stages of insects, but current knowledge of bacteria associated with the midgut of Apis dorsata, the giant Asian honeybee, is limited. Using polymerase chain reaction-denaturing gradient gel electrophoresis analysis (PCR-DGGE) and 16S rRNA sequencing, the aim of this study was to determine the dynamics of bacterial community structure across four A. dorsata life stages in different geographical locations. The results reveal that bacterial diversity increased as the bee progressed through larval stage to newly emerged worker and old worker. However, in the pupal stage, no bands identified as bacteria could be observed. Overall, 2 bacterial phyla (Proteobacteria and Firmicutes) and 4 classes (Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, and Bacilli) were identified, but the frequency varied among the different stages and locations. The classes of Gammaproteobacteria and Bacilli dominated among larval, newly emerged worker and old worker developmental stages. 2018-04-25T06:51:58Z 2018-04-25T06:51:58Z 2017-02-01 Journal 17447917 16729609 2-s2.0-84955567087 10.1111/1744-7917.12271 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84955567087&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46254
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Agricultural and Biological Sciences
spellingShingle Biochemistry, Genetics and Molecular Biology
Agricultural and Biological Sciences
Prakaimuk Saraithong
Yihong Li
Kanokporn Saenphet
Zhou Chen
Panuwan Chantawannakul
Midgut bacterial communities in the giant Asian honeybee (Apis dorsata) across 4 developmental stages: A comparative study
description © 2015 Institute of Zoology, Chinese Academy of Sciences Bacterial communities are known to play important roles during the developmental stages of insects, but current knowledge of bacteria associated with the midgut of Apis dorsata, the giant Asian honeybee, is limited. Using polymerase chain reaction-denaturing gradient gel electrophoresis analysis (PCR-DGGE) and 16S rRNA sequencing, the aim of this study was to determine the dynamics of bacterial community structure across four A. dorsata life stages in different geographical locations. The results reveal that bacterial diversity increased as the bee progressed through larval stage to newly emerged worker and old worker. However, in the pupal stage, no bands identified as bacteria could be observed. Overall, 2 bacterial phyla (Proteobacteria and Firmicutes) and 4 classes (Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, and Bacilli) were identified, but the frequency varied among the different stages and locations. The classes of Gammaproteobacteria and Bacilli dominated among larval, newly emerged worker and old worker developmental stages.
format Journal
author Prakaimuk Saraithong
Yihong Li
Kanokporn Saenphet
Zhou Chen
Panuwan Chantawannakul
author_facet Prakaimuk Saraithong
Yihong Li
Kanokporn Saenphet
Zhou Chen
Panuwan Chantawannakul
author_sort Prakaimuk Saraithong
title Midgut bacterial communities in the giant Asian honeybee (Apis dorsata) across 4 developmental stages: A comparative study
title_short Midgut bacterial communities in the giant Asian honeybee (Apis dorsata) across 4 developmental stages: A comparative study
title_full Midgut bacterial communities in the giant Asian honeybee (Apis dorsata) across 4 developmental stages: A comparative study
title_fullStr Midgut bacterial communities in the giant Asian honeybee (Apis dorsata) across 4 developmental stages: A comparative study
title_full_unstemmed Midgut bacterial communities in the giant Asian honeybee (Apis dorsata) across 4 developmental stages: A comparative study
title_sort midgut bacterial communities in the giant asian honeybee (apis dorsata) across 4 developmental stages: a comparative study
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84955567087&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/46254
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