Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging

Leaves of Longkong which collected from Chantaburi in Thailand were extracted by the hot and cold processes using three different solvents including water, chloroform and methanol. The crude extracts were tested for antioxidative activities, tyrosinase inhibition and in vitro cytotoxicity as well as...

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Main Authors: Manosroi A., Kumguan K., Chankhampan C., Manosroi W., Manosroi J.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-84866084536&partnerID=40&md5=c7b4f38a5811c5d736c251087e4808c0
http://cmuir.cmu.ac.th/handle/6653943832/4684
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spelling th-cmuir.6653943832-46842014-08-30T02:42:44Z Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging Manosroi A. Kumguan K. Chankhampan C. Manosroi W. Manosroi J. Leaves of Longkong which collected from Chantaburi in Thailand were extracted by the hot and cold processes using three different solvents including water, chloroform and methanol. The crude extracts were tested for antioxidative activities, tyrosinase inhibition and in vitro cytotoxicity as well as the MMP-2 inhibition activity on human skin fibroblasts for anti-aging evaluation. The hot water crude extract showed the highest antioxidative activities (DPPH radical scavenging, metal ion chelating and lipid peroxidation inhibition) with the SC 50, CC 50 and IPC 50 values of 5.40±1.23, 32.31±0.84 and 3.29±0.30 mg/ml, respectively, and the highest tyrosinase inhibition activity with the IC 50 value of 0.49±0.23 mg/ml. The extract also showed no cytotoxicity on human skin fibroblasts with the cell viability of 80.52±15.16%. It demonstrated the anti-aging potential by having the pro and active MMP-2 inhibition activity, but lower than ascorbic acid of 1.28 and 1.12 times, respectively. The semi-purified extracts were prepared from this crude extract by solvent-solvent partition. The ethyl acetate soluble fraction showed higher activities (DPPH radical scavenging, metal ion chelating and tyrosinase inhibition) than the crude extract of 23.48, 71.80 and 2.58 times, respectively. This fraction exhibited similar pro and active MMP-2 inhibitory effect to the crude extract. The results from this study have indicated the possible application of the ethyl acetate fraction of the hot water crude extract from leaves of Longkong to be developed as an anti-aging product. Copyright © 2012 American Scientific Publishers All rights reserved. 2014-08-30T02:42:44Z 2014-08-30T02:42:44Z 2012 Article 15334880 10.1166/jnn.2012.6500 JNNOA http://www.scopus.com/inward/record.url?eid=2-s2.0-84866084536&partnerID=40&md5=c7b4f38a5811c5d736c251087e4808c0 http://cmuir.cmu.ac.th/handle/6653943832/4684 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Leaves of Longkong which collected from Chantaburi in Thailand were extracted by the hot and cold processes using three different solvents including water, chloroform and methanol. The crude extracts were tested for antioxidative activities, tyrosinase inhibition and in vitro cytotoxicity as well as the MMP-2 inhibition activity on human skin fibroblasts for anti-aging evaluation. The hot water crude extract showed the highest antioxidative activities (DPPH radical scavenging, metal ion chelating and lipid peroxidation inhibition) with the SC 50, CC 50 and IPC 50 values of 5.40±1.23, 32.31±0.84 and 3.29±0.30 mg/ml, respectively, and the highest tyrosinase inhibition activity with the IC 50 value of 0.49±0.23 mg/ml. The extract also showed no cytotoxicity on human skin fibroblasts with the cell viability of 80.52±15.16%. It demonstrated the anti-aging potential by having the pro and active MMP-2 inhibition activity, but lower than ascorbic acid of 1.28 and 1.12 times, respectively. The semi-purified extracts were prepared from this crude extract by solvent-solvent partition. The ethyl acetate soluble fraction showed higher activities (DPPH radical scavenging, metal ion chelating and tyrosinase inhibition) than the crude extract of 23.48, 71.80 and 2.58 times, respectively. This fraction exhibited similar pro and active MMP-2 inhibitory effect to the crude extract. The results from this study have indicated the possible application of the ethyl acetate fraction of the hot water crude extract from leaves of Longkong to be developed as an anti-aging product. Copyright © 2012 American Scientific Publishers All rights reserved.
format Article
author Manosroi A.
Kumguan K.
Chankhampan C.
Manosroi W.
Manosroi J.
spellingShingle Manosroi A.
Kumguan K.
Chankhampan C.
Manosroi W.
Manosroi J.
Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging
author_facet Manosroi A.
Kumguan K.
Chankhampan C.
Manosroi W.
Manosroi J.
author_sort Manosroi A.
title Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging
title_short Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging
title_full Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging
title_fullStr Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging
title_full_unstemmed Nanoscale gelatinase A (MMP-2) inhibition on human skin fibroblasts of Longkong (Lansium domesticum correa) Leaf extracts for anti-aging
title_sort nanoscale gelatinase a (mmp-2) inhibition on human skin fibroblasts of longkong (lansium domesticum correa) leaf extracts for anti-aging
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-84866084536&partnerID=40&md5=c7b4f38a5811c5d736c251087e4808c0
http://cmuir.cmu.ac.th/handle/6653943832/4684
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