Detection of coinherited Hb H-constant spring/Paksé disease and Hb e by capillary electrophoresis and high performance liquid chromatography

A capillary electrophoresis (CE) method has been proven to be superior to a high performance liquid chromatography (HPLC) method in the detection of Hb H-Constant Spring/Paksé [Hb H (β4) Hb CS or α142, Term→Gln, TAA > CAA (α2)]/Paksé [α142, Term→Tyr, TAA > TAT (α2)]. It also has the ability to...

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Main Authors: Sakorn Pornprasert, Jarurin Waneesorn
Format: Journal
Published: 2018
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84874901442&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/48055
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spelling th-cmuir.6653943832-480552018-04-25T08:47:11Z Detection of coinherited Hb H-constant spring/Paksé disease and Hb e by capillary electrophoresis and high performance liquid chromatography Sakorn Pornprasert Jarurin Waneesorn A capillary electrophoresis (CE) method has been proven to be superior to a high performance liquid chromatography (HPLC) method in the detection of Hb H-Constant Spring/Paksé [Hb H (β4) Hb CS or α142, Term→Gln, TAA > CAA (α2)]/Paksé [α142, Term→Tyr, TAA > TAT (α2)]. It also has the ability to quantify Hb Bart's (γ4). The aim of this study is to analyze the efficacy of the CE and HPLC in the detection of Hb H (β4)-CS/Paksé-E [β26(B8)Glu→Lys, GAG > AAG] disease. The laboratory results from July 2009 to July 2012 were reviewed at the Thalassemia Laboratory of the Associated Medical Sciences Clinical Service Center, Chiang-Mai University, Chiang-Mai, Thailand. The HPLC or CE method was used for the diagnosis of β-thalassemia (β-thal) and hemoglobinopathies, and molecular analysis was used for the diagnosis of α-thalassemia-1 (α-thal-1) Southeast Asian (SEA) and Thai type deletions, Hb CS and Hb Paksé. Hb H-CS-E was found in six samples and Hb H-Paksé-E was found in one sample, respectively. On the capillary electrophoregram, peaks of Hb Bart's and Hb CS/Paksé were observed in all samples with the mean levels at 2.4 and 1.0%, respectively. These peaks were also presented on the HPLC chromatogram. However, the Hb CS/Paksé level could be quantified in only three of these seven (43.0%) samples. Therefore, CE was proven to be superior to HPLC in the detection of Hb H-CS/Paksé-E disease, which will assist in diagnostic, counseling and prevention programs for these diseases. © 2013 Informa Healthcare USA, Inc. 2018-04-25T08:47:11Z 2018-04-25T08:47:11Z 2013-03-15 Journal 1532432X 03630269 2-s2.0-84874901442 10.3109/03630269.2012.752744 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84874901442&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/48055
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
description A capillary electrophoresis (CE) method has been proven to be superior to a high performance liquid chromatography (HPLC) method in the detection of Hb H-Constant Spring/Paksé [Hb H (β4) Hb CS or α142, Term→Gln, TAA > CAA (α2)]/Paksé [α142, Term→Tyr, TAA > TAT (α2)]. It also has the ability to quantify Hb Bart's (γ4). The aim of this study is to analyze the efficacy of the CE and HPLC in the detection of Hb H (β4)-CS/Paksé-E [β26(B8)Glu→Lys, GAG > AAG] disease. The laboratory results from July 2009 to July 2012 were reviewed at the Thalassemia Laboratory of the Associated Medical Sciences Clinical Service Center, Chiang-Mai University, Chiang-Mai, Thailand. The HPLC or CE method was used for the diagnosis of β-thalassemia (β-thal) and hemoglobinopathies, and molecular analysis was used for the diagnosis of α-thalassemia-1 (α-thal-1) Southeast Asian (SEA) and Thai type deletions, Hb CS and Hb Paksé. Hb H-CS-E was found in six samples and Hb H-Paksé-E was found in one sample, respectively. On the capillary electrophoregram, peaks of Hb Bart's and Hb CS/Paksé were observed in all samples with the mean levels at 2.4 and 1.0%, respectively. These peaks were also presented on the HPLC chromatogram. However, the Hb CS/Paksé level could be quantified in only three of these seven (43.0%) samples. Therefore, CE was proven to be superior to HPLC in the detection of Hb H-CS/Paksé-E disease, which will assist in diagnostic, counseling and prevention programs for these diseases. © 2013 Informa Healthcare USA, Inc.
format Journal
author Sakorn Pornprasert
Jarurin Waneesorn
spellingShingle Sakorn Pornprasert
Jarurin Waneesorn
Detection of coinherited Hb H-constant spring/Paksé disease and Hb e by capillary electrophoresis and high performance liquid chromatography
author_facet Sakorn Pornprasert
Jarurin Waneesorn
author_sort Sakorn Pornprasert
title Detection of coinherited Hb H-constant spring/Paksé disease and Hb e by capillary electrophoresis and high performance liquid chromatography
title_short Detection of coinherited Hb H-constant spring/Paksé disease and Hb e by capillary electrophoresis and high performance liquid chromatography
title_full Detection of coinherited Hb H-constant spring/Paksé disease and Hb e by capillary electrophoresis and high performance liquid chromatography
title_fullStr Detection of coinherited Hb H-constant spring/Paksé disease and Hb e by capillary electrophoresis and high performance liquid chromatography
title_full_unstemmed Detection of coinherited Hb H-constant spring/Paksé disease and Hb e by capillary electrophoresis and high performance liquid chromatography
title_sort detection of coinherited hb h-constant spring/paksé disease and hb e by capillary electrophoresis and high performance liquid chromatography
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84874901442&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/48055
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