Extracellular protease derived from lactic acid bacteria stimulates the fermentative lactic acid production from the by-products of rice as a biomass refinery function

© 2016 The Society for Biotechnology, Japan A lactic acid producing bacterium, Lactobacillus rhamnosus M-23, newly isolated from a rice washing drainage storage tank was found to produce L-(+)-lactic acid from a non-sterilized mixture of rice washing drainage and rice bran without any additions of n...

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Main Authors: Masanori Watanabe, Charin Techapun, Ampin Kuntiya, Noppol Leksawasdi, Phisit Seesuriyachan, Thanongsak Chaiyaso, Shinji Takenaka, Isamu Maeda, Masahiro Koyama, Kozo Nakamura
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/46438
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-464382018-04-25T07:28:22Z Extracellular protease derived from lactic acid bacteria stimulates the fermentative lactic acid production from the by-products of rice as a biomass refinery function Masanori Watanabe Charin Techapun Ampin Kuntiya Noppol Leksawasdi Phisit Seesuriyachan Thanongsak Chaiyaso Shinji Takenaka Isamu Maeda Masahiro Koyama Kozo Nakamura Chemical Engineering Immunology and Microbiology Agricultural and Biological Sciences © 2016 The Society for Biotechnology, Japan A lactic acid producing bacterium, Lactobacillus rhamnosus M-23, newly isolated from a rice washing drainage storage tank was found to produce L-(+)-lactic acid from a non-sterilized mixture of rice washing drainage and rice bran without any additions of nutrients under the simultaneous saccharification and fermentation (SSF) process. This strain has the ability to utilize the non-sterilized rice washing drainage and rice bran as a source of carbohydrate, saccharifying enzymes and nutrients for lactic acid production. Observation of extracellular protease activity in SSF culture broth showed that a higher protease activity was present in strain M-23 than in other isolated lactic acid producing bacteria (LABs). To investigate the structural changes of solid particles of rice washing drainage throughout LAB cultivation, scanning electron microscopic (SEM) observation and Fourier transform infrared-spectroscopy (FT-IR) analysis were performed. The results of the SEM observation showed that the surface material could be removed from solid particles of rice washing drainage treated by culture broth (supernatant) of strain M-23, thus exposing the crystal structure of the starch particle surface. The results of the FT-IR analysis revealed that the specific transmittance decrease of the C[sbnd]C and C[sbnd] O stretching and OH[sbnd] group of the solid particles of the rice washing drainage were highly correlated with the produc ed lactic acid concentration and extracellular protease activity, respectively. These results demonstrate the high lactic acid producing ability of strain M-23 from a non-sterilized mixture of rice washing drainage and rice bran under the SSF condition due to the removal of proteinaceous material and exposure of the starch particle surface by extracellular protease. 2018-04-25T06:54:56Z 2018-04-25T06:54:56Z 2017-02-01 Journal 13474421 13891723 2-s2.0-84994527572 10.1016/j.jbiosc.2016.08.011 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84994527572&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46438
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Chemical Engineering
Immunology and Microbiology
Agricultural and Biological Sciences
spellingShingle Chemical Engineering
Immunology and Microbiology
Agricultural and Biological Sciences
Masanori Watanabe
Charin Techapun
Ampin Kuntiya
Noppol Leksawasdi
Phisit Seesuriyachan
Thanongsak Chaiyaso
Shinji Takenaka
Isamu Maeda
Masahiro Koyama
Kozo Nakamura
Extracellular protease derived from lactic acid bacteria stimulates the fermentative lactic acid production from the by-products of rice as a biomass refinery function
description © 2016 The Society for Biotechnology, Japan A lactic acid producing bacterium, Lactobacillus rhamnosus M-23, newly isolated from a rice washing drainage storage tank was found to produce L-(+)-lactic acid from a non-sterilized mixture of rice washing drainage and rice bran without any additions of nutrients under the simultaneous saccharification and fermentation (SSF) process. This strain has the ability to utilize the non-sterilized rice washing drainage and rice bran as a source of carbohydrate, saccharifying enzymes and nutrients for lactic acid production. Observation of extracellular protease activity in SSF culture broth showed that a higher protease activity was present in strain M-23 than in other isolated lactic acid producing bacteria (LABs). To investigate the structural changes of solid particles of rice washing drainage throughout LAB cultivation, scanning electron microscopic (SEM) observation and Fourier transform infrared-spectroscopy (FT-IR) analysis were performed. The results of the SEM observation showed that the surface material could be removed from solid particles of rice washing drainage treated by culture broth (supernatant) of strain M-23, thus exposing the crystal structure of the starch particle surface. The results of the FT-IR analysis revealed that the specific transmittance decrease of the C[sbnd]C and C[sbnd] O stretching and OH[sbnd] group of the solid particles of the rice washing drainage were highly correlated with the produc ed lactic acid concentration and extracellular protease activity, respectively. These results demonstrate the high lactic acid producing ability of strain M-23 from a non-sterilized mixture of rice washing drainage and rice bran under the SSF condition due to the removal of proteinaceous material and exposure of the starch particle surface by extracellular protease.
format Journal
author Masanori Watanabe
Charin Techapun
Ampin Kuntiya
Noppol Leksawasdi
Phisit Seesuriyachan
Thanongsak Chaiyaso
Shinji Takenaka
Isamu Maeda
Masahiro Koyama
Kozo Nakamura
author_facet Masanori Watanabe
Charin Techapun
Ampin Kuntiya
Noppol Leksawasdi
Phisit Seesuriyachan
Thanongsak Chaiyaso
Shinji Takenaka
Isamu Maeda
Masahiro Koyama
Kozo Nakamura
author_sort Masanori Watanabe
title Extracellular protease derived from lactic acid bacteria stimulates the fermentative lactic acid production from the by-products of rice as a biomass refinery function
title_short Extracellular protease derived from lactic acid bacteria stimulates the fermentative lactic acid production from the by-products of rice as a biomass refinery function
title_full Extracellular protease derived from lactic acid bacteria stimulates the fermentative lactic acid production from the by-products of rice as a biomass refinery function
title_fullStr Extracellular protease derived from lactic acid bacteria stimulates the fermentative lactic acid production from the by-products of rice as a biomass refinery function
title_full_unstemmed Extracellular protease derived from lactic acid bacteria stimulates the fermentative lactic acid production from the by-products of rice as a biomass refinery function
title_sort extracellular protease derived from lactic acid bacteria stimulates the fermentative lactic acid production from the by-products of rice as a biomass refinery function
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84994527572&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/46438
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