Polymorphisms in the bovine HSP90AB1 gene are associated with heat tolerance in Thai indigenous cattle

Heat shock proteins act as molecular chaperones that have preferentially been transcribed in response to severe perturbations of the cellular homeostasis such as heat stress. Here the traits respiration rate (RR), rectal temperature (RT), pack cell volume (PCV) and the individual heat tolerance coef...

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Main Authors: Charoensook R., Gatphayak K., Sharifi A.R., Chaisongkram C., Brenig B., Knorr C.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-80054066149&partnerID=40&md5=113ae3cec3362c2a247af1b1ca040fbd
http://cmuir.cmu.ac.th/handle/6653943832/471
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spelling th-cmuir.6653943832-4712014-08-29T07:31:49Z Polymorphisms in the bovine HSP90AB1 gene are associated with heat tolerance in Thai indigenous cattle Charoensook R. Gatphayak K. Sharifi A.R. Chaisongkram C. Brenig B. Knorr C. Heat shock proteins act as molecular chaperones that have preferentially been transcribed in response to severe perturbations of the cellular homeostasis such as heat stress. Here the traits respiration rate (RR), rectal temperature (RT), pack cell volume (PCV) and the individual heat tolerance coefficient (HTC) were recorded as physiological responses on heat stress (environmental temperatures) in Bos taurus (crossbred Holstein Friesian; HF) and B. indicus (Thai native cattle: White Lamphun; WL and Mountain cattle; MT) animals (n = 47) in Thailand. Polymorphisms of the heat shock protein 90-kDa beta gene (HSP90AB1) were evaluated by comparative sequencing. Nine single nucleotide polymorphisms (SNP) were identified, i.e. three in exons 10 and 11, five in introns 8, 9, 10 and 11, and one in the 3′UTR. The exon 11 SNP g.5082C>T led to a missense mutation (alanine to valine). During the period of extreme heat (in the afternoon) RR and RT were elevated in each of the three breeds, whereas the PCV decreased. Mountain cattle and White Lamphun heifers recorded significantly better physiologic parameters (p < 0.05) in all traits considered, including or particularly HTC than Holstein Friesian heifers. The association analysis revealed that the T allele at SNP g.4338T>C within intron 3 improved the heat tolerance (p < 0.05). Allele T was exclusively found in White Lamphun animals and to 84% in Mountain cattle. Holstein Friesian heifers revealed an allele frequency of only 18%. Polymorphisms within HSP90AB1 were not causative for the physiological responses; however, we propose that they should at least be used as genetic markers to select appropriate breeds for hot climates. © 2011 The Author(s). 2014-08-29T07:31:49Z 2014-08-29T07:31:49Z 2011 Article in Press 494747 10.1007/s11250-011-9989-8 TAHPA http://www.scopus.com/inward/record.url?eid=2-s2.0-80054066149&partnerID=40&md5=113ae3cec3362c2a247af1b1ca040fbd http://cmuir.cmu.ac.th/handle/6653943832/471 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Heat shock proteins act as molecular chaperones that have preferentially been transcribed in response to severe perturbations of the cellular homeostasis such as heat stress. Here the traits respiration rate (RR), rectal temperature (RT), pack cell volume (PCV) and the individual heat tolerance coefficient (HTC) were recorded as physiological responses on heat stress (environmental temperatures) in Bos taurus (crossbred Holstein Friesian; HF) and B. indicus (Thai native cattle: White Lamphun; WL and Mountain cattle; MT) animals (n = 47) in Thailand. Polymorphisms of the heat shock protein 90-kDa beta gene (HSP90AB1) were evaluated by comparative sequencing. Nine single nucleotide polymorphisms (SNP) were identified, i.e. three in exons 10 and 11, five in introns 8, 9, 10 and 11, and one in the 3′UTR. The exon 11 SNP g.5082C>T led to a missense mutation (alanine to valine). During the period of extreme heat (in the afternoon) RR and RT were elevated in each of the three breeds, whereas the PCV decreased. Mountain cattle and White Lamphun heifers recorded significantly better physiologic parameters (p < 0.05) in all traits considered, including or particularly HTC than Holstein Friesian heifers. The association analysis revealed that the T allele at SNP g.4338T>C within intron 3 improved the heat tolerance (p < 0.05). Allele T was exclusively found in White Lamphun animals and to 84% in Mountain cattle. Holstein Friesian heifers revealed an allele frequency of only 18%. Polymorphisms within HSP90AB1 were not causative for the physiological responses; however, we propose that they should at least be used as genetic markers to select appropriate breeds for hot climates. © 2011 The Author(s).
format Article
author Charoensook R.
Gatphayak K.
Sharifi A.R.
Chaisongkram C.
Brenig B.
Knorr C.
spellingShingle Charoensook R.
Gatphayak K.
Sharifi A.R.
Chaisongkram C.
Brenig B.
Knorr C.
Polymorphisms in the bovine HSP90AB1 gene are associated with heat tolerance in Thai indigenous cattle
author_facet Charoensook R.
Gatphayak K.
Sharifi A.R.
Chaisongkram C.
Brenig B.
Knorr C.
author_sort Charoensook R.
title Polymorphisms in the bovine HSP90AB1 gene are associated with heat tolerance in Thai indigenous cattle
title_short Polymorphisms in the bovine HSP90AB1 gene are associated with heat tolerance in Thai indigenous cattle
title_full Polymorphisms in the bovine HSP90AB1 gene are associated with heat tolerance in Thai indigenous cattle
title_fullStr Polymorphisms in the bovine HSP90AB1 gene are associated with heat tolerance in Thai indigenous cattle
title_full_unstemmed Polymorphisms in the bovine HSP90AB1 gene are associated with heat tolerance in Thai indigenous cattle
title_sort polymorphisms in the bovine hsp90ab1 gene are associated with heat tolerance in thai indigenous cattle
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-80054066149&partnerID=40&md5=113ae3cec3362c2a247af1b1ca040fbd
http://cmuir.cmu.ac.th/handle/6653943832/471
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