Properties of macerated herbal oil

© 2017 The Author(s). Introduction: The addition of herbs into hot sesame oil could increase the oil-pulling efficiency of sesame oil. The aim of present study was to modify the proportion of herbs and sesame oil with the addition of other ingredients including menthol, camphor, and borneol and impr...

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Main Authors: Fahsai Kantawong, Supawatchara Singhatong, Aomjai Srilamay, Kantarose Boonyuen, Niroot Mooti, Phenphichar Wanachantararak, Thasaneeya Kuboki
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/46458
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-464582018-04-25T07:31:34Z Properties of macerated herbal oil Fahsai Kantawong Supawatchara Singhatong Aomjai Srilamay Kantarose Boonyuen Niroot Mooti Phenphichar Wanachantararak Thasaneeya Kuboki Agricultural and Biological Sciences © 2017 The Author(s). Introduction: The addition of herbs into hot sesame oil could increase the oil-pulling efficiency of sesame oil. The aim of present study was to modify the proportion of herbs and sesame oil with the addition of other ingredients including menthol, camphor, and borneol and improve the medicinal properties and the scent of the oil. Methods: Macerated herbal oil was prepared by heat extraction of five species of herbs (Zingiber cassumunar, Zingiber zerumbet, Plantago major Linn, Citrus hystrix, and Amomum biflorum) with hot sesame oil. The study was performed to evaluate the anti-oxidant, anti-inflammatory, and anti-bacterial properties of this macerated herbal oil. Results: Macerated herbal oil was evaluated for antioxidant activity using DPPH and ABTS assays. It was shown that at dilution 1:2 in DMSO, the macerated herbal oil had DPPH and ABTS radical scavenging activities equal to 63% and 22%, respectively. Macerated herbal oil dilution 1:8 in DMSO demonstrated ferric reducing capacity equivalent to ascorbic acid (0.208 μM) and had reducing power equivalent to butylated hydroxytoluene (BHT) 7.41 μg/mL. MTT assay was performed using immortalized human mesenchymal stem cells (HMSCs) as a cell culture model. The result indicated that the cytotoxic concentration of the macerated herbal oil was ≥ 2.5 μL/ mL in complete DMEM. Anti-inflammatory effects were evaluated using the nitrite assay and RT-PCR. It was found that the macerated herbal oil could inhibit nitrite accumulation in culture media. Change in the expression of CO X-2, Nrf2, and NF-kB in RT-PCR confirmed the antiinflammatory activity of the macerated herbal oil. Conclusion: It could be concluded that the macerated herbal oil could inhibit nitrite accumulation in culture media, which might be the inhibitory effect of the macerated herbal oil on COX-2 or Nrf2, the downstream modulator of the COX-2 pathway. Further intensive studies are needed for the optimization before bringing this macerated herbal oil into clinical application. 2018-04-25T06:55:10Z 2018-04-25T06:55:10Z 2017-01-01 Journal 22285660 22285652 2-s2.0-85019158241 10.15171/bi.2017.03 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85019158241&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46458
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Fahsai Kantawong
Supawatchara Singhatong
Aomjai Srilamay
Kantarose Boonyuen
Niroot Mooti
Phenphichar Wanachantararak
Thasaneeya Kuboki
Properties of macerated herbal oil
description © 2017 The Author(s). Introduction: The addition of herbs into hot sesame oil could increase the oil-pulling efficiency of sesame oil. The aim of present study was to modify the proportion of herbs and sesame oil with the addition of other ingredients including menthol, camphor, and borneol and improve the medicinal properties and the scent of the oil. Methods: Macerated herbal oil was prepared by heat extraction of five species of herbs (Zingiber cassumunar, Zingiber zerumbet, Plantago major Linn, Citrus hystrix, and Amomum biflorum) with hot sesame oil. The study was performed to evaluate the anti-oxidant, anti-inflammatory, and anti-bacterial properties of this macerated herbal oil. Results: Macerated herbal oil was evaluated for antioxidant activity using DPPH and ABTS assays. It was shown that at dilution 1:2 in DMSO, the macerated herbal oil had DPPH and ABTS radical scavenging activities equal to 63% and 22%, respectively. Macerated herbal oil dilution 1:8 in DMSO demonstrated ferric reducing capacity equivalent to ascorbic acid (0.208 μM) and had reducing power equivalent to butylated hydroxytoluene (BHT) 7.41 μg/mL. MTT assay was performed using immortalized human mesenchymal stem cells (HMSCs) as a cell culture model. The result indicated that the cytotoxic concentration of the macerated herbal oil was ≥ 2.5 μL/ mL in complete DMEM. Anti-inflammatory effects were evaluated using the nitrite assay and RT-PCR. It was found that the macerated herbal oil could inhibit nitrite accumulation in culture media. Change in the expression of CO X-2, Nrf2, and NF-kB in RT-PCR confirmed the antiinflammatory activity of the macerated herbal oil. Conclusion: It could be concluded that the macerated herbal oil could inhibit nitrite accumulation in culture media, which might be the inhibitory effect of the macerated herbal oil on COX-2 or Nrf2, the downstream modulator of the COX-2 pathway. Further intensive studies are needed for the optimization before bringing this macerated herbal oil into clinical application.
format Journal
author Fahsai Kantawong
Supawatchara Singhatong
Aomjai Srilamay
Kantarose Boonyuen
Niroot Mooti
Phenphichar Wanachantararak
Thasaneeya Kuboki
author_facet Fahsai Kantawong
Supawatchara Singhatong
Aomjai Srilamay
Kantarose Boonyuen
Niroot Mooti
Phenphichar Wanachantararak
Thasaneeya Kuboki
author_sort Fahsai Kantawong
title Properties of macerated herbal oil
title_short Properties of macerated herbal oil
title_full Properties of macerated herbal oil
title_fullStr Properties of macerated herbal oil
title_full_unstemmed Properties of macerated herbal oil
title_sort properties of macerated herbal oil
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85019158241&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/46458
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