Multiplex RT-PCR for rapid detection of viruses commonly causing diarrhea in pediatric patients

© 2016 Wiley Periodicals, Inc. Multiplex RT-PCR method using five sets of panel primers was developed for the detection of diarrheal viruses, including rotavirus A, B, and C, adenovirus, astrovirus, norovirus GI and GII, sapovirus, Aichi virus, parechovirus, enterovirus, cosavirus, bocavirus, and Sa...

Full description

Saved in:
Bibliographic Details
Main Authors: Aksara Thongprachum, Pattara Khamrin, Ngan Thi Kim Pham, Sayaka Takanashi, Shoko Okitsu, Hiroyuki Shimizu, Niwat Maneekarn, Satoshi Hayakawa, Hiroshi Ushijima
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85000416298&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/46952
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-46952
record_format dspace
spelling th-cmuir.6653943832-469522018-04-25T07:24:51Z Multiplex RT-PCR for rapid detection of viruses commonly causing diarrhea in pediatric patients Aksara Thongprachum Pattara Khamrin Ngan Thi Kim Pham Sayaka Takanashi Shoko Okitsu Hiroyuki Shimizu Niwat Maneekarn Satoshi Hayakawa Hiroshi Ushijima Agricultural and Biological Sciences © 2016 Wiley Periodicals, Inc. Multiplex RT-PCR method using five sets of panel primers was developed for the detection of diarrheal viruses, including rotavirus A, B, and C, adenovirus, astrovirus, norovirus GI and GII, sapovirus, Aichi virus, parechovirus, enterovirus, cosavirus, bocavirus, and Saffold virus. The sensitivity of the method was evaluated and tested with 751 fecal specimens collected from Japanese children with acute diarrhea. Several kinds of viruses were detected in 528 out of 751 (70.3%) fecal specimens. Mixed-infection with different viruses in clinical specimens could also be effectively detected. The method proved to be reliable with highly sensitive and specific and useful for routine diagnosis. J. Med. Virol. 89:818–824, 2017. © 2016 Wiley Periodicals, Inc. 2018-04-25T07:05:53Z 2018-04-25T07:05:53Z 2017-05-01 Journal 10969071 01466615 2-s2.0-85000416298 10.1002/jmv.24711 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85000416298&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46952
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Aksara Thongprachum
Pattara Khamrin
Ngan Thi Kim Pham
Sayaka Takanashi
Shoko Okitsu
Hiroyuki Shimizu
Niwat Maneekarn
Satoshi Hayakawa
Hiroshi Ushijima
Multiplex RT-PCR for rapid detection of viruses commonly causing diarrhea in pediatric patients
description © 2016 Wiley Periodicals, Inc. Multiplex RT-PCR method using five sets of panel primers was developed for the detection of diarrheal viruses, including rotavirus A, B, and C, adenovirus, astrovirus, norovirus GI and GII, sapovirus, Aichi virus, parechovirus, enterovirus, cosavirus, bocavirus, and Saffold virus. The sensitivity of the method was evaluated and tested with 751 fecal specimens collected from Japanese children with acute diarrhea. Several kinds of viruses were detected in 528 out of 751 (70.3%) fecal specimens. Mixed-infection with different viruses in clinical specimens could also be effectively detected. The method proved to be reliable with highly sensitive and specific and useful for routine diagnosis. J. Med. Virol. 89:818–824, 2017. © 2016 Wiley Periodicals, Inc.
format Journal
author Aksara Thongprachum
Pattara Khamrin
Ngan Thi Kim Pham
Sayaka Takanashi
Shoko Okitsu
Hiroyuki Shimizu
Niwat Maneekarn
Satoshi Hayakawa
Hiroshi Ushijima
author_facet Aksara Thongprachum
Pattara Khamrin
Ngan Thi Kim Pham
Sayaka Takanashi
Shoko Okitsu
Hiroyuki Shimizu
Niwat Maneekarn
Satoshi Hayakawa
Hiroshi Ushijima
author_sort Aksara Thongprachum
title Multiplex RT-PCR for rapid detection of viruses commonly causing diarrhea in pediatric patients
title_short Multiplex RT-PCR for rapid detection of viruses commonly causing diarrhea in pediatric patients
title_full Multiplex RT-PCR for rapid detection of viruses commonly causing diarrhea in pediatric patients
title_fullStr Multiplex RT-PCR for rapid detection of viruses commonly causing diarrhea in pediatric patients
title_full_unstemmed Multiplex RT-PCR for rapid detection of viruses commonly causing diarrhea in pediatric patients
title_sort multiplex rt-pcr for rapid detection of viruses commonly causing diarrhea in pediatric patients
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85000416298&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/46952
_version_ 1681422970244825088