Quercetin promotes the expression of genes involved in phagocytosisin bovine neutrophils

© 2017 Suphakit Srikok, Sukij Nambut, Kanruethai Wongsawan and Phongsakorn Chuammitri. This study was conducted to explore the effect of Quercetin (QH) on phagocytosis of bovine neutrophils. Neutrophils were isolated from fifteen multiparous Holstein cows. We in vitro treated neutrophils with PBS or...

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Main Authors: Suphakit Srikok, Sukij Nambut, Kanruethai Wongsawan, Phongsakorn Chuammitri
Format: Journal
Published: 2018
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spelling th-cmuir.6653943832-462652018-04-25T07:30:00Z Quercetin promotes the expression of genes involved in phagocytosisin bovine neutrophils Suphakit Srikok Sukij Nambut Kanruethai Wongsawan Phongsakorn Chuammitri Agricultural and Biological Sciences © 2017 Suphakit Srikok, Sukij Nambut, Kanruethai Wongsawan and Phongsakorn Chuammitri. This study was conducted to explore the effect of Quercetin (QH) on phagocytosis of bovine neutrophils. Neutrophils were isolated from fifteen multiparous Holstein cows. We in vitro treated neutrophils with PBS or 50 μM of Quercetin (QH) or Cytochalasin B (CytB), as phagocytosis inhibitor, prior to monitoring phagocytosis of Escherichia coli by flow cytometry and microscopic examination. Additionally, the expressions of CORO1A, CYBA (gp91 phox ), LAMP1, RAB7A, RAC1 and PAK1 mRNA were analyzed by real-time PCR. In the time-course experiment, treated neutrophils were allowed to co-cultured with live bacteria for 30, 60 and 90 min before measuring gene expressions. The expression levels of IL-1 β and TNF genes in order to demonstrate the antiinflammatory property of quercetin were assessed by conventional RTPCR. The results of flow cytometry and microscopic examination showed that the percentage of neutrophils performing phagocytosis were significantly higher in QH group in comparison with other groups. We reported here that mRNA expressions of CORO1A, CYBA, LAMP1, RAB7A, RAC1 and PAK1 genes involved in phagocytosis, were significantly up-regulated in QH group. As expected, CytB group had profound down-regulation of genes with one exception in PAK1. From our observations, the expression levels of phagocytic process genes in the QH group were optimum at 60 min and started to decline at 90 min of incubation. The data also indicated that quercetin inhibited inflammation by reducing the expressions of both IL-1_ and TNF. In conclusion, the information in our experiments conclude that quercetin has the ability to boost the expression of genes involved in phagocytosis while reduces the expression of proinflammatory genes. 2018-04-25T06:52:03Z 2018-04-25T06:52:03Z 2017-01-01 Journal 15574563 15574555 2-s2.0-85026797409 10.3844/ajavsp.2017.85.95 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85026797409&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46265
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Suphakit Srikok
Sukij Nambut
Kanruethai Wongsawan
Phongsakorn Chuammitri
Quercetin promotes the expression of genes involved in phagocytosisin bovine neutrophils
description © 2017 Suphakit Srikok, Sukij Nambut, Kanruethai Wongsawan and Phongsakorn Chuammitri. This study was conducted to explore the effect of Quercetin (QH) on phagocytosis of bovine neutrophils. Neutrophils were isolated from fifteen multiparous Holstein cows. We in vitro treated neutrophils with PBS or 50 μM of Quercetin (QH) or Cytochalasin B (CytB), as phagocytosis inhibitor, prior to monitoring phagocytosis of Escherichia coli by flow cytometry and microscopic examination. Additionally, the expressions of CORO1A, CYBA (gp91 phox ), LAMP1, RAB7A, RAC1 and PAK1 mRNA were analyzed by real-time PCR. In the time-course experiment, treated neutrophils were allowed to co-cultured with live bacteria for 30, 60 and 90 min before measuring gene expressions. The expression levels of IL-1 β and TNF genes in order to demonstrate the antiinflammatory property of quercetin were assessed by conventional RTPCR. The results of flow cytometry and microscopic examination showed that the percentage of neutrophils performing phagocytosis were significantly higher in QH group in comparison with other groups. We reported here that mRNA expressions of CORO1A, CYBA, LAMP1, RAB7A, RAC1 and PAK1 genes involved in phagocytosis, were significantly up-regulated in QH group. As expected, CytB group had profound down-regulation of genes with one exception in PAK1. From our observations, the expression levels of phagocytic process genes in the QH group were optimum at 60 min and started to decline at 90 min of incubation. The data also indicated that quercetin inhibited inflammation by reducing the expressions of both IL-1_ and TNF. In conclusion, the information in our experiments conclude that quercetin has the ability to boost the expression of genes involved in phagocytosis while reduces the expression of proinflammatory genes.
format Journal
author Suphakit Srikok
Sukij Nambut
Kanruethai Wongsawan
Phongsakorn Chuammitri
author_facet Suphakit Srikok
Sukij Nambut
Kanruethai Wongsawan
Phongsakorn Chuammitri
author_sort Suphakit Srikok
title Quercetin promotes the expression of genes involved in phagocytosisin bovine neutrophils
title_short Quercetin promotes the expression of genes involved in phagocytosisin bovine neutrophils
title_full Quercetin promotes the expression of genes involved in phagocytosisin bovine neutrophils
title_fullStr Quercetin promotes the expression of genes involved in phagocytosisin bovine neutrophils
title_full_unstemmed Quercetin promotes the expression of genes involved in phagocytosisin bovine neutrophils
title_sort quercetin promotes the expression of genes involved in phagocytosisin bovine neutrophils
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85026797409&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/46265
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