Thermal behaviour and tolerance to ionic liquid [emim]OAc in GH10 xylanase from Thermoascus aurantiacus SL16W

• Main Authors: Chawachart N., Anbarasan S., Turunen S., Li H., Khanongnuch C., Hummel M., Sixta H., Granstrom T., Lumyong S., Turunen O.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-84904845284&partnerID=40&md5=075317d1574a2dfb7226b3510db6fc00; http://cmuir.cmu.ac.th/handle/6653943832/4884
• Institution: Chiang Mai University

GH10 xylanase from Thermoascus aurantiacus strain SL16W (TasXyn10A) showed high stability and activity up to 70-75 °C. The enzyme's half-lives were 101 h, 65 h, 63 min and 6 min at 60, 70, 75 and 80 °C, respectively. The melting point (Tm), as measured by DSC, was 78.5 °C, which is in line with...