Production and characterization of anti-dengue capsid antibodies suggesting the N terminus region covering the first 20 amino acids of dengue virus capsid protein is predominantly immunogenic in mice

• Main Authors: Chunya Puttikhunt, Prapapun Ong-ajchaowlerd, Tanapan Prommool, Sutha Sangiambut, Janjuree Netsawang, Thawornchai Limjindaporn, Prida Malasit, Watchara Kasinrerk
• Format: Journal
• Published: 2018
• Subjects: Immunology and Microbiology
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70349560295&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/49136
• Institution: Chiang Mai University

We produced monoclonal and polyclonal antibodies to the capsid (C) protein of dengue serotype 2 virus (DV2 C). First, a maltose-binding protein fused to DV2 C protein (MBP-C) was overproduced in E. coli. The affinity-purified MBP-C protein was cleaved by factor Xa protease to obtain a recombinant DV...