Quantitative analysis of oxyresveratrol content in artocarpus lakoocha and 'Puag-Haad'
Objective: To develop a thin-layer chromatography (TLC) densitometric method for the determination of oxyresveratrol content in Artocarpuslakoocha heartwood and in the traditional drug 'Puag-Haad'. Materials and Methods: Sample solution of A. lakoocha heartwood was prepared by Soxhlet extr...
Saved in:
Main Authors: | , , , , , |
---|---|
Format: | Journal |
Published: |
2018
|
Subjects: | |
Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=64349106731&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/49359 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Chiang Mai University |
id |
th-cmuir.6653943832-49359 |
---|---|
record_format |
dspace |
spelling |
th-cmuir.6653943832-493592018-08-16T02:15:13Z Quantitative analysis of oxyresveratrol content in artocarpus lakoocha and 'Puag-Haad' Suthira Maneechai Kittisak Likhitwitayawuid Boonchoo Sritularak Chanida Palanuvej Nijsiri Ruangrungsi Panee Sirisa-Ard Medicine Objective: To develop a thin-layer chromatography (TLC) densitometric method for the determination of oxyresveratrol content in Artocarpuslakoocha heartwood and in the traditional drug 'Puag-Haad'. Materials and Methods: Sample solution of A. lakoocha heartwood was prepared by Soxhlet extraction of the plant material in ethanol, whereas the Puag-Haad solution was obtained by dissolving the drug in methanol. Analysis of each sample solution was performed on a Silica gel 60 F 254 TLC plate (20 × 10 cm) with methylene chloride/methanol (85:15) as the mobile phase. After development, the TLC plate was examined with a TLC scanner in the absorbance mode at 254 nm. The newly developed analytical method was validated using an authentic sample of oxyresveratrol previously isolated from A. lakoocha heartwood, and was used to analyze the oxyresveratrol content in samples of A. lakoocha heartwood and the traditional drug Puag-Haad. Results: A sensitive and reliable TLC densitometric method was successfully developed. The method was validated in terms of accuracy (99.11-102.60%) and precision (1.66-4.23% coefficient of variation). The limits of detection and quantitation were 15.6 and 52 ng/spot, respectively. The amounts of oxyresveratrol in 3 samples of A. lakoocha heartwood collected from its natural habitat were 49.0-182.3 mg/g, whereas those in 11 commercial samples were in the range of 23.4-69.6 mg/g. The oxyresveratrol contents in 2 samples of traditional drug Puag-Haad were 780.1 and 837.5 mg/g. Conclusion: The TLC densitometric method developed in this study is a simple, convenient, sensitive and reliable procedure. It was an effective analytical tool for the evaluation of oxyresveratrol content in both A. lakoocha heartwood and the traditional drug Puag-Haad. Copyright © 2009 S. Karger AG, Basel. 2018-08-16T02:15:13Z 2018-08-16T02:15:13Z 2009-04-01 Journal 10117571 2-s2.0-64349106731 10.1159/000204354 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=64349106731&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/49359 |
institution |
Chiang Mai University |
building |
Chiang Mai University Library |
country |
Thailand |
collection |
CMU Intellectual Repository |
topic |
Medicine |
spellingShingle |
Medicine Suthira Maneechai Kittisak Likhitwitayawuid Boonchoo Sritularak Chanida Palanuvej Nijsiri Ruangrungsi Panee Sirisa-Ard Quantitative analysis of oxyresveratrol content in artocarpus lakoocha and 'Puag-Haad' |
description |
Objective: To develop a thin-layer chromatography (TLC) densitometric method for the determination of oxyresveratrol content in Artocarpuslakoocha heartwood and in the traditional drug 'Puag-Haad'. Materials and Methods: Sample solution of A. lakoocha heartwood was prepared by Soxhlet extraction of the plant material in ethanol, whereas the Puag-Haad solution was obtained by dissolving the drug in methanol. Analysis of each sample solution was performed on a Silica gel 60 F 254 TLC plate (20 × 10 cm) with methylene chloride/methanol (85:15) as the mobile phase. After development, the TLC plate was examined with a TLC scanner in the absorbance mode at 254 nm. The newly developed analytical method was validated using an authentic sample of oxyresveratrol previously isolated from A. lakoocha heartwood, and was used to analyze the oxyresveratrol content in samples of A. lakoocha heartwood and the traditional drug Puag-Haad. Results: A sensitive and reliable TLC densitometric method was successfully developed. The method was validated in terms of accuracy (99.11-102.60%) and precision (1.66-4.23% coefficient of variation). The limits of detection and quantitation were 15.6 and 52 ng/spot, respectively. The amounts of oxyresveratrol in 3 samples of A. lakoocha heartwood collected from its natural habitat were 49.0-182.3 mg/g, whereas those in 11 commercial samples were in the range of 23.4-69.6 mg/g. The oxyresveratrol contents in 2 samples of traditional drug Puag-Haad were 780.1 and 837.5 mg/g. Conclusion: The TLC densitometric method developed in this study is a simple, convenient, sensitive and reliable procedure. It was an effective analytical tool for the evaluation of oxyresveratrol content in both A. lakoocha heartwood and the traditional drug Puag-Haad. Copyright © 2009 S. Karger AG, Basel. |
format |
Journal |
author |
Suthira Maneechai Kittisak Likhitwitayawuid Boonchoo Sritularak Chanida Palanuvej Nijsiri Ruangrungsi Panee Sirisa-Ard |
author_facet |
Suthira Maneechai Kittisak Likhitwitayawuid Boonchoo Sritularak Chanida Palanuvej Nijsiri Ruangrungsi Panee Sirisa-Ard |
author_sort |
Suthira Maneechai |
title |
Quantitative analysis of oxyresveratrol content in artocarpus lakoocha and 'Puag-Haad' |
title_short |
Quantitative analysis of oxyresveratrol content in artocarpus lakoocha and 'Puag-Haad' |
title_full |
Quantitative analysis of oxyresveratrol content in artocarpus lakoocha and 'Puag-Haad' |
title_fullStr |
Quantitative analysis of oxyresveratrol content in artocarpus lakoocha and 'Puag-Haad' |
title_full_unstemmed |
Quantitative analysis of oxyresveratrol content in artocarpus lakoocha and 'Puag-Haad' |
title_sort |
quantitative analysis of oxyresveratrol content in artocarpus lakoocha and 'puag-haad' |
publishDate |
2018 |
url |
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=64349106731&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/49359 |
_version_ |
1681423395769548800 |