Detection of bacterial communities in volatile-organic-compound (VOC)-contaminated soil in an industrial estate in eastern Thailand by PCR-DGGE analysis

© 2017, Chiang Mai University. All rights reserved. The prolonged contamination and accumulation of volatile organic compounds (VOCs) in soil and groundwater around industrial estate areas can have a negative impact on human health and the environment. In situ bioremediation by microbial activities...

Full description

Saved in:
Bibliographic Details
Main Authors: Manatsawat Sriwichai, Fairda Malem, Mujalin K. Pholchan, Sakunnee Bovonsombut
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85023758360&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/46377
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-46377
record_format dspace
spelling th-cmuir.6653943832-463772018-04-25T07:22:57Z Detection of bacterial communities in volatile-organic-compound (VOC)-contaminated soil in an industrial estate in eastern Thailand by PCR-DGGE analysis Manatsawat Sriwichai Fairda Malem Mujalin K. Pholchan Sakunnee Bovonsombut Chemistry Materials Science Mathematics Agricultural and Biological Sciences © 2017, Chiang Mai University. All rights reserved. The prolonged contamination and accumulation of volatile organic compounds (VOCs) in soil and groundwater around industrial estate areas can have a negative impact on human health and the environment. In situ bioremediation by microbial activities is one of the most effective ways to remove soil pollutants. In order to promote the bioremediation process by nutrient application, it is important to understand the microbial community in the affected area. In this study, the diversity of bacterial communities in VOC-contaminated soil collected from eight sites within an industrial estate in the Eastern part of Thailand was determined by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis. The bacterial diversities in soil samples were evaluated in three enrichment media: tryptic soy broth, nutrient broth, and half-strength nutrient broth. Primers targeting 16S rRNA gene fragments were used. A total bacteria count was also performed in order to determine whether the number of bacteria were sufficient for bioremediation. We found that enrichment of soil with tryptic soy broth resulted in the greatest bacterial diversity. The bacterial communities in VOC-contaminated soil consisted mainly of Gram-negative Proteobacteria including Gamma- and Beta-proteobacteria, with genus Pseudomonas being prominent, and a Gram-positive member of phylum Firmicutes which was identified as Bacillus. The bacterial counts in most samples were higher than 3 logCFU⋅g -1 , which were high enough for in situ bioremediation. The results from this study could be used when considering nutrient application to increase the efficiency of bioremediation. 2018-04-25T06:54:04Z 2018-04-25T06:54:04Z 2017-07-01 Journal 01252526 2-s2.0-85023758360 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85023758360&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46377
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Chemistry
Materials Science
Mathematics
Agricultural and Biological Sciences
spellingShingle Chemistry
Materials Science
Mathematics
Agricultural and Biological Sciences
Manatsawat Sriwichai
Fairda Malem
Mujalin K. Pholchan
Sakunnee Bovonsombut
Detection of bacterial communities in volatile-organic-compound (VOC)-contaminated soil in an industrial estate in eastern Thailand by PCR-DGGE analysis
description © 2017, Chiang Mai University. All rights reserved. The prolonged contamination and accumulation of volatile organic compounds (VOCs) in soil and groundwater around industrial estate areas can have a negative impact on human health and the environment. In situ bioremediation by microbial activities is one of the most effective ways to remove soil pollutants. In order to promote the bioremediation process by nutrient application, it is important to understand the microbial community in the affected area. In this study, the diversity of bacterial communities in VOC-contaminated soil collected from eight sites within an industrial estate in the Eastern part of Thailand was determined by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis. The bacterial diversities in soil samples were evaluated in three enrichment media: tryptic soy broth, nutrient broth, and half-strength nutrient broth. Primers targeting 16S rRNA gene fragments were used. A total bacteria count was also performed in order to determine whether the number of bacteria were sufficient for bioremediation. We found that enrichment of soil with tryptic soy broth resulted in the greatest bacterial diversity. The bacterial communities in VOC-contaminated soil consisted mainly of Gram-negative Proteobacteria including Gamma- and Beta-proteobacteria, with genus Pseudomonas being prominent, and a Gram-positive member of phylum Firmicutes which was identified as Bacillus. The bacterial counts in most samples were higher than 3 logCFU⋅g -1 , which were high enough for in situ bioremediation. The results from this study could be used when considering nutrient application to increase the efficiency of bioremediation.
format Journal
author Manatsawat Sriwichai
Fairda Malem
Mujalin K. Pholchan
Sakunnee Bovonsombut
author_facet Manatsawat Sriwichai
Fairda Malem
Mujalin K. Pholchan
Sakunnee Bovonsombut
author_sort Manatsawat Sriwichai
title Detection of bacterial communities in volatile-organic-compound (VOC)-contaminated soil in an industrial estate in eastern Thailand by PCR-DGGE analysis
title_short Detection of bacterial communities in volatile-organic-compound (VOC)-contaminated soil in an industrial estate in eastern Thailand by PCR-DGGE analysis
title_full Detection of bacterial communities in volatile-organic-compound (VOC)-contaminated soil in an industrial estate in eastern Thailand by PCR-DGGE analysis
title_fullStr Detection of bacterial communities in volatile-organic-compound (VOC)-contaminated soil in an industrial estate in eastern Thailand by PCR-DGGE analysis
title_full_unstemmed Detection of bacterial communities in volatile-organic-compound (VOC)-contaminated soil in an industrial estate in eastern Thailand by PCR-DGGE analysis
title_sort detection of bacterial communities in volatile-organic-compound (voc)-contaminated soil in an industrial estate in eastern thailand by pcr-dgge analysis
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85023758360&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/46377
_version_ 1681422863136980992