Induction of apoptosis in the human leukemic u937 cell line by kaempferia parviflora wall.ex.baker extract and effects of paclitaxel and camptothecin

Kaempferia parviflora Wall.ex.Baker is a Thai medicinal herb that has high antioxidant and anti-inflammatory activities. Apoptotic effects of the herbal extract alone and in combination with chemotherapeutic drugs, paclitaxel and camptothecin, were here studied in the human promonocytic leukemic U93...

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Main Authors: Ratana Banjerdpongchai, Yupa Chanwikruy, Viboon Rattanapanone, Bungorn Sripanidkulchai
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/48924
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-489242018-08-16T02:16:16Z Induction of apoptosis in the human leukemic u937 cell line by kaempferia parviflora wall.ex.baker extract and effects of paclitaxel and camptothecin Ratana Banjerdpongchai Yupa Chanwikruy Viboon Rattanapanone Bungorn Sripanidkulchai Biochemistry, Genetics and Molecular Biology Medicine Kaempferia parviflora Wall.ex.Baker is a Thai medicinal herb that has high antioxidant and anti-inflammatory activities. Apoptotic effects of the herbal extract alone and in combination with chemotherapeutic drugs, paclitaxel and camptothecin, were here studied in the human promonocytic leukemic U937 cell line. K. parviflora extract suppressed cell proliferation and decreased cell viability in a dose- and time-dependent manner as assessed using the trypan blue exclusion assay. Staining of extract-treated cells with propidium iodide and examination under a fluorescence microscope showed condensed nuclei and apoptotic bodies. Mitochondrial transmembrane potential (MTP) decreased after treatment and the number of cells with decreased MTP also increased. Furthermore, activation of caspase-3 was found in herbal extract-treated cells. When the extract was combined with paclitaxel, an additive effect on U937 cell apoptosis was obtained, whereas camptothecin exerted an antagonistic effect. 2018-08-16T02:06:43Z 2018-08-16T02:06:43Z 2009-01-01 Journal 2476762X 15137368 2-s2.0-77956630914 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77956630914&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/48924
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Ratana Banjerdpongchai
Yupa Chanwikruy
Viboon Rattanapanone
Bungorn Sripanidkulchai
Induction of apoptosis in the human leukemic u937 cell line by kaempferia parviflora wall.ex.baker extract and effects of paclitaxel and camptothecin
description Kaempferia parviflora Wall.ex.Baker is a Thai medicinal herb that has high antioxidant and anti-inflammatory activities. Apoptotic effects of the herbal extract alone and in combination with chemotherapeutic drugs, paclitaxel and camptothecin, were here studied in the human promonocytic leukemic U937 cell line. K. parviflora extract suppressed cell proliferation and decreased cell viability in a dose- and time-dependent manner as assessed using the trypan blue exclusion assay. Staining of extract-treated cells with propidium iodide and examination under a fluorescence microscope showed condensed nuclei and apoptotic bodies. Mitochondrial transmembrane potential (MTP) decreased after treatment and the number of cells with decreased MTP also increased. Furthermore, activation of caspase-3 was found in herbal extract-treated cells. When the extract was combined with paclitaxel, an additive effect on U937 cell apoptosis was obtained, whereas camptothecin exerted an antagonistic effect.
format Journal
author Ratana Banjerdpongchai
Yupa Chanwikruy
Viboon Rattanapanone
Bungorn Sripanidkulchai
author_facet Ratana Banjerdpongchai
Yupa Chanwikruy
Viboon Rattanapanone
Bungorn Sripanidkulchai
author_sort Ratana Banjerdpongchai
title Induction of apoptosis in the human leukemic u937 cell line by kaempferia parviflora wall.ex.baker extract and effects of paclitaxel and camptothecin
title_short Induction of apoptosis in the human leukemic u937 cell line by kaempferia parviflora wall.ex.baker extract and effects of paclitaxel and camptothecin
title_full Induction of apoptosis in the human leukemic u937 cell line by kaempferia parviflora wall.ex.baker extract and effects of paclitaxel and camptothecin
title_fullStr Induction of apoptosis in the human leukemic u937 cell line by kaempferia parviflora wall.ex.baker extract and effects of paclitaxel and camptothecin
title_full_unstemmed Induction of apoptosis in the human leukemic u937 cell line by kaempferia parviflora wall.ex.baker extract and effects of paclitaxel and camptothecin
title_sort induction of apoptosis in the human leukemic u937 cell line by kaempferia parviflora wall.ex.baker extract and effects of paclitaxel and camptothecin
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77956630914&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/48924
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