The TviA auxiliary protein renders the Satmonella enterica serotype Typhi RcsB regulon responsive to changes in osmolarity

In response to osmolarity, Salmonella enterica serotype Typhi (S. Typhi) regulates genes required for Vi capsular antigen expression oppositely to those required for motility and invasion. Previous studies suggest that osmoregulation of motility, invasion and capsule expression is mediated through t...

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Main Authors: Sebastian E. Winter, Maria G. Winter, Parameth Thiennimitr, Valerie A. Gerriets, Sean Paul Nuccio, Holger Rüssmann, Andreas J. Bäumler
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/48847
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-488472018-08-16T02:10:39Z The TviA auxiliary protein renders the Satmonella enterica serotype Typhi RcsB regulon responsive to changes in osmolarity Sebastian E. Winter Maria G. Winter Parameth Thiennimitr Valerie A. Gerriets Sean Paul Nuccio Holger Rüssmann Andreas J. Bäumler Biochemistry, Genetics and Molecular Biology Immunology and Microbiology In response to osmolarity, Salmonella enterica serotype Typhi (S. Typhi) regulates genes required for Vi capsular antigen expression oppositely to those required for motility and invasion. Previous studies suggest that osmoregulation of motility, invasion and capsule expression is mediated through the RcsC/ RcsD/RcsB phosphorelay system. Here we performed gene expression profiling and functional studies to determine the role of TviA, an auxiliary protein of the RcsB response regulator, in controlling virulence gene expression in S. Typhi. TviA repressed expression of genes encoding flagella and the invasionassociated type III secretion system (T3SS-1) through repression of the flagellar regulators flhDC and fliZ, resulting in reduced invasion, reduced motility and reduced expression of FliC. Both RcsB and TviA repressed expression of flhDC, but only TviA altered flhDC expression in response to osmolarity. Introduction of tviA into S. enterica serotype Typhimurium rendered flhDC transcription sensitive to changes in osmolarity. These data suggest that the auxiliary TviA protein integrates a new regulatory input into the RcsB regulon of S. Typhi, thereby altering expression of genes encoding flagella, the Vi antigen and T3SS-1 in response to osmolarity. © 2009 Blackwell Publishing Ltd. 2018-08-16T02:05:46Z 2018-08-16T02:05:46Z 2009-10-01 Journal 13652958 0950382X 2-s2.0-70350169211 10.1111/j.1365-2958.2009.06859.x https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70350169211&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/48847
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
spellingShingle Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Sebastian E. Winter
Maria G. Winter
Parameth Thiennimitr
Valerie A. Gerriets
Sean Paul Nuccio
Holger Rüssmann
Andreas J. Bäumler
The TviA auxiliary protein renders the Satmonella enterica serotype Typhi RcsB regulon responsive to changes in osmolarity
description In response to osmolarity, Salmonella enterica serotype Typhi (S. Typhi) regulates genes required for Vi capsular antigen expression oppositely to those required for motility and invasion. Previous studies suggest that osmoregulation of motility, invasion and capsule expression is mediated through the RcsC/ RcsD/RcsB phosphorelay system. Here we performed gene expression profiling and functional studies to determine the role of TviA, an auxiliary protein of the RcsB response regulator, in controlling virulence gene expression in S. Typhi. TviA repressed expression of genes encoding flagella and the invasionassociated type III secretion system (T3SS-1) through repression of the flagellar regulators flhDC and fliZ, resulting in reduced invasion, reduced motility and reduced expression of FliC. Both RcsB and TviA repressed expression of flhDC, but only TviA altered flhDC expression in response to osmolarity. Introduction of tviA into S. enterica serotype Typhimurium rendered flhDC transcription sensitive to changes in osmolarity. These data suggest that the auxiliary TviA protein integrates a new regulatory input into the RcsB regulon of S. Typhi, thereby altering expression of genes encoding flagella, the Vi antigen and T3SS-1 in response to osmolarity. © 2009 Blackwell Publishing Ltd.
format Journal
author Sebastian E. Winter
Maria G. Winter
Parameth Thiennimitr
Valerie A. Gerriets
Sean Paul Nuccio
Holger Rüssmann
Andreas J. Bäumler
author_facet Sebastian E. Winter
Maria G. Winter
Parameth Thiennimitr
Valerie A. Gerriets
Sean Paul Nuccio
Holger Rüssmann
Andreas J. Bäumler
author_sort Sebastian E. Winter
title The TviA auxiliary protein renders the Satmonella enterica serotype Typhi RcsB regulon responsive to changes in osmolarity
title_short The TviA auxiliary protein renders the Satmonella enterica serotype Typhi RcsB regulon responsive to changes in osmolarity
title_full The TviA auxiliary protein renders the Satmonella enterica serotype Typhi RcsB regulon responsive to changes in osmolarity
title_fullStr The TviA auxiliary protein renders the Satmonella enterica serotype Typhi RcsB regulon responsive to changes in osmolarity
title_full_unstemmed The TviA auxiliary protein renders the Satmonella enterica serotype Typhi RcsB regulon responsive to changes in osmolarity
title_sort tvia auxiliary protein renders the satmonella enterica serotype typhi rcsb regulon responsive to changes in osmolarity
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70350169211&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/48847
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