A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait

Simple instrumentation and procedure with stopped flow system coupled to hydrodynamic injection and spectrophotometric detection were developed for automatic osmotic fragility test (OFT). OFT is a test for abnormal red blood cells based on the kinetics of their rupturing in a hypotonic saline soluti...

Full description

Saved in:
Bibliographic Details
Main Authors: Supada Khonyoung, Supaporn Kradtap Hartwell, Jaroon Jakmunee, Somchai Lapanantnoppakhun, Torpong Sanguansermsri, Kate Grudpan
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=68249089807&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/48984
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-48984
record_format dspace
spelling th-cmuir.6653943832-489842018-08-16T02:07:41Z A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait Supada Khonyoung Supaporn Kradtap Hartwell Jaroon Jakmunee Somchai Lapanantnoppakhun Torpong Sanguansermsri Kate Grudpan Chemistry Simple instrumentation and procedure with stopped flow system coupled to hydrodynamic injection and spectrophotometric detection were developed for automatic osmotic fragility test (OFT). OFT is a test for abnormal red blood cells based on the kinetics of their rupturing in a hypotonic saline solution. A portion of red cells was merged on-line with a hypotonic saline solution. They were mixed while flowing into the detector and were stopped for a short period for continuously monitoring the change of turbidity based on a transmission signal. In this work, a possible application of the system for screening of beta-thalassemia trait is demonstrated. Descriptions of instrumentation development and parameter optimization are presented. The system offers advantages over the conventional batch-wise OFT in terms of automation, precision, analysis time and sample volume. 2009 © The Japan Society for Analytical Chemistry. 2018-08-16T02:07:41Z 2018-08-16T02:07:41Z 2009-06-01 Journal 13482246 09106340 2-s2.0-68249089807 10.2116/analsci.25.819 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=68249089807&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/48984
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Chemistry
spellingShingle Chemistry
Supada Khonyoung
Supaporn Kradtap Hartwell
Jaroon Jakmunee
Somchai Lapanantnoppakhun
Torpong Sanguansermsri
Kate Grudpan
A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait
description Simple instrumentation and procedure with stopped flow system coupled to hydrodynamic injection and spectrophotometric detection were developed for automatic osmotic fragility test (OFT). OFT is a test for abnormal red blood cells based on the kinetics of their rupturing in a hypotonic saline solution. A portion of red cells was merged on-line with a hypotonic saline solution. They were mixed while flowing into the detector and were stopped for a short period for continuously monitoring the change of turbidity based on a transmission signal. In this work, a possible application of the system for screening of beta-thalassemia trait is demonstrated. Descriptions of instrumentation development and parameter optimization are presented. The system offers advantages over the conventional batch-wise OFT in terms of automation, precision, analysis time and sample volume. 2009 © The Japan Society for Analytical Chemistry.
format Journal
author Supada Khonyoung
Supaporn Kradtap Hartwell
Jaroon Jakmunee
Somchai Lapanantnoppakhun
Torpong Sanguansermsri
Kate Grudpan
author_facet Supada Khonyoung
Supaporn Kradtap Hartwell
Jaroon Jakmunee
Somchai Lapanantnoppakhun
Torpong Sanguansermsri
Kate Grudpan
author_sort Supada Khonyoung
title A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait
title_short A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait
title_full A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait
title_fullStr A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait
title_full_unstemmed A stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: Possibility for automatic screening of beta-thalassemia trait
title_sort stopped flow system with hydrodynamic injection for red blood cells osmotic fragility test: possibility for automatic screening of beta-thalassemia trait
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=68249089807&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/48984
_version_ 1681423328499204096