Role of chitin binding domain of chitinase A of streptomyces cyaneus SP-27 in protoplast formation from schizophyllum commune

• Main Authors: Shigekazu Yano, Arata Honda, Nopakarn Rattanakit-Chandet, Noda Yuta, Mamoru Wakayama, Abhinya Plikomol, Takashi Tachiki
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemistry; Immunology and Microbiology
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=65349158231&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/48884
• Institution: Chiang Mai University

Chitinase A (CHIA) of Streptomyces cyaneus SP-27 forms protoplasts from Schizophyllum commune mycelia when it is combined with α-1,3-glucanase of Bacillus circulans KA-304. An N-terminal chitin-binding domain truncated mutant (CatCHIA), which was expressed in Escherichia coli Rosetta-gami B (DE 3), lost most of its colloidal chitin- and powder chitin-binding activity. The colloidal chitin-hydrolyzing, the powder chitinhydrolyzing, and the protoplast-forming activities of CatCHIA were lower than those of CHIA, suggesting that the chitin-binding domain contributes to the hydrolysis of chitin in the cell-wall of S. commune.