Micellar liquid chromatographic determination of sildenafil citrate in pharmaceutical formulations

A simple, rapid, precise, accurate and selective micellar liquid chromatographic (MLC) method was developed and validated for the determination of sildenafil citrate in pharmaceutical formulations. Separation was achieved isocratically on an ACE 5 C 18 - AR column (5 μm, 4.6 mm × 150 mm) utilizing...

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Main Authors: Sujaritpun Jaipang, Dammrong Santiarworn, Saisunee Liawruangrath, Boonsom Liawruangrath
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84880964200&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/47743
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-477432018-04-25T08:43:27Z Micellar liquid chromatographic determination of sildenafil citrate in pharmaceutical formulations Sujaritpun Jaipang Dammrong Santiarworn Saisunee Liawruangrath Boonsom Liawruangrath A simple, rapid, precise, accurate and selective micellar liquid chromatographic (MLC) method was developed and validated for the determination of sildenafil citrate in pharmaceutical formulations. Separation was achieved isocratically on an ACE 5 C 18 - AR column (5 μm, 4.6 mm × 150 mm) utilizing a mobile phase of 8.2 mM SDS in acetate buffer (pH 4.0) at a flow rate of 0.5 mL min -1 with UV detection at 298 nm. All analyses were conducted at ambient temperature. Linear calibration curve was obtained over the concentration range of 125-500 μg mL -1 , with a correlation coefficient (r 2 ) greater than 0.999. The limits of detection and quantitation were found to be 35 μg mL -1 and 106 μg mL -1 respectively. The proposed method has been applied to the determination of sildenafil citrate in oral suspensions and tablets. Common excipients in formulations did not interfere. This method is suitable for routine analysis. 2018-04-25T08:43:27Z 2018-04-25T08:43:27Z 2013-08-06 Journal 01252526 2-s2.0-84880964200 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84880964200&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/47743
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description A simple, rapid, precise, accurate and selective micellar liquid chromatographic (MLC) method was developed and validated for the determination of sildenafil citrate in pharmaceutical formulations. Separation was achieved isocratically on an ACE 5 C 18 - AR column (5 μm, 4.6 mm × 150 mm) utilizing a mobile phase of 8.2 mM SDS in acetate buffer (pH 4.0) at a flow rate of 0.5 mL min -1 with UV detection at 298 nm. All analyses were conducted at ambient temperature. Linear calibration curve was obtained over the concentration range of 125-500 μg mL -1 , with a correlation coefficient (r 2 ) greater than 0.999. The limits of detection and quantitation were found to be 35 μg mL -1 and 106 μg mL -1 respectively. The proposed method has been applied to the determination of sildenafil citrate in oral suspensions and tablets. Common excipients in formulations did not interfere. This method is suitable for routine analysis.
format Journal
author Sujaritpun Jaipang
Dammrong Santiarworn
Saisunee Liawruangrath
Boonsom Liawruangrath
spellingShingle Sujaritpun Jaipang
Dammrong Santiarworn
Saisunee Liawruangrath
Boonsom Liawruangrath
Micellar liquid chromatographic determination of sildenafil citrate in pharmaceutical formulations
author_facet Sujaritpun Jaipang
Dammrong Santiarworn
Saisunee Liawruangrath
Boonsom Liawruangrath
author_sort Sujaritpun Jaipang
title Micellar liquid chromatographic determination of sildenafil citrate in pharmaceutical formulations
title_short Micellar liquid chromatographic determination of sildenafil citrate in pharmaceutical formulations
title_full Micellar liquid chromatographic determination of sildenafil citrate in pharmaceutical formulations
title_fullStr Micellar liquid chromatographic determination of sildenafil citrate in pharmaceutical formulations
title_full_unstemmed Micellar liquid chromatographic determination of sildenafil citrate in pharmaceutical formulations
title_sort micellar liquid chromatographic determination of sildenafil citrate in pharmaceutical formulations
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84880964200&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/47743
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