Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates
An automatic immunoassay system for an assay of sialoglycoconjugates was developed based on the sequential injection technique. A cost effective plain glass capillary tube was used as a solid surface for immobilization of biomolecules via a simple physical adsorption which is adequate to tolerate th...
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th-cmuir.6653943832-489632018-08-16T02:07:19Z Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates Supaporn Kradtap Hartwell Napaporn Wannaprom Prachya Kongtawelert Kate Grudpan Chemistry An automatic immunoassay system for an assay of sialoglycoconjugates was developed based on the sequential injection technique. A cost effective plain glass capillary tube was used as a solid surface for immobilization of biomolecules via a simple physical adsorption which is adequate to tolerate the force of solution flowing through the capillary during the multi-steps immunoassay process. Immunoassay could be performed with many improvements-rapidity per sample as compared to the conventional micro-plate format (40 min vs. 5-8 h); lower cost and simpler as compared to fused silica capillary with covalent immobilization; and without problem of back pressure as compared to flow injection-bead based immunoassay. Performance of the sequential injection-capillary immunoassay was demonstrated by assay of sialoglycoconjugates level in human serum to differentiate cancer patients from healthy people. © 2009. 2018-08-16T02:07:19Z 2018-08-16T02:07:19Z 2009-10-15 Journal 00399140 2-s2.0-67650991817 10.1016/j.talanta.2009.04.020 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67650991817&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/48963 |
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Chemistry Supaporn Kradtap Hartwell Napaporn Wannaprom Prachya Kongtawelert Kate Grudpan Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates |
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An automatic immunoassay system for an assay of sialoglycoconjugates was developed based on the sequential injection technique. A cost effective plain glass capillary tube was used as a solid surface for immobilization of biomolecules via a simple physical adsorption which is adequate to tolerate the force of solution flowing through the capillary during the multi-steps immunoassay process. Immunoassay could be performed with many improvements-rapidity per sample as compared to the conventional micro-plate format (40 min vs. 5-8 h); lower cost and simpler as compared to fused silica capillary with covalent immobilization; and without problem of back pressure as compared to flow injection-bead based immunoassay. Performance of the sequential injection-capillary immunoassay was demonstrated by assay of sialoglycoconjugates level in human serum to differentiate cancer patients from healthy people. © 2009. |
format |
Journal |
author |
Supaporn Kradtap Hartwell Napaporn Wannaprom Prachya Kongtawelert Kate Grudpan |
author_facet |
Supaporn Kradtap Hartwell Napaporn Wannaprom Prachya Kongtawelert Kate Grudpan |
author_sort |
Supaporn Kradtap Hartwell |
title |
Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates |
title_short |
Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates |
title_full |
Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates |
title_fullStr |
Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates |
title_full_unstemmed |
Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates |
title_sort |
sequential injection-capillary immunoassay system for determination of sialoglycoconjugates |
publishDate |
2018 |
url |
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67650991817&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/48963 |
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1681423324667707392 |