Sequential injection system with amperometric immunosensor for sensitive determination of human immunoglobulin G

© 2017 Elsevier B.V. Sequential injection (SI) system incorporated with amperometric immunosensor was developed for sensitive determination of human immunoglobulin G (HIgG). A cost effective label-free immunosensor was fabricated by immobilizing anti-HIgG on a graphene oxide (GO) modified screen-pri...

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Main Authors: Chidkamon Thunkhamrak, Preeyaporn Reanpang, Kontad Ounnunkad, Jaroon Jakmunee
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/46575
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-465752018-04-25T07:21:26Z Sequential injection system with amperometric immunosensor for sensitive determination of human immunoglobulin G Chidkamon Thunkhamrak Preeyaporn Reanpang Kontad Ounnunkad Jaroon Jakmunee Agricultural and Biological Sciences © 2017 Elsevier B.V. Sequential injection (SI) system incorporated with amperometric immunosensor was developed for sensitive determination of human immunoglobulin G (HIgG). A cost effective label-free immunosensor was fabricated by immobilizing anti-HIgG on a graphene oxide (GO) modified screen-printed carbon electrode (SPCE). The developed electrode was characterized by cyclic voltammetry(CV), scanning electron microscope(SEM), and energy dispersive spectroscopy(EDS) which confirmed the selective immunointeraction of HIgG to the anti-HIgG on the electrode, thus reduced the amperometric current of [Fe(CN) 6 ] 3-/4- redox probe. The sensing electrode was placed in a designed electrochemical flow cell of SI system, where the redox probe was propelled through and the currents before and after the immunointeraction occurred were measured amperometrically by using a simple home-made amperometer. Under the optimum condition: flow rate of 2 mL min −1 , applied potential of +350 mV, [Fe(CN) 6 ] 3-/4- concentration of 10 mM and 10 min of incubation time, a linear calibration in the range of 2–100 ng mL −1 was achieved, with detection limit of 1.70 ng mL −1 . The proposed system provided good repeatability and reproducibility and the application for urine sample analysis was demonstrated. 2018-04-25T06:57:10Z 2018-04-25T06:57:10Z 2017-08-15 Journal 00399140 2-s2.0-85018266578 10.1016/j.talanta.2017.04.058 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85018266578&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46575
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Chidkamon Thunkhamrak
Preeyaporn Reanpang
Kontad Ounnunkad
Jaroon Jakmunee
Sequential injection system with amperometric immunosensor for sensitive determination of human immunoglobulin G
description © 2017 Elsevier B.V. Sequential injection (SI) system incorporated with amperometric immunosensor was developed for sensitive determination of human immunoglobulin G (HIgG). A cost effective label-free immunosensor was fabricated by immobilizing anti-HIgG on a graphene oxide (GO) modified screen-printed carbon electrode (SPCE). The developed electrode was characterized by cyclic voltammetry(CV), scanning electron microscope(SEM), and energy dispersive spectroscopy(EDS) which confirmed the selective immunointeraction of HIgG to the anti-HIgG on the electrode, thus reduced the amperometric current of [Fe(CN) 6 ] 3-/4- redox probe. The sensing electrode was placed in a designed electrochemical flow cell of SI system, where the redox probe was propelled through and the currents before and after the immunointeraction occurred were measured amperometrically by using a simple home-made amperometer. Under the optimum condition: flow rate of 2 mL min −1 , applied potential of +350 mV, [Fe(CN) 6 ] 3-/4- concentration of 10 mM and 10 min of incubation time, a linear calibration in the range of 2–100 ng mL −1 was achieved, with detection limit of 1.70 ng mL −1 . The proposed system provided good repeatability and reproducibility and the application for urine sample analysis was demonstrated.
format Journal
author Chidkamon Thunkhamrak
Preeyaporn Reanpang
Kontad Ounnunkad
Jaroon Jakmunee
author_facet Chidkamon Thunkhamrak
Preeyaporn Reanpang
Kontad Ounnunkad
Jaroon Jakmunee
author_sort Chidkamon Thunkhamrak
title Sequential injection system with amperometric immunosensor for sensitive determination of human immunoglobulin G
title_short Sequential injection system with amperometric immunosensor for sensitive determination of human immunoglobulin G
title_full Sequential injection system with amperometric immunosensor for sensitive determination of human immunoglobulin G
title_fullStr Sequential injection system with amperometric immunosensor for sensitive determination of human immunoglobulin G
title_full_unstemmed Sequential injection system with amperometric immunosensor for sensitive determination of human immunoglobulin G
title_sort sequential injection system with amperometric immunosensor for sensitive determination of human immunoglobulin g
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85018266578&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/46575
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