Sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells
A simple system for the generation of pseudoinfectious particles of dengue virus was developed to facilitate studies of virus replication and vaccine development. Selected clones of the C6/36 mosquito cell line expressing an anchored form of the dengue virus capsid protein served as host cells for t...
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th-cmuir.6653943832-479072018-04-25T08:45:24Z Sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells Sutha Sangiambut Amporn Suphatrakul Rungtawan Sriburi Poonsook Keelapang Chunya Puttikhunt Watchara Kasinrerk Prida Malasit Nopporn Sittisombut A simple system for the generation of pseudoinfectious particles of dengue virus was developed to facilitate studies of virus replication and vaccine development. Selected clones of the C6/36 mosquito cell line expressing an anchored form of the dengue virus capsid protein served as host cells for the trans-complementation of partially capsid-deleted viral RNA generated in vitro. Transfection of the partially capsid-deleted viral RNA into the anchored capsid-expressing C6/36 cells resulted in moderate titers of infectious virus. Progeny viruses multiplied in the capsid trans-complementing C6/36 cells for up to three weeks, but only initiated single rounds of replication in Vero cells lacking the capsid protein. Employing this trans-complementation system, it was found that nearly all of the capsid-coding sequence in the viral RNA was dispensable for the generation of pseudoinfectious dengue virus particles in mosquito cells. © 2013 Elsevier B.V. 2018-04-25T08:45:24Z 2018-04-25T08:45:24Z 2013-06-01 Journal 18727492 01681702 2-s2.0-84877624305 10.1016/j.virusres.2013.02.009 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84877624305&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/47907 |
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A simple system for the generation of pseudoinfectious particles of dengue virus was developed to facilitate studies of virus replication and vaccine development. Selected clones of the C6/36 mosquito cell line expressing an anchored form of the dengue virus capsid protein served as host cells for the trans-complementation of partially capsid-deleted viral RNA generated in vitro. Transfection of the partially capsid-deleted viral RNA into the anchored capsid-expressing C6/36 cells resulted in moderate titers of infectious virus. Progeny viruses multiplied in the capsid trans-complementing C6/36 cells for up to three weeks, but only initiated single rounds of replication in Vero cells lacking the capsid protein. Employing this trans-complementation system, it was found that nearly all of the capsid-coding sequence in the viral RNA was dispensable for the generation of pseudoinfectious dengue virus particles in mosquito cells. © 2013 Elsevier B.V. |
format |
Journal |
author |
Sutha Sangiambut Amporn Suphatrakul Rungtawan Sriburi Poonsook Keelapang Chunya Puttikhunt Watchara Kasinrerk Prida Malasit Nopporn Sittisombut |
spellingShingle |
Sutha Sangiambut Amporn Suphatrakul Rungtawan Sriburi Poonsook Keelapang Chunya Puttikhunt Watchara Kasinrerk Prida Malasit Nopporn Sittisombut Sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells |
author_facet |
Sutha Sangiambut Amporn Suphatrakul Rungtawan Sriburi Poonsook Keelapang Chunya Puttikhunt Watchara Kasinrerk Prida Malasit Nopporn Sittisombut |
author_sort |
Sutha Sangiambut |
title |
Sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells |
title_short |
Sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells |
title_full |
Sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells |
title_fullStr |
Sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells |
title_full_unstemmed |
Sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells |
title_sort |
sustained replication of dengue pseudoinfectious virus lacking the capsid gene by trans-complementation in capsid-producing mosquito cells |
publishDate |
2018 |
url |
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84877624305&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/47907 |
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1681423150392279040 |