Criteria for detection of α-Thalassemia-1 Thai type deletion in routine laboratory
Background: Using a single tube multiplex real-time PCR with SYBR Greenl and high resolution melting (HRM) analysis in routine laboratory for detection of α-thalassemia-1 Southeast Asian (SEA) type deletion simultaneously with Thai type deletion is costly. Methods: Criteria for laboratory testing of...
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th-cmuir.6653943832-473922018-04-25T08:39:31Z Criteria for detection of α-Thalassemia-1 Thai type deletion in routine laboratory Sakorn Pornprasert Manoo Punyamung Kallayanee Treesuwan Background: Using a single tube multiplex real-time PCR with SYBR Greenl and high resolution melting (HRM) analysis in routine laboratory for detection of α-thalassemia-1 Southeast Asian (SEA) type deletion simultaneously with Thai type deletion is costly. Methods: Criteria for laboratory testing of α-thalassemia-1 Thai type deletion were set as follow: (1) MCV ≤ 75 fL and negative for α-thalassemia-1 SEA type deletion, β-thalassemia or homozygous HbE; (2) HbE trait with HbE level < 21.5% and negative for α-thalassemia-1 SEA type deletion; or (3) Hb Bart's and HbH found on HPLC chromatogram but negative for α-thalassemia-1 SEA type deletion. Results: From May 2010 to October 2012, the number of samples to detect for α-thalassemia-1 Thai type deletion decreased from 15,081 to 672 samples and the α-thalassemia-1 Thai type deletion trait was found in 7 samples (1.04%). Conclusions: The new testing algorithm can reduce number of samples for detection of α-thalassemia-1 Thai type deletion. 2018-04-25T08:39:31Z 2018-04-25T08:39:31Z 2013-12-01 Journal 14336510 2-s2.0-84891600436 10.7754/Clin.Lab.2013.121217 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84891600436&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/47392 |
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Background: Using a single tube multiplex real-time PCR with SYBR Greenl and high resolution melting (HRM) analysis in routine laboratory for detection of α-thalassemia-1 Southeast Asian (SEA) type deletion simultaneously with Thai type deletion is costly. Methods: Criteria for laboratory testing of α-thalassemia-1 Thai type deletion were set as follow: (1) MCV ≤ 75 fL and negative for α-thalassemia-1 SEA type deletion, β-thalassemia or homozygous HbE; (2) HbE trait with HbE level < 21.5% and negative for α-thalassemia-1 SEA type deletion; or (3) Hb Bart's and HbH found on HPLC chromatogram but negative for α-thalassemia-1 SEA type deletion. Results: From May 2010 to October 2012, the number of samples to detect for α-thalassemia-1 Thai type deletion decreased from 15,081 to 672 samples and the α-thalassemia-1 Thai type deletion trait was found in 7 samples (1.04%). Conclusions: The new testing algorithm can reduce number of samples for detection of α-thalassemia-1 Thai type deletion. |
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Journal |
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Sakorn Pornprasert Manoo Punyamung Kallayanee Treesuwan |
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Sakorn Pornprasert Manoo Punyamung Kallayanee Treesuwan Criteria for detection of α-Thalassemia-1 Thai type deletion in routine laboratory |
author_facet |
Sakorn Pornprasert Manoo Punyamung Kallayanee Treesuwan |
author_sort |
Sakorn Pornprasert |
title |
Criteria for detection of α-Thalassemia-1 Thai type deletion in routine laboratory |
title_short |
Criteria for detection of α-Thalassemia-1 Thai type deletion in routine laboratory |
title_full |
Criteria for detection of α-Thalassemia-1 Thai type deletion in routine laboratory |
title_fullStr |
Criteria for detection of α-Thalassemia-1 Thai type deletion in routine laboratory |
title_full_unstemmed |
Criteria for detection of α-Thalassemia-1 Thai type deletion in routine laboratory |
title_sort |
criteria for detection of α-thalassemia-1 thai type deletion in routine laboratory |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84891600436&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/47392 |
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