Development of genotype-specific primers for differentiation of genotypes A and B of Aichi viruses

• Main Authors: Ngan Thi Kim Pham, Quang Duy Trinh, Tuan Anh Nguyen, Shuvra Kanti Dey, Tung Gia Phan, Le Phuc Hoang, Pattara Khamrin, Niwat Maneekarn, Shoko Okitsu, Masashi Mizuguchi, Hiroshi Ushijima
• Format: Journal
• Published: 2018
• Subjects: Immunology and Microbiology
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=59749093977&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/49147
• Institution: Chiang Mai University

A nested polymerase chain reaction method using genotype-specific primers based on the capsid gene was developed to differentiate between genotypes A and B of Aichi viruses. Results of the study showed that the PCR using newly designed genotype-specific primers could generate appropriate PCR products from all 17 samples tested, the newly developed primers could differentiate genotype A from genotype B, and all matched those obtained by nucleotide sequencing of the capsid regions. The nested PCR method using genotype-specific primers is useful and can be used for genotyping of Aichi viruses isolated from epidemiological studies. © 2008 Elsevier B.V. All rights reserved.