Molecular detection and characterization of picobirnaviruses in piglets with diarrhea in Thailand
© 2016, Springer-Verlag Wien. Picobirnavirus (PBV) is a small, bi-segmented, double-stranded RNA virus. Taxonom ically, the genus Picobirnavirus belongs to the Picobirnaviridae family. PBV infects a wide range of hosts and causes opportunistic infections, but its role in diarrheal disease remains un...
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th-cmuir.6653943832-469542018-04-25T07:25:47Z Molecular detection and characterization of picobirnaviruses in piglets with diarrhea in Thailand Lauren Wilburn Arpaporn Yodmeeklin Pakawat Kochjan Wilaiporn Saikruang Kattareeya Kumthip Pattara Khamrin Niwat Maneekarn Agricultural and Biological Sciences © 2016, Springer-Verlag Wien. Picobirnavirus (PBV) is a small, bi-segmented, double-stranded RNA virus. Taxonom ically, the genus Picobirnavirus belongs to the Picobirnaviridae family. PBV infects a wide range of hosts and causes opportunistic infections, but its role in diarrheal disease remains unclear. To determine the prevalence and genetic diversity of porcine PBVs in Northern Thailand, 380 fecal samples collected from diarrheic and non-diarrheic piglets, raised in 22 pig farms, were tested for the presence of PBV. Reverse-transcription PCR (RT-PCR) was performed using primer sets specific to the RNA-dependent RNA polymerase (RdRp) gene. PBV was detected in 86 of 265 (32.5%) diarrheic piglets and in 26 of 115 (22.6%) non-diarrheic piglets. All the PBV strains detected in this study belonged to genogroup I and a high proportion of PBV-positive piglets were co-infected with group A rotavirus (RVA) and bocavirus (BoV). Phylogenetic analysis of representative genogroup I strains revealed remarkably high similarity between strains; these formed a monophyletic cluster with 97-100% sequence identity in the RdRp gene. The strains were also closely related to genogroup I PBV Chinese porcine strain. The findings indicate that PBV infection is common in piglets with and without diarrhea in Northern Thailand. 2018-04-25T07:05:54Z 2018-04-25T07:05:54Z 2017-04-01 Journal 03048608 2-s2.0-85007480771 10.1007/s00705-016-3190-3 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85007480771&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46954 |
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Agricultural and Biological Sciences Lauren Wilburn Arpaporn Yodmeeklin Pakawat Kochjan Wilaiporn Saikruang Kattareeya Kumthip Pattara Khamrin Niwat Maneekarn Molecular detection and characterization of picobirnaviruses in piglets with diarrhea in Thailand |
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© 2016, Springer-Verlag Wien. Picobirnavirus (PBV) is a small, bi-segmented, double-stranded RNA virus. Taxonom ically, the genus Picobirnavirus belongs to the Picobirnaviridae family. PBV infects a wide range of hosts and causes opportunistic infections, but its role in diarrheal disease remains unclear. To determine the prevalence and genetic diversity of porcine PBVs in Northern Thailand, 380 fecal samples collected from diarrheic and non-diarrheic piglets, raised in 22 pig farms, were tested for the presence of PBV. Reverse-transcription PCR (RT-PCR) was performed using primer sets specific to the RNA-dependent RNA polymerase (RdRp) gene. PBV was detected in 86 of 265 (32.5%) diarrheic piglets and in 26 of 115 (22.6%) non-diarrheic piglets. All the PBV strains detected in this study belonged to genogroup I and a high proportion of PBV-positive piglets were co-infected with group A rotavirus (RVA) and bocavirus (BoV). Phylogenetic analysis of representative genogroup I strains revealed remarkably high similarity between strains; these formed a monophyletic cluster with 97-100% sequence identity in the RdRp gene. The strains were also closely related to genogroup I PBV Chinese porcine strain. The findings indicate that PBV infection is common in piglets with and without diarrhea in Northern Thailand. |
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Journal |
author |
Lauren Wilburn Arpaporn Yodmeeklin Pakawat Kochjan Wilaiporn Saikruang Kattareeya Kumthip Pattara Khamrin Niwat Maneekarn |
author_facet |
Lauren Wilburn Arpaporn Yodmeeklin Pakawat Kochjan Wilaiporn Saikruang Kattareeya Kumthip Pattara Khamrin Niwat Maneekarn |
author_sort |
Lauren Wilburn |
title |
Molecular detection and characterization of picobirnaviruses in piglets with diarrhea in Thailand |
title_short |
Molecular detection and characterization of picobirnaviruses in piglets with diarrhea in Thailand |
title_full |
Molecular detection and characterization of picobirnaviruses in piglets with diarrhea in Thailand |
title_fullStr |
Molecular detection and characterization of picobirnaviruses in piglets with diarrhea in Thailand |
title_full_unstemmed |
Molecular detection and characterization of picobirnaviruses in piglets with diarrhea in Thailand |
title_sort |
molecular detection and characterization of picobirnaviruses in piglets with diarrhea in thailand |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85007480771&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/46954 |
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