Reversed-phase liquid chromatographic-mass spectrometric determination of microcystin-LR in cyanobacteria blooms under alkaline conditions
Reversed-phase HPLC coupled to the atmospheric pressure ionization-electrospray ionization (API-ESI) MS was used for microcystin-LR detection and quantitation in samples of dried Microcystis aeruginosa cells. An alkaline linear gradient (20mmol/l ammonium hydroxide-acetonitrile, pH 9.7) was used for...
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| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2014
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| Online Access: | http://www.scopus.com/inward/record.url?eid=2-s2.0-0348047456&partnerID=40&md5=06e6adc659e15ffe6aacae2bc347ba41 http://www.ncbi.nlm.nih.gov/pubmed/14698272 http://cmuir.cmu.ac.th/handle/6653943832/4741 |
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| Institution: | Chiang Mai University |
| Language: | English |
| Summary: | Reversed-phase HPLC coupled to the atmospheric pressure ionization-electrospray ionization (API-ESI) MS was used for microcystin-LR detection and quantitation in samples of dried Microcystis aeruginosa cells. An alkaline linear gradient (20mmol/l ammonium hydroxide-acetonitrile, pH 9.7) was used for elution of the toxic peptides. Limit of detection was 1μg/ml (20ng per injection) in the scan mode of MS and 0.1μg/ml (2ng per injection) in the case of selective ion monitoring. © 2003 Elsevier B.V. All rights reserved. |
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