Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture

In this study, we focused on sericin hydrolysates, originating from silkworm used in serum-free human bone marrow-derived mesenchymal stem cells (hBM-MSCs) culture. We reported the effect of a covalent linkage between a bioactive protein molecule and polystyrene dish surface via a carbon intermediat...

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Main Authors: Somruthai Tunma, Doo Hoon Song, Si Eun Kim, Kyoung Nam Kim, Jeon Geon Han, Dheerawan Boonyawan
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84883156237&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/47578
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-475782018-04-25T08:41:36Z Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture Somruthai Tunma Doo Hoon Song Si Eun Kim Kyoung Nam Kim Jeon Geon Han Dheerawan Boonyawan In this study, we focused on sericin hydrolysates, originating from silkworm used in serum-free human bone marrow-derived mesenchymal stem cells (hBM-MSCs) culture. We reported the effect of a covalent linkage between a bioactive protein molecule and polystyrene dish surface via a carbon intermediate layer which can slow down the release rate of protein compounds into the phosphate buffer saline (PBS) solution. Films of amorphous carbon (a-C) and functionalized-carbon were deposited on PS culture dish surfaces by using a DC magnetron sputtering system and RF PECVD system. We found that a-C based-films can increase the hydrophilicity and biocompatibility of polystyrene (PS) dishes, especially a-C films and a-C:N 2 films showed good attachment of hBM-MSCs at 24 h. However, in the case of silica surface (a-C:SiO x films), the cells showed a ragged and unattached boundary resulting from the presence of surface silanol groups. For the UV-vis absorbance, all carbon modified-PS dishes showed a lower release rate of sericin molecules into PBS solution than PS control. This revealed that the functionalized carbon could be enhanced by specific binding properties with given molecules. The carbon-coated PS dishes grafting with sericin protein were used in a serum-free condition. We also found that hBM-MSCs have higher percentage of proliferated cells at day 7 for the modified dishes with carbon films and coated with sericin than the PS control coated with sericin. The physical film properties were measured by atomic force microscopy (AFM), scanning electron microscope (SEM) and contact angle measurement. The presence of NH 2 groups of sericin compounds on the PS dish was revealed by Fourier transform infrared spectroscopy (FTIR). The stability of covalent bonds of sericin molecules after washing out ungrafted sericin was confirmed by X-ray photoelectron spectroscopy (XPS). © 2013 Elsevier B.V. All rights reserved. 2018-04-25T08:41:36Z 2018-04-25T08:41:36Z 2013-10-15 Journal 01694332 2-s2.0-84883156237 10.1016/j.apsusc.2013.07.046 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84883156237&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/47578
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description In this study, we focused on sericin hydrolysates, originating from silkworm used in serum-free human bone marrow-derived mesenchymal stem cells (hBM-MSCs) culture. We reported the effect of a covalent linkage between a bioactive protein molecule and polystyrene dish surface via a carbon intermediate layer which can slow down the release rate of protein compounds into the phosphate buffer saline (PBS) solution. Films of amorphous carbon (a-C) and functionalized-carbon were deposited on PS culture dish surfaces by using a DC magnetron sputtering system and RF PECVD system. We found that a-C based-films can increase the hydrophilicity and biocompatibility of polystyrene (PS) dishes, especially a-C films and a-C:N 2 films showed good attachment of hBM-MSCs at 24 h. However, in the case of silica surface (a-C:SiO x films), the cells showed a ragged and unattached boundary resulting from the presence of surface silanol groups. For the UV-vis absorbance, all carbon modified-PS dishes showed a lower release rate of sericin molecules into PBS solution than PS control. This revealed that the functionalized carbon could be enhanced by specific binding properties with given molecules. The carbon-coated PS dishes grafting with sericin protein were used in a serum-free condition. We also found that hBM-MSCs have higher percentage of proliferated cells at day 7 for the modified dishes with carbon films and coated with sericin than the PS control coated with sericin. The physical film properties were measured by atomic force microscopy (AFM), scanning electron microscope (SEM) and contact angle measurement. The presence of NH 2 groups of sericin compounds on the PS dish was revealed by Fourier transform infrared spectroscopy (FTIR). The stability of covalent bonds of sericin molecules after washing out ungrafted sericin was confirmed by X-ray photoelectron spectroscopy (XPS). © 2013 Elsevier B.V. All rights reserved.
format Journal
author Somruthai Tunma
Doo Hoon Song
Si Eun Kim
Kyoung Nam Kim
Jeon Geon Han
Dheerawan Boonyawan
spellingShingle Somruthai Tunma
Doo Hoon Song
Si Eun Kim
Kyoung Nam Kim
Jeon Geon Han
Dheerawan Boonyawan
Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture
author_facet Somruthai Tunma
Doo Hoon Song
Si Eun Kim
Kyoung Nam Kim
Jeon Geon Han
Dheerawan Boonyawan
author_sort Somruthai Tunma
title Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture
title_short Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture
title_full Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture
title_fullStr Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture
title_full_unstemmed Immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture
title_sort immobilization of sericin molecules via amorphous carbon plasma modified-polystyrene dish for serum-free culture
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84883156237&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/47578
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