Lethal and sublethal effects of a methomyl-based insecticide in Hoplobatrachus rugulosus

© 2017 The Japanese Society of Toxicologic Pathology. The aim of this study was to investigate the lethal and sublethal toxicity of a methomyl-based insecticide in Hoplobatrachus rugulosus, as methomyl-based insecticides are applied in massive amounts and agrochemicals have effects on the decline in...

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Main Authors: Waret Trachantong, Supap Saenphet, Kanokporn Saenphet, Monruedee Chaiyapo
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85010376096&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/47271
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-472712018-04-25T07:28:54Z Lethal and sublethal effects of a methomyl-based insecticide in Hoplobatrachus rugulosus Waret Trachantong Supap Saenphet Kanokporn Saenphet Monruedee Chaiyapo © 2017 The Japanese Society of Toxicologic Pathology. The aim of this study was to investigate the lethal and sublethal toxicity of a methomyl-based insecticide in Hoplobatrachus rugulosus, as methomyl-based insecticides are applied in massive amounts and agrochemicals have effects on the decline in amphibian populations. To evaluate the toxic effects of methomyl from agricultural application, a methomyl-based insecticide containing 40% methomyl was selected. The median lethal concentration of 96 hours of methomyl exposure was 8.69 ppm for H. rugulosus tadpoles. The lethal concentration also produced severe histological damage in the livers and kidneys of the exposed tadpoles. The sublethal concentration used for methomyl was 144 ppb during the metamorphosis period. It was found that the sublethal concentration of the methomyl compound could decrease growth, metamorphosis time, and size, disturb biochemical parameters, and produce histological damage. In livers, methomyl effects increased oxidative stress and dramatically decreased the glycogen level of the treated froglets. Mononuclear infiltration, blood congestion, amorphous substances, and hepatocytes vacuolization were observed throughout liver tissue. The methomyl-based insecticide also increased oxidative stress and decreased nitric oxide levels in the kidneys of the exposed froglets. Renal tissue damage including blood congestion, amorphous substances, and Bowman’s capsule spaces reduction were found in the methomyl exposure group. The methomyl compound also produced vacuoles in various stages of oocytes, but no histological damage was found in testicular tissue. Our results indicated strong toxic effects of the methomyl-based insecticide on H. rugulosus, a broadly tolerant anuran. 2018-04-25T07:28:54Z 2018-04-25T07:28:54Z 2017-01-01 Journal 09149198 2-s2.0-85010376096 10.1293/tox.2016-0039 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85010376096&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/47271
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description © 2017 The Japanese Society of Toxicologic Pathology. The aim of this study was to investigate the lethal and sublethal toxicity of a methomyl-based insecticide in Hoplobatrachus rugulosus, as methomyl-based insecticides are applied in massive amounts and agrochemicals have effects on the decline in amphibian populations. To evaluate the toxic effects of methomyl from agricultural application, a methomyl-based insecticide containing 40% methomyl was selected. The median lethal concentration of 96 hours of methomyl exposure was 8.69 ppm for H. rugulosus tadpoles. The lethal concentration also produced severe histological damage in the livers and kidneys of the exposed tadpoles. The sublethal concentration used for methomyl was 144 ppb during the metamorphosis period. It was found that the sublethal concentration of the methomyl compound could decrease growth, metamorphosis time, and size, disturb biochemical parameters, and produce histological damage. In livers, methomyl effects increased oxidative stress and dramatically decreased the glycogen level of the treated froglets. Mononuclear infiltration, blood congestion, amorphous substances, and hepatocytes vacuolization were observed throughout liver tissue. The methomyl-based insecticide also increased oxidative stress and decreased nitric oxide levels in the kidneys of the exposed froglets. Renal tissue damage including blood congestion, amorphous substances, and Bowman’s capsule spaces reduction were found in the methomyl exposure group. The methomyl compound also produced vacuoles in various stages of oocytes, but no histological damage was found in testicular tissue. Our results indicated strong toxic effects of the methomyl-based insecticide on H. rugulosus, a broadly tolerant anuran.
format Journal
author Waret Trachantong
Supap Saenphet
Kanokporn Saenphet
Monruedee Chaiyapo
spellingShingle Waret Trachantong
Supap Saenphet
Kanokporn Saenphet
Monruedee Chaiyapo
Lethal and sublethal effects of a methomyl-based insecticide in Hoplobatrachus rugulosus
author_facet Waret Trachantong
Supap Saenphet
Kanokporn Saenphet
Monruedee Chaiyapo
author_sort Waret Trachantong
title Lethal and sublethal effects of a methomyl-based insecticide in Hoplobatrachus rugulosus
title_short Lethal and sublethal effects of a methomyl-based insecticide in Hoplobatrachus rugulosus
title_full Lethal and sublethal effects of a methomyl-based insecticide in Hoplobatrachus rugulosus
title_fullStr Lethal and sublethal effects of a methomyl-based insecticide in Hoplobatrachus rugulosus
title_full_unstemmed Lethal and sublethal effects of a methomyl-based insecticide in Hoplobatrachus rugulosus
title_sort lethal and sublethal effects of a methomyl-based insecticide in hoplobatrachus rugulosus
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85010376096&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/47271
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