Nano-ranged low-energy ion-beam-induced DNA transfer in biological cells

Low-energy ion beams at a few tens of keV were demonstrated to be able to induce exogenous macromolecules to transfer into plant and bacterial cells. In the process, the ion beam with well controlled energy and fluence bombarded living cells to cause certain degree damage in the cell envelope in nan...

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Main Authors: L. D. Yu, W. Wongkham, K. Prakrajang, K. Sangwijit, K. Inthanon, P. Thongkumkoon, P. Wanichapichart, S. Anuntalabhochai
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84877576719&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/47860
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-478602018-04-25T08:44:52Z Nano-ranged low-energy ion-beam-induced DNA transfer in biological cells L. D. Yu W. Wongkham K. Prakrajang K. Sangwijit K. Inthanon P. Thongkumkoon P. Wanichapichart S. Anuntalabhochai Low-energy ion beams at a few tens of keV were demonstrated to be able to induce exogenous macromolecules to transfer into plant and bacterial cells. In the process, the ion beam with well controlled energy and fluence bombarded living cells to cause certain degree damage in the cell envelope in nanoscales to facilitate the macromolecules such as DNA to pass through the cell envelope and enter the cell. Consequently, the technique was applied for manipulating positive improvements in the biological species. This physical DNA transfer method was highly efficient and had less risk of side-effects compared with chemical and biological methods. For better understanding of mechanisms involved in the process, a systematic study on the mechanisms was carried out. Applications of the technique were also expanded from DNA transfer in plant and bacterial cells to DNA transfection in human cancer cells potentially for the stem cell therapy purpose. Low-energy nitrogen and argon ion beams that were applied in our experiments had ranges of 100 nm or less in the cell envelope membrane which was majorly composed of polymeric cellulose. The ion beam bombardment caused chain-scission dominant damage in the polymer and electrical property changes such as increase in the impedance in the envelope membrane. These nano-modifications of the cell envelope eventually enhanced the permeability of the envelope membrane to favor the DNA transfer. The paper reports details of our research in this direction. © 2013 Elsevier B.V. 2018-04-25T08:44:52Z 2018-04-25T08:44:52Z 2013-06-15 Journal 01694332 2-s2.0-84877576719 10.1016/j.apsusc.2013.01.066 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84877576719&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/47860
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description Low-energy ion beams at a few tens of keV were demonstrated to be able to induce exogenous macromolecules to transfer into plant and bacterial cells. In the process, the ion beam with well controlled energy and fluence bombarded living cells to cause certain degree damage in the cell envelope in nanoscales to facilitate the macromolecules such as DNA to pass through the cell envelope and enter the cell. Consequently, the technique was applied for manipulating positive improvements in the biological species. This physical DNA transfer method was highly efficient and had less risk of side-effects compared with chemical and biological methods. For better understanding of mechanisms involved in the process, a systematic study on the mechanisms was carried out. Applications of the technique were also expanded from DNA transfer in plant and bacterial cells to DNA transfection in human cancer cells potentially for the stem cell therapy purpose. Low-energy nitrogen and argon ion beams that were applied in our experiments had ranges of 100 nm or less in the cell envelope membrane which was majorly composed of polymeric cellulose. The ion beam bombardment caused chain-scission dominant damage in the polymer and electrical property changes such as increase in the impedance in the envelope membrane. These nano-modifications of the cell envelope eventually enhanced the permeability of the envelope membrane to favor the DNA transfer. The paper reports details of our research in this direction. © 2013 Elsevier B.V.
format Journal
author L. D. Yu
W. Wongkham
K. Prakrajang
K. Sangwijit
K. Inthanon
P. Thongkumkoon
P. Wanichapichart
S. Anuntalabhochai
spellingShingle L. D. Yu
W. Wongkham
K. Prakrajang
K. Sangwijit
K. Inthanon
P. Thongkumkoon
P. Wanichapichart
S. Anuntalabhochai
Nano-ranged low-energy ion-beam-induced DNA transfer in biological cells
author_facet L. D. Yu
W. Wongkham
K. Prakrajang
K. Sangwijit
K. Inthanon
P. Thongkumkoon
P. Wanichapichart
S. Anuntalabhochai
author_sort L. D. Yu
title Nano-ranged low-energy ion-beam-induced DNA transfer in biological cells
title_short Nano-ranged low-energy ion-beam-induced DNA transfer in biological cells
title_full Nano-ranged low-energy ion-beam-induced DNA transfer in biological cells
title_fullStr Nano-ranged low-energy ion-beam-induced DNA transfer in biological cells
title_full_unstemmed Nano-ranged low-energy ion-beam-induced DNA transfer in biological cells
title_sort nano-ranged low-energy ion-beam-induced dna transfer in biological cells
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84877576719&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/47860
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