Genomic DNA isolation from dried blood using gelatin-coated magnetic particles

Gelatin-coated magnetic particles were implemented for isolation of human genomic DNA from dried blood spotted onto glass microfibre filters. The DNA quality and quantity were compared to a silica-based membrane purification kit. Conditions for dried blood storage were varied for DNA stability deter...

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Main Authors:	Sorasak Intorasoot, Jintara Techateerawat, Amornrat Intorasoot
Format:	Journal
Published:	2018
Online Access:	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84880650274&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/47838
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Institution:	Chiang Mai University

id	th-cmuir.6653943832-47838
record_format	dspace
spelling	th-cmuir.6653943832-478382018-04-25T08:44:36Z Genomic DNA isolation from dried blood using gelatin-coated magnetic particles Sorasak Intorasoot Jintara Techateerawat Amornrat Intorasoot Gelatin-coated magnetic particles were implemented for isolation of human genomic DNA from dried blood spotted onto glass microfibre filters. The DNA quality and quantity were compared to a silica-based membrane purification kit. Conditions for dried blood storage were varied for DNA stability determination. Approximately three times higher DNA yield was recovered by coated magnetic particles than the silicabased kit. In addition, the quality of extracted DNA was determined using β-actin gene amplification by polymerase chain reaction (PCR). It was implied that genomic DNA was stable for at least 28 days in a dried condition. Gelatin-coated magnetic particles are rapid, simple and useful for DNA extraction in field applications. 2018-04-25T08:44:36Z 2018-04-25T08:44:36Z 2013-07-01 Journal 00113891 2-s2.0-84880650274 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84880650274&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/47838
institution	Chiang Mai University
building	Chiang Mai University Library
country	Thailand
collection	CMU Intellectual Repository
description	Gelatin-coated magnetic particles were implemented for isolation of human genomic DNA from dried blood spotted onto glass microfibre filters. The DNA quality and quantity were compared to a silica-based membrane purification kit. Conditions for dried blood storage were varied for DNA stability determination. Approximately three times higher DNA yield was recovered by coated magnetic particles than the silicabased kit. In addition, the quality of extracted DNA was determined using β-actin gene amplification by polymerase chain reaction (PCR). It was implied that genomic DNA was stable for at least 28 days in a dried condition. Gelatin-coated magnetic particles are rapid, simple and useful for DNA extraction in field applications.
format	Journal
author	Sorasak Intorasoot Jintara Techateerawat Amornrat Intorasoot
spellingShingle	Sorasak Intorasoot Jintara Techateerawat Amornrat Intorasoot Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
author_facet	Sorasak Intorasoot Jintara Techateerawat Amornrat Intorasoot
author_sort	Sorasak Intorasoot
title	Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
title_short	Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
title_full	Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
title_fullStr	Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
title_full_unstemmed	Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
title_sort	genomic dna isolation from dried blood using gelatin-coated magnetic particles
publishDate	2018
url	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84880650274&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/47838
_version_	1681423137289273344

Genomic DNA isolation from dried blood using gelatin-coated magnetic particles

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