Prevalence of centrocestus formosanus metacercariae in ornamental fish from Chiang Mai, Thailand, with molecular approach using ITS2

© 2017, Korean Society for Parasitology and Tropical Medicine. The prevalence of Centrocestus formosanus metacercariae was investigated in ornamental fish purchased from a pet shop in Chiang Mai, Thailand, including Carassius auratus (goldfish), Cyprinus carpio (Koi), Poecilia latipinna (Sailfin Mol...

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Main Authors: Atcharaphan Wanlop, Chalobol Wongsawad, Pongphol Prattapong, Pheravut Wongsawad, Thapana Chontananarth, Jong Yil Chai
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/46945
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-469452018-04-25T07:22:09Z Prevalence of centrocestus formosanus metacercariae in ornamental fish from Chiang Mai, Thailand, with molecular approach using ITS2 Atcharaphan Wanlop Chalobol Wongsawad Pongphol Prattapong Pheravut Wongsawad Thapana Chontananarth Jong Yil Chai Agricultural and Biological Sciences © 2017, Korean Society for Parasitology and Tropical Medicine. The prevalence of Centrocestus formosanus metacercariae was investigated in ornamental fish purchased from a pet shop in Chiang Mai, Thailand, including Carassius auratus (goldfish), Cyprinus carpio (Koi), Poecilia latipinna (Sailfin Molly), Danio rerio (Zebrafish), and Puntigrus tetrazona (Tiger barb). The parasite species was identified by the morphology of worms as well as by a molecular approach using ITS2. The results showed that 50 (33.3%) of 150 fish examined were infected with the metacercariae. The highest prevalence was found in C. auratus (83.3%), and the highest intensity was noted in C. carpio (70.8 metacercariae/fish). The most important morphological character was the presence of 32-34 circumoral spines on the oral sucker. The phylogenetic studies using the rRNA ITS2 region revealed that all the specimens of C. formosanus in this study were grouped together with C. formosanus in GenBank database. This is the first report on ornamental fish, C. carpio, P. latipinna, D. rerio, and P. tetrazona, taking the role of second intermediate hosts of C. formosanus in Thailand. Prevention and control of metacercarial infection in ornamental fish is urgently needed. 2018-04-25T07:05:43Z 2018-04-25T07:05:43Z 2017-08-01 Journal 17380006 00234001 2-s2.0-85029118387 10.3347/kjp.2017.55.4.445 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85029118387&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46945
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Atcharaphan Wanlop
Chalobol Wongsawad
Pongphol Prattapong
Pheravut Wongsawad
Thapana Chontananarth
Jong Yil Chai
Prevalence of centrocestus formosanus metacercariae in ornamental fish from Chiang Mai, Thailand, with molecular approach using ITS2
description © 2017, Korean Society for Parasitology and Tropical Medicine. The prevalence of Centrocestus formosanus metacercariae was investigated in ornamental fish purchased from a pet shop in Chiang Mai, Thailand, including Carassius auratus (goldfish), Cyprinus carpio (Koi), Poecilia latipinna (Sailfin Molly), Danio rerio (Zebrafish), and Puntigrus tetrazona (Tiger barb). The parasite species was identified by the morphology of worms as well as by a molecular approach using ITS2. The results showed that 50 (33.3%) of 150 fish examined were infected with the metacercariae. The highest prevalence was found in C. auratus (83.3%), and the highest intensity was noted in C. carpio (70.8 metacercariae/fish). The most important morphological character was the presence of 32-34 circumoral spines on the oral sucker. The phylogenetic studies using the rRNA ITS2 region revealed that all the specimens of C. formosanus in this study were grouped together with C. formosanus in GenBank database. This is the first report on ornamental fish, C. carpio, P. latipinna, D. rerio, and P. tetrazona, taking the role of second intermediate hosts of C. formosanus in Thailand. Prevention and control of metacercarial infection in ornamental fish is urgently needed.
format Journal
author Atcharaphan Wanlop
Chalobol Wongsawad
Pongphol Prattapong
Pheravut Wongsawad
Thapana Chontananarth
Jong Yil Chai
author_facet Atcharaphan Wanlop
Chalobol Wongsawad
Pongphol Prattapong
Pheravut Wongsawad
Thapana Chontananarth
Jong Yil Chai
author_sort Atcharaphan Wanlop
title Prevalence of centrocestus formosanus metacercariae in ornamental fish from Chiang Mai, Thailand, with molecular approach using ITS2
title_short Prevalence of centrocestus formosanus metacercariae in ornamental fish from Chiang Mai, Thailand, with molecular approach using ITS2
title_full Prevalence of centrocestus formosanus metacercariae in ornamental fish from Chiang Mai, Thailand, with molecular approach using ITS2
title_fullStr Prevalence of centrocestus formosanus metacercariae in ornamental fish from Chiang Mai, Thailand, with molecular approach using ITS2
title_full_unstemmed Prevalence of centrocestus formosanus metacercariae in ornamental fish from Chiang Mai, Thailand, with molecular approach using ITS2
title_sort prevalence of centrocestus formosanus metacercariae in ornamental fish from chiang mai, thailand, with molecular approach using its2
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85029118387&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/46945
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