Development of immunochromatographic assay for the on-site detection of salbutamol
Salbutamol, one of the β-agonists, is misused as a growth promoter in meat producing animals. In-house synthesized colloidal gold was conjugated with the polyclonal anti-salbutamol antibodies. A rapid immunochromatographic assay was developed in a competitive format. The salbutamol-BSA conjugate and...
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th-cmuir.6653943832-488462018-08-16T02:14:04Z Development of immunochromatographic assay for the on-site detection of salbutamol Yaowapa Khamta Mookda Pattarawarapan Sawitree Nangola Chatchai Tayapiwatana Biochemistry, Genetics and Molecular Biology Health Professions Immunology and Microbiology Medicine Salbutamol, one of the β-agonists, is misused as a growth promoter in meat producing animals. In-house synthesized colloidal gold was conjugated with the polyclonal anti-salbutamol antibodies. A rapid immunochromatographic assay was developed in a competitive format. The salbutamol-BSA conjugate and goat anti-rabbit IgG were immobilized on a nitrocellulose membrane as test and control lines, respectively. The color intensity of a purple test line was inversely proportional to the amount of salbutamol presenting in the samples. The sensitivity was estimated to be about 80ng/mL of salbutamol in PBS. The method can be useful as an on-site screening procedure for detection of salbutamol. 2018-08-16T02:05:46Z 2018-08-16T02:05:46Z 2009-10-01 Journal 15324230 15321819 2-s2.0-70449730062 10.1080/15321810903188284 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70449730062&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/48846 |
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Biochemistry, Genetics and Molecular Biology Health Professions Immunology and Microbiology Medicine |
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Biochemistry, Genetics and Molecular Biology Health Professions Immunology and Microbiology Medicine Yaowapa Khamta Mookda Pattarawarapan Sawitree Nangola Chatchai Tayapiwatana Development of immunochromatographic assay for the on-site detection of salbutamol |
| description |
Salbutamol, one of the β-agonists, is misused as a growth promoter in meat producing animals. In-house synthesized colloidal gold was conjugated with the polyclonal anti-salbutamol antibodies. A rapid immunochromatographic assay was developed in a competitive format. The salbutamol-BSA conjugate and goat anti-rabbit IgG were immobilized on a nitrocellulose membrane as test and control lines, respectively. The color intensity of a purple test line was inversely proportional to the amount of salbutamol presenting in the samples. The sensitivity was estimated to be about 80ng/mL of salbutamol in PBS. The method can be useful as an on-site screening procedure for detection of salbutamol. |
| format |
Journal |
| author |
Yaowapa Khamta Mookda Pattarawarapan Sawitree Nangola Chatchai Tayapiwatana |
| author_facet |
Yaowapa Khamta Mookda Pattarawarapan Sawitree Nangola Chatchai Tayapiwatana |
| author_sort |
Yaowapa Khamta |
| title |
Development of immunochromatographic assay for the on-site detection of salbutamol |
| title_short |
Development of immunochromatographic assay for the on-site detection of salbutamol |
| title_full |
Development of immunochromatographic assay for the on-site detection of salbutamol |
| title_fullStr |
Development of immunochromatographic assay for the on-site detection of salbutamol |
| title_full_unstemmed |
Development of immunochromatographic assay for the on-site detection of salbutamol |
| title_sort |
development of immunochromatographic assay for the on-site detection of salbutamol |
| publishDate |
2018 |
| url |
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70449730062&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/48846 |
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1681423303573504000 |