A new HCV genotype 6 subtype designated 6v was confirmed with three complete genome sequences

Background: Although hepatitis C virus (HCV) genotype 6 is classified into 21 subtypes, 6a-6u, new variants continue to be identified. Objective: To characterize the full-length genomes of three novel HCV genotype 6 variants: KMN02, KM046 and KM181. Study design: From sera of patients with HCV infec...

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Main Authors: Yizhong Wang, Xueshan Xia, Chunhua Li, Niwat Maneekarn, Wenjie Xia, Wenhua Zhao, Yue Feng, Hsiang Fu Kung, Yongshui Fu, Ling Lu
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/49148
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-491482018-08-16T02:15:26Z A new HCV genotype 6 subtype designated 6v was confirmed with three complete genome sequences Yizhong Wang Xueshan Xia Chunhua Li Niwat Maneekarn Wenjie Xia Wenhua Zhao Yue Feng Hsiang Fu Kung Yongshui Fu Ling Lu Immunology and Microbiology Medicine Background: Although hepatitis C virus (HCV) genotype 6 is classified into 21 subtypes, 6a-6u, new variants continue to be identified. Objective: To characterize the full-length genomes of three novel HCV genotype 6 variants: KMN02, KM046 and KM181. Study design: From sera of patients with HCV infection, the entire HCV genome was amplified by RT-PCR followed by direct DNA sequencing and phylogenetic analysis. Results: The sera contained HCV genomes of 9461, 9429, and 9461 nt in length, and each harboured a single ORF of 9051 nt. The genomes showed 95.3-98.1% nucleotide similarity to each other and 72.2-75.4% similarity to 23 genotype 6 reference sequences, which represent subtypes 6a-6u and unassigned variants km41 and gz52557. Phylogenetic analyses demonstrated that they were genotype 6, but were subtypically distinct. Based on the current criteria of HCV classification, they were designed to represent a new subtype, 6v. Analysis of E1 and NS5B region partial sequences revealed two additional related variants, CMBD-14 and CMBD-86 that had been previously reported in northern Thailand and sequences dropped into Genbank. Conclusion: Three novel HCV genotype 6 variants were entirely sequenced and designated subtype 6v. © 2009 Elsevier B.V. All rights reserved. 2018-08-16T02:10:54Z 2018-08-16T02:10:54Z 2009-03-01 Journal 13866532 2-s2.0-60749124401 10.1016/j.jcv.2008.12.009 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=60749124401&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/49148
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
Medicine
spellingShingle Immunology and Microbiology
Medicine
Yizhong Wang
Xueshan Xia
Chunhua Li
Niwat Maneekarn
Wenjie Xia
Wenhua Zhao
Yue Feng
Hsiang Fu Kung
Yongshui Fu
Ling Lu
A new HCV genotype 6 subtype designated 6v was confirmed with three complete genome sequences
description Background: Although hepatitis C virus (HCV) genotype 6 is classified into 21 subtypes, 6a-6u, new variants continue to be identified. Objective: To characterize the full-length genomes of three novel HCV genotype 6 variants: KMN02, KM046 and KM181. Study design: From sera of patients with HCV infection, the entire HCV genome was amplified by RT-PCR followed by direct DNA sequencing and phylogenetic analysis. Results: The sera contained HCV genomes of 9461, 9429, and 9461 nt in length, and each harboured a single ORF of 9051 nt. The genomes showed 95.3-98.1% nucleotide similarity to each other and 72.2-75.4% similarity to 23 genotype 6 reference sequences, which represent subtypes 6a-6u and unassigned variants km41 and gz52557. Phylogenetic analyses demonstrated that they were genotype 6, but were subtypically distinct. Based on the current criteria of HCV classification, they were designed to represent a new subtype, 6v. Analysis of E1 and NS5B region partial sequences revealed two additional related variants, CMBD-14 and CMBD-86 that had been previously reported in northern Thailand and sequences dropped into Genbank. Conclusion: Three novel HCV genotype 6 variants were entirely sequenced and designated subtype 6v. © 2009 Elsevier B.V. All rights reserved.
format Journal
author Yizhong Wang
Xueshan Xia
Chunhua Li
Niwat Maneekarn
Wenjie Xia
Wenhua Zhao
Yue Feng
Hsiang Fu Kung
Yongshui Fu
Ling Lu
author_facet Yizhong Wang
Xueshan Xia
Chunhua Li
Niwat Maneekarn
Wenjie Xia
Wenhua Zhao
Yue Feng
Hsiang Fu Kung
Yongshui Fu
Ling Lu
author_sort Yizhong Wang
title A new HCV genotype 6 subtype designated 6v was confirmed with three complete genome sequences
title_short A new HCV genotype 6 subtype designated 6v was confirmed with three complete genome sequences
title_full A new HCV genotype 6 subtype designated 6v was confirmed with three complete genome sequences
title_fullStr A new HCV genotype 6 subtype designated 6v was confirmed with three complete genome sequences
title_full_unstemmed A new HCV genotype 6 subtype designated 6v was confirmed with three complete genome sequences
title_sort new hcv genotype 6 subtype designated 6v was confirmed with three complete genome sequences
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=60749124401&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/49148
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