Pythium insidiosum Thai isolates: Molecular phylogenetic analysis

Background: Pythium insidiosum is an oomycete that infects both humans and animals, leading to a lifethreatening infectious disease called "pythiosis". Animal pythiosis presents with lesions of the skin, gastrointestinal tract, lung and bone, whereas human pythiosis presents with two commo...

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Main Authors: Angkana Chaiprasert, Theerapong Krajaejun, Srisuda Pannanusorn, Chularut Prariyachatigul, Wanchai Wanachiwanawin, Boonmee Sathapatayavongs, Tada Juthayothin, Nat Smittipat, Nongnuch Vanittanakom, Ariya Chindamporn
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/48842
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-488422018-08-16T02:13:50Z Pythium insidiosum Thai isolates: Molecular phylogenetic analysis Angkana Chaiprasert Theerapong Krajaejun Srisuda Pannanusorn Chularut Prariyachatigul Wanchai Wanachiwanawin Boonmee Sathapatayavongs Tada Juthayothin Nat Smittipat Nongnuch Vanittanakom Ariya Chindamporn Biochemistry, Genetics and Molecular Biology Medicine Background: Pythium insidiosum is an oomycete that infects both humans and animals, leading to a lifethreatening infectious disease called "pythiosis". Animal pythiosis presents with lesions of the skin, gastrointestinal tract, lung and bone, whereas human pythiosis presents with two common clinical forms, vascular pythiosis involving arteries, and ocular pythiosis involving the eye. Pythiosis in humans has been reported exclusively from Thailand. The disease in animals has been found around the world, but its occurrence has never been reported from Thailand. Objective: To group P. insidiosum based on molecular phylogenetic analysis, investigating correlation between phylogenetic group, geographic distribution, and host specificity of this pathogen. Methods: 113 rDNA internal transcribed spacer sequences of P. insidiosum were also obtained for phylogenetic analyses. These included 32 human isolates and 59 environmental isolates from Thailand, and four additional human isolates and 18 animal isolates from around the world. Results: P. insidiosum existed in three distinct clades in accordance with geographic distribution; clade-I contained American isolates, clade-II contained Asian and Australian isolates, and clade-III contained mainly Thai isolates. The Thai isolates existed only in clade-II and clade-III. Conclusion: There were two major subpopulations of P. insidiosum in Thailand. There were no correlation between the two Thai subpopulations of P. insidiosum and geographic regions or host specificity. 2018-08-16T02:05:42Z 2018-08-16T02:05:42Z 2009-12-01 Journal 19057415 2-s2.0-77955303055 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77955303055&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/48842
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Angkana Chaiprasert
Theerapong Krajaejun
Srisuda Pannanusorn
Chularut Prariyachatigul
Wanchai Wanachiwanawin
Boonmee Sathapatayavongs
Tada Juthayothin
Nat Smittipat
Nongnuch Vanittanakom
Ariya Chindamporn
Pythium insidiosum Thai isolates: Molecular phylogenetic analysis
description Background: Pythium insidiosum is an oomycete that infects both humans and animals, leading to a lifethreatening infectious disease called "pythiosis". Animal pythiosis presents with lesions of the skin, gastrointestinal tract, lung and bone, whereas human pythiosis presents with two common clinical forms, vascular pythiosis involving arteries, and ocular pythiosis involving the eye. Pythiosis in humans has been reported exclusively from Thailand. The disease in animals has been found around the world, but its occurrence has never been reported from Thailand. Objective: To group P. insidiosum based on molecular phylogenetic analysis, investigating correlation between phylogenetic group, geographic distribution, and host specificity of this pathogen. Methods: 113 rDNA internal transcribed spacer sequences of P. insidiosum were also obtained for phylogenetic analyses. These included 32 human isolates and 59 environmental isolates from Thailand, and four additional human isolates and 18 animal isolates from around the world. Results: P. insidiosum existed in three distinct clades in accordance with geographic distribution; clade-I contained American isolates, clade-II contained Asian and Australian isolates, and clade-III contained mainly Thai isolates. The Thai isolates existed only in clade-II and clade-III. Conclusion: There were two major subpopulations of P. insidiosum in Thailand. There were no correlation between the two Thai subpopulations of P. insidiosum and geographic regions or host specificity.
format Journal
author Angkana Chaiprasert
Theerapong Krajaejun
Srisuda Pannanusorn
Chularut Prariyachatigul
Wanchai Wanachiwanawin
Boonmee Sathapatayavongs
Tada Juthayothin
Nat Smittipat
Nongnuch Vanittanakom
Ariya Chindamporn
author_facet Angkana Chaiprasert
Theerapong Krajaejun
Srisuda Pannanusorn
Chularut Prariyachatigul
Wanchai Wanachiwanawin
Boonmee Sathapatayavongs
Tada Juthayothin
Nat Smittipat
Nongnuch Vanittanakom
Ariya Chindamporn
author_sort Angkana Chaiprasert
title Pythium insidiosum Thai isolates: Molecular phylogenetic analysis
title_short Pythium insidiosum Thai isolates: Molecular phylogenetic analysis
title_full Pythium insidiosum Thai isolates: Molecular phylogenetic analysis
title_fullStr Pythium insidiosum Thai isolates: Molecular phylogenetic analysis
title_full_unstemmed Pythium insidiosum Thai isolates: Molecular phylogenetic analysis
title_sort pythium insidiosum thai isolates: molecular phylogenetic analysis
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77955303055&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/48842
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