Protective effects of Lactococcus chungangensis CAU 28 on alcohol-metabolizing enzyme activity in rats

© 2018 American Dairy Science Association In this study, we investigated the beneficial effects of Lactococcus chungangensis CAU 28, a bacterial strain of nondairy origin, on alcohol metabolism in rats treated with ethanol, focusing on alcohol elimination and prevention of damage and comparing the e...

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Main Authors: Maytiya Konkit, Kiyoung Kim, Jong Hwa Kim, Wonyong Kim
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/48705
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spelling th-cmuir.6653943832-487052018-06-18T08:57:21Z Protective effects of Lactococcus chungangensis CAU 28 on alcohol-metabolizing enzyme activity in rats Maytiya Konkit Kiyoung Kim Jong Hwa Kim Wonyong Kim Agricultural and Biological Sciences © 2018 American Dairy Science Association In this study, we investigated the beneficial effects of Lactococcus chungangensis CAU 28, a bacterial strain of nondairy origin, on alcohol metabolism in rats treated with ethanol, focusing on alcohol elimination and prevention of damage and comparing the effects with those observed for Lactococcus lactis ssp. lactis ATCC 19435. Male Sprague-Dawley rats were orally administered 20% ethanol and 3 substrates (freeze-dried cells, cream cheese, and yogurt) containing Lc. chungangensis CAU 28 or Lc. lactis ssp. lactis ATCC 19435, which were provided 1 h before or 1 h after ethanol ingestion. Blood samples were collected from the tail veins of the rats at 1, 3, 6, 12, and 24 h after ingestion of ethanol, Lc. chungangensis CAU 28 substrate, or Lc. lactis ssp. lactis ATCC 19435 substrate. Alcohol and acetaldehyde concentrations in the Lc. chungangensis CAU 28 substrate-treated rats were significantly reduced in a time-dependent manner compared with those in the Lc. lactis ssp. lactis ATCC 19435 substrate-treated rats. Among the experimental groups, treatment with cream cheese before ingestion of 20% ethanol was found to be the most effective method for reducing both alcohol and acetaldehyde levels in the blood. Alanine aminotransferase and aspartate aminotransferase activities in the Lc. chungangensis CAU 28 substrate-treated rats were significantly lower than those in the positive controls. Moreover, in the Lc. chungangensis CAU 28 cream cheese-treated group, rats showed a reduction of liver enzymes by up to 60%, with good effectiveness observed for both pre- and post-ethanol ingestion. These results suggested that intake of lactic acid bacteria, particularly in Lc. chungangensis CAU 28-supplemented dairy products, may reduce blood alcohol and acetaldehyde concentrations, thereby mitigating acute alcohol-induced hepatotoxicity by altering alcohol-metabolizing enzyme activities. 2018-06-18T08:57:21Z 2018-06-18T08:57:21Z 2018-01-01 Journal 15253198 00220302 2-s2.0-85045879117 10.3168/jds.2017-13992 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85045879117&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/48705
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Maytiya Konkit
Kiyoung Kim
Jong Hwa Kim
Wonyong Kim
Protective effects of Lactococcus chungangensis CAU 28 on alcohol-metabolizing enzyme activity in rats
description © 2018 American Dairy Science Association In this study, we investigated the beneficial effects of Lactococcus chungangensis CAU 28, a bacterial strain of nondairy origin, on alcohol metabolism in rats treated with ethanol, focusing on alcohol elimination and prevention of damage and comparing the effects with those observed for Lactococcus lactis ssp. lactis ATCC 19435. Male Sprague-Dawley rats were orally administered 20% ethanol and 3 substrates (freeze-dried cells, cream cheese, and yogurt) containing Lc. chungangensis CAU 28 or Lc. lactis ssp. lactis ATCC 19435, which were provided 1 h before or 1 h after ethanol ingestion. Blood samples were collected from the tail veins of the rats at 1, 3, 6, 12, and 24 h after ingestion of ethanol, Lc. chungangensis CAU 28 substrate, or Lc. lactis ssp. lactis ATCC 19435 substrate. Alcohol and acetaldehyde concentrations in the Lc. chungangensis CAU 28 substrate-treated rats were significantly reduced in a time-dependent manner compared with those in the Lc. lactis ssp. lactis ATCC 19435 substrate-treated rats. Among the experimental groups, treatment with cream cheese before ingestion of 20% ethanol was found to be the most effective method for reducing both alcohol and acetaldehyde levels in the blood. Alanine aminotransferase and aspartate aminotransferase activities in the Lc. chungangensis CAU 28 substrate-treated rats were significantly lower than those in the positive controls. Moreover, in the Lc. chungangensis CAU 28 cream cheese-treated group, rats showed a reduction of liver enzymes by up to 60%, with good effectiveness observed for both pre- and post-ethanol ingestion. These results suggested that intake of lactic acid bacteria, particularly in Lc. chungangensis CAU 28-supplemented dairy products, may reduce blood alcohol and acetaldehyde concentrations, thereby mitigating acute alcohol-induced hepatotoxicity by altering alcohol-metabolizing enzyme activities.
format Journal
author Maytiya Konkit
Kiyoung Kim
Jong Hwa Kim
Wonyong Kim
author_facet Maytiya Konkit
Kiyoung Kim
Jong Hwa Kim
Wonyong Kim
author_sort Maytiya Konkit
title Protective effects of Lactococcus chungangensis CAU 28 on alcohol-metabolizing enzyme activity in rats
title_short Protective effects of Lactococcus chungangensis CAU 28 on alcohol-metabolizing enzyme activity in rats
title_full Protective effects of Lactococcus chungangensis CAU 28 on alcohol-metabolizing enzyme activity in rats
title_fullStr Protective effects of Lactococcus chungangensis CAU 28 on alcohol-metabolizing enzyme activity in rats
title_full_unstemmed Protective effects of Lactococcus chungangensis CAU 28 on alcohol-metabolizing enzyme activity in rats
title_sort protective effects of lactococcus chungangensis cau 28 on alcohol-metabolizing enzyme activity in rats
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85045879117&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/48705
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