Analysis of complete genome sequences of G9P[19] rotavirus strains from human and piglet with diarrhea provides evidence for whole-genome interspecies transmission of nonreassorted porcine rotavirus
© 2016 Whole genomes of G9P[19] human (RVA/Human-wt/THA/CMH-S070-13/2013/G9P[19] ) and porcine (RVA/Pig-wt/THA/CMP-015-12/2012/G9P[19]) rotaviruses concurrently detected in the same geographical area in northern Thailand were sequenced and analyzed for their genetic relationships using bioinformatic...
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th-cmuir.6653943832-462992018-04-25T07:33:33Z Analysis of complete genome sequences of G9P[19] rotavirus strains from human and piglet with diarrhea provides evidence for whole-genome interspecies transmission of nonreassorted porcine rotavirus Arpaporn Yodmeeklin Pattara Khamrin Watchaporn Chuchaona Kattareeya Kumthip Aphisek Kongkaew Ratchaya Vachirachewin Shoko Okitsu Hiroshi Ushijima Niwat Maneekarn Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Agricultural and Biological Sciences © 2016 Whole genomes of G9P[19] human (RVA/Human-wt/THA/CMH-S070-13/2013/G9P[19] ) and porcine (RVA/Pig-wt/THA/CMP-015-12/2012/G9P[19]) rotaviruses concurrently detected in the same geographical area in northern Thailand were sequenced and analyzed for their genetic relationships using bioinformatic tools. The complete genome sequence of human rotavirus RVA/Human-wt/THA/CMH-S070-13/2013/G9P[19] was most closely related to those of porcine rotavirus RVA/Pig-wt/THA/CMP-015-12/2012/G9P[19] and to those of porcine-like human and porcine rotaviruses reference strains than to those of human rotavirus reference strains. The genotype constellation of G9P[19] detected in human and piglet were identical and displayed as the G9-P[19]-I5-R1-C1-M1-A8-N1-T1-E1-H1 genotypes with the nucleotide sequence identities of VP7, VP4, VP6, VP1, VP2, VP3, NSP1, NSP2, NSP3, NSP4, and NSP5 at 99.0%, 99.5%, 93.2%, 97.7%, 97.7%, 85.6%, 89.5%, 93.2%, 92.9%, 94.0%, and 98.1%, respectively. The findings indicate that human rotavirus strain RVA/Human-wt/THA/CMH-S070-13/2013/G9P[19] containing the genome segments of porcine genetic backbone is most likely a human rotavirus of porcine origin. Our data provide an evidence of interspecies transmission and whole-genome transmission of nonreassorted G9P[19] porcine RVA to h uman occurring in nature in northern Thailand. 2018-04-25T06:52:28Z 2018-04-25T06:52:28Z 2017-01-01 Journal 15677257 15671348 2-s2.0-84998631545 10.1016/j.meegid.2016.11.021 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84998631545&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46299 |
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Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Agricultural and Biological Sciences Arpaporn Yodmeeklin Pattara Khamrin Watchaporn Chuchaona Kattareeya Kumthip Aphisek Kongkaew Ratchaya Vachirachewin Shoko Okitsu Hiroshi Ushijima Niwat Maneekarn Analysis of complete genome sequences of G9P[19] rotavirus strains from human and piglet with diarrhea provides evidence for whole-genome interspecies transmission of nonreassorted porcine rotavirus |
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© 2016 Whole genomes of G9P[19] human (RVA/Human-wt/THA/CMH-S070-13/2013/G9P[19] ) and porcine (RVA/Pig-wt/THA/CMP-015-12/2012/G9P[19]) rotaviruses concurrently detected in the same geographical area in northern Thailand were sequenced and analyzed for their genetic relationships using bioinformatic tools. The complete genome sequence of human rotavirus RVA/Human-wt/THA/CMH-S070-13/2013/G9P[19] was most closely related to those of porcine rotavirus RVA/Pig-wt/THA/CMP-015-12/2012/G9P[19] and to those of porcine-like human and porcine rotaviruses reference strains than to those of human rotavirus reference strains. The genotype constellation of G9P[19] detected in human and piglet were identical and displayed as the G9-P[19]-I5-R1-C1-M1-A8-N1-T1-E1-H1 genotypes with the nucleotide sequence identities of VP7, VP4, VP6, VP1, VP2, VP3, NSP1, NSP2, NSP3, NSP4, and NSP5 at 99.0%, 99.5%, 93.2%, 97.7%, 97.7%, 85.6%, 89.5%, 93.2%, 92.9%, 94.0%, and 98.1%, respectively. The findings indicate that human rotavirus strain RVA/Human-wt/THA/CMH-S070-13/2013/G9P[19] containing the genome segments of porcine genetic backbone is most likely a human rotavirus of porcine origin. Our data provide an evidence of interspecies transmission and whole-genome transmission of nonreassorted G9P[19] porcine RVA to h uman occurring in nature in northern Thailand. |
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Journal |
author |
Arpaporn Yodmeeklin Pattara Khamrin Watchaporn Chuchaona Kattareeya Kumthip Aphisek Kongkaew Ratchaya Vachirachewin Shoko Okitsu Hiroshi Ushijima Niwat Maneekarn |
author_facet |
Arpaporn Yodmeeklin Pattara Khamrin Watchaporn Chuchaona Kattareeya Kumthip Aphisek Kongkaew Ratchaya Vachirachewin Shoko Okitsu Hiroshi Ushijima Niwat Maneekarn |
author_sort |
Arpaporn Yodmeeklin |
title |
Analysis of complete genome sequences of G9P[19] rotavirus strains from human and piglet with diarrhea provides evidence for whole-genome interspecies transmission of nonreassorted porcine rotavirus |
title_short |
Analysis of complete genome sequences of G9P[19] rotavirus strains from human and piglet with diarrhea provides evidence for whole-genome interspecies transmission of nonreassorted porcine rotavirus |
title_full |
Analysis of complete genome sequences of G9P[19] rotavirus strains from human and piglet with diarrhea provides evidence for whole-genome interspecies transmission of nonreassorted porcine rotavirus |
title_fullStr |
Analysis of complete genome sequences of G9P[19] rotavirus strains from human and piglet with diarrhea provides evidence for whole-genome interspecies transmission of nonreassorted porcine rotavirus |
title_full_unstemmed |
Analysis of complete genome sequences of G9P[19] rotavirus strains from human and piglet with diarrhea provides evidence for whole-genome interspecies transmission of nonreassorted porcine rotavirus |
title_sort |
analysis of complete genome sequences of g9p[19] rotavirus strains from human and piglet with diarrhea provides evidence for whole-genome interspecies transmission of nonreassorted porcine rotavirus |
publishDate |
2018 |
url |
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84998631545&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46299 |
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1681422848512491520 |