Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract

The flowers of African marigold (Tagetes erecta L), a medicinal plant widely cultivated in Thailand, were subjected to evaluation for total phenolics, DPPH scavenging and thiobarbituric acid-reactive substance (TBARs) assays as well as tyrosinase inhibitory activity. In preliminary studies, the ethy...

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Main Authors: Ampai Phrutivorapongkul, Kanokwan Kiattisin, Pensak Jantrawut, Sunee Chansakaow, Suwanna Vejabhikul, Pimporn Leelapornpisid
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84892577413&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/47527
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-475272018-04-25T08:41:00Z Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract Ampai Phrutivorapongkul Kanokwan Kiattisin Pensak Jantrawut Sunee Chansakaow Suwanna Vejabhikul Pimporn Leelapornpisid The flowers of African marigold (Tagetes erecta L), a medicinal plant widely cultivated in Thailand, were subjected to evaluation for total phenolics, DPPH scavenging and thiobarbituric acid-reactive substance (TBARs) assays as well as tyrosinase inhibitory activity. In preliminary studies, the ethyl acetate (EA) extract obtained by continuous extraction showed the highest activities with highest phenolic content among all extracts. Bioassay-guided fractionation of EA extract led to isolation of a flavonoid identified as quercetagetin. Interestingly, it was found that quercetagetin exhibited potent DPPH scavenging activity with IC 50 of 3.70 μg/ml which is about 2-3 times higher activity than standard quercetin (IC 50 5.07 μg/ml) and trolox (IC 50 9.93 μg/ml). Moreover, it exhibited tyrosinase inhibitory activity on L-tyrosine (IC 50 89.31 μg/ml), higher than α- and β-arbutins (IC 50 157.77 and 222.35 μg/ml) and slightly higher (IC50 128.41 μg/ml) than ellagic acid (IC 50 151.1 μg/ml) when using L-DOPA as substrate. Testing with skin fibroblasts, all the extracts and quercetagetin demonstrated no toxic effect. These finding strongly indicate that African marigold flower is a promising source of natural antioxidative and tyrosinase inhibitory substances with safe to skin. 2018-04-25T08:41:00Z 2018-04-25T08:41:00Z 2013-11-01 Journal 1011601X 2-s2.0-84892577413 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84892577413&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/47527
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description The flowers of African marigold (Tagetes erecta L), a medicinal plant widely cultivated in Thailand, were subjected to evaluation for total phenolics, DPPH scavenging and thiobarbituric acid-reactive substance (TBARs) assays as well as tyrosinase inhibitory activity. In preliminary studies, the ethyl acetate (EA) extract obtained by continuous extraction showed the highest activities with highest phenolic content among all extracts. Bioassay-guided fractionation of EA extract led to isolation of a flavonoid identified as quercetagetin. Interestingly, it was found that quercetagetin exhibited potent DPPH scavenging activity with IC 50 of 3.70 μg/ml which is about 2-3 times higher activity than standard quercetin (IC 50 5.07 μg/ml) and trolox (IC 50 9.93 μg/ml). Moreover, it exhibited tyrosinase inhibitory activity on L-tyrosine (IC 50 89.31 μg/ml), higher than α- and β-arbutins (IC 50 157.77 and 222.35 μg/ml) and slightly higher (IC50 128.41 μg/ml) than ellagic acid (IC 50 151.1 μg/ml) when using L-DOPA as substrate. Testing with skin fibroblasts, all the extracts and quercetagetin demonstrated no toxic effect. These finding strongly indicate that African marigold flower is a promising source of natural antioxidative and tyrosinase inhibitory substances with safe to skin.
format Journal
author Ampai Phrutivorapongkul
Kanokwan Kiattisin
Pensak Jantrawut
Sunee Chansakaow
Suwanna Vejabhikul
Pimporn Leelapornpisid
spellingShingle Ampai Phrutivorapongkul
Kanokwan Kiattisin
Pensak Jantrawut
Sunee Chansakaow
Suwanna Vejabhikul
Pimporn Leelapornpisid
Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract
author_facet Ampai Phrutivorapongkul
Kanokwan Kiattisin
Pensak Jantrawut
Sunee Chansakaow
Suwanna Vejabhikul
Pimporn Leelapornpisid
author_sort Ampai Phrutivorapongkul
title Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract
title_short Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract
title_full Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract
title_fullStr Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract
title_full_unstemmed Appraisal of biological activities and identification of phenolic compound of African marigold (Tagetes erecta) flower extract
title_sort appraisal of biological activities and identification of phenolic compound of african marigold (tagetes erecta) flower extract
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84892577413&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/47527
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