Hypoglycemic activity of the Anisopus mannii N. E. Br. methanolic leaf extract in normal and alloxan-induced diabetic mice
The hypoglycemic activities of nine sub-fractions from the methanolic leaf crude extract of Anisopus mannii were investigated in normoglycemic and alloxan-induced diabetic mice. The methanolic sub-fraction M at 400 mg/kg bw showed significantly (p < 0.05) high reduction in fasting blood glucose (...
Saved in:
Main Authors: | , , , , , |
---|---|
Format: | Journal |
Published: |
2018
|
Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85003322402&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/48210 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Chiang Mai University |
id |
th-cmuir.6653943832-48210 |
---|---|
record_format |
dspace |
spelling |
th-cmuir.6653943832-482102018-04-25T08:49:09Z Hypoglycemic activity of the Anisopus mannii N. E. Br. methanolic leaf extract in normal and alloxan-induced diabetic mice Moses Z. Zaruwa Aranya Manosroi Toshihiro Akihisa Worapaka Manosroi Samreung Rangdaeng Jiradej Manosroi The hypoglycemic activities of nine sub-fractions from the methanolic leaf crude extract of Anisopus mannii were investigated in normoglycemic and alloxan-induced diabetic mice. The methanolic sub-fraction M at 400 mg/kg bw showed significantly (p < 0.05) high reduction in fasting blood glucose (FBG) at 27.36 and 65.57% in normoglycemic and diabetic mice, respectively. In acute toxicity test, M at 2,000 and 5,000 mg/kg bw showed reduction in blood urea nitrogen and creatinine level, elevations in aspartate transaminase, alanine transaminase and total bilirubin levels, as well as the body weights. The weight-ratios of kidney and liver to the body weight of the mice fed with these doses of M were reduced with no sign of histopathological alteration. The M at 250 mg/kg bw significantly reduced the FBG levels in a postprandial study. The hypoglycemic effect of M was eliminated when co-administered with isosorbide dinitrate or nifedipine indicating the induction of insulin secretion via K+ ATP-dependent channels. The UV/HPLC analysis of M indicated saponin at 7.7 mg/g. This study confirmed the traditional use of A. mannii for diabetes mellitus and the potential for the further development as a novel hypoglycemic drug. 2018-04-25T08:49:09Z 2018-04-25T08:49:09Z 2013-01-01 Journal 15533840 2-s2.0-85003322402 10.1515/jcim-2012-0037 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85003322402&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/48210 |
institution |
Chiang Mai University |
building |
Chiang Mai University Library |
country |
Thailand |
collection |
CMU Intellectual Repository |
description |
The hypoglycemic activities of nine sub-fractions from the methanolic leaf crude extract of Anisopus mannii were investigated in normoglycemic and alloxan-induced diabetic mice. The methanolic sub-fraction M at 400 mg/kg bw showed significantly (p < 0.05) high reduction in fasting blood glucose (FBG) at 27.36 and 65.57% in normoglycemic and diabetic mice, respectively. In acute toxicity test, M at 2,000 and 5,000 mg/kg bw showed reduction in blood urea nitrogen and creatinine level, elevations in aspartate transaminase, alanine transaminase and total bilirubin levels, as well as the body weights. The weight-ratios of kidney and liver to the body weight of the mice fed with these doses of M were reduced with no sign of histopathological alteration. The M at 250 mg/kg bw significantly reduced the FBG levels in a postprandial study. The hypoglycemic effect of M was eliminated when co-administered with isosorbide dinitrate or nifedipine indicating the induction of insulin secretion via K+ ATP-dependent channels. The UV/HPLC analysis of M indicated saponin at 7.7 mg/g. This study confirmed the traditional use of A. mannii for diabetes mellitus and the potential for the further development as a novel hypoglycemic drug. |
format |
Journal |
author |
Moses Z. Zaruwa Aranya Manosroi Toshihiro Akihisa Worapaka Manosroi Samreung Rangdaeng Jiradej Manosroi |
spellingShingle |
Moses Z. Zaruwa Aranya Manosroi Toshihiro Akihisa Worapaka Manosroi Samreung Rangdaeng Jiradej Manosroi Hypoglycemic activity of the Anisopus mannii N. E. Br. methanolic leaf extract in normal and alloxan-induced diabetic mice |
author_facet |
Moses Z. Zaruwa Aranya Manosroi Toshihiro Akihisa Worapaka Manosroi Samreung Rangdaeng Jiradej Manosroi |
author_sort |
Moses Z. Zaruwa |
title |
Hypoglycemic activity of the Anisopus mannii N. E. Br. methanolic leaf extract in normal and alloxan-induced diabetic mice |
title_short |
Hypoglycemic activity of the Anisopus mannii N. E. Br. methanolic leaf extract in normal and alloxan-induced diabetic mice |
title_full |
Hypoglycemic activity of the Anisopus mannii N. E. Br. methanolic leaf extract in normal and alloxan-induced diabetic mice |
title_fullStr |
Hypoglycemic activity of the Anisopus mannii N. E. Br. methanolic leaf extract in normal and alloxan-induced diabetic mice |
title_full_unstemmed |
Hypoglycemic activity of the Anisopus mannii N. E. Br. methanolic leaf extract in normal and alloxan-induced diabetic mice |
title_sort |
hypoglycemic activity of the anisopus mannii n. e. br. methanolic leaf extract in normal and alloxan-induced diabetic mice |
publishDate |
2018 |
url |
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85003322402&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/48210 |
_version_ |
1681423207146455040 |