Effects of copolymer microstructure on the properties of electrospun poly(l -lactide-co-ε-caprolactone) absorbable nerve guide tubes

The main objective of this work has been to study the effects of copolymer microstructure, both chemical and physical, on the microporosity, in vitro hydrolytic degradability and biocompatibility of electrospun poly(l-lactide-co-ε-caprolactone), PLC, copolymer tubes for potential use as absorbable n...

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Main Authors: Boontharika Thapsukhon, Napaphat Thadavirul, Pitt Supaphol, Puttinan Meepowpan, Robert Molloy, Winita Punyodom
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84885023280&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/47366
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spelling th-cmuir.6653943832-473662018-04-25T08:39:16Z Effects of copolymer microstructure on the properties of electrospun poly(l -lactide-co-ε-caprolactone) absorbable nerve guide tubes Boontharika Thapsukhon Napaphat Thadavirul Pitt Supaphol Puttinan Meepowpan Robert Molloy Winita Punyodom The main objective of this work has been to study the effects of copolymer microstructure, both chemical and physical, on the microporosity, in vitro hydrolytic degradability and biocompatibility of electrospun poly(l-lactide-co-ε-caprolactone), PLC, copolymer tubes for potential use as absorbable nerve guides. PLC copolymers with L: C compositions of 50: 50 and 67: 33 mol % were synthesized via the ring-opening copolymerization of l-lactide (L) and ε-caprolactone (C) at 120°C for 72 h using stannous octoate (tin(II) 2-ethylhexanoate) and n-hexanol as the initiating system. Electrospinning was carried out from solution in a dichloromethane/ dimethylformamide (7: 3 v/v) mixed solvent at room temperature. The in vitro hydrolytic degradation of the electrospun PLC tubes was studied in phosphate buffer saline over a period of 36 weeks. The microporous tubes were found to be gradually degradable by a simple hydrolysis mechanism leading to random chain scission. At the end of the degradation period, the % weight retentions of the PLC 50: 50 and 67: 33 tubes were 15.6% and 70.2%, respectively. Pore stability during storage as well as cell attachment and proliferation of mouse fibroblast cells (L929) showed the greater potential of the PLC 67: 33 tubes for use as temporary scaffolds in reconstructive nerve surgery. Copyright © 2013 Wiley Periodicals, Inc. 2018-04-25T08:39:16Z 2018-04-25T08:39:16Z 2013-12-15 Journal 10974628 00218995 2-s2.0-84885023280 10.1002/app.39675 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84885023280&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/47366
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description The main objective of this work has been to study the effects of copolymer microstructure, both chemical and physical, on the microporosity, in vitro hydrolytic degradability and biocompatibility of electrospun poly(l-lactide-co-ε-caprolactone), PLC, copolymer tubes for potential use as absorbable nerve guides. PLC copolymers with L: C compositions of 50: 50 and 67: 33 mol % were synthesized via the ring-opening copolymerization of l-lactide (L) and ε-caprolactone (C) at 120°C for 72 h using stannous octoate (tin(II) 2-ethylhexanoate) and n-hexanol as the initiating system. Electrospinning was carried out from solution in a dichloromethane/ dimethylformamide (7: 3 v/v) mixed solvent at room temperature. The in vitro hydrolytic degradation of the electrospun PLC tubes was studied in phosphate buffer saline over a period of 36 weeks. The microporous tubes were found to be gradually degradable by a simple hydrolysis mechanism leading to random chain scission. At the end of the degradation period, the % weight retentions of the PLC 50: 50 and 67: 33 tubes were 15.6% and 70.2%, respectively. Pore stability during storage as well as cell attachment and proliferation of mouse fibroblast cells (L929) showed the greater potential of the PLC 67: 33 tubes for use as temporary scaffolds in reconstructive nerve surgery. Copyright © 2013 Wiley Periodicals, Inc.
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author Boontharika Thapsukhon
Napaphat Thadavirul
Pitt Supaphol
Puttinan Meepowpan
Robert Molloy
Winita Punyodom
spellingShingle Boontharika Thapsukhon
Napaphat Thadavirul
Pitt Supaphol
Puttinan Meepowpan
Robert Molloy
Winita Punyodom
Effects of copolymer microstructure on the properties of electrospun poly(l -lactide-co-ε-caprolactone) absorbable nerve guide tubes
author_facet Boontharika Thapsukhon
Napaphat Thadavirul
Pitt Supaphol
Puttinan Meepowpan
Robert Molloy
Winita Punyodom
author_sort Boontharika Thapsukhon
title Effects of copolymer microstructure on the properties of electrospun poly(l -lactide-co-ε-caprolactone) absorbable nerve guide tubes
title_short Effects of copolymer microstructure on the properties of electrospun poly(l -lactide-co-ε-caprolactone) absorbable nerve guide tubes
title_full Effects of copolymer microstructure on the properties of electrospun poly(l -lactide-co-ε-caprolactone) absorbable nerve guide tubes
title_fullStr Effects of copolymer microstructure on the properties of electrospun poly(l -lactide-co-ε-caprolactone) absorbable nerve guide tubes
title_full_unstemmed Effects of copolymer microstructure on the properties of electrospun poly(l -lactide-co-ε-caprolactone) absorbable nerve guide tubes
title_sort effects of copolymer microstructure on the properties of electrospun poly(l -lactide-co-ε-caprolactone) absorbable nerve guide tubes
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84885023280&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/47366
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