Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand

Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of...

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Main Authors: Sorawat Thongsahuan, Visut Baimai, Yasushi Otsuka, Atiporn Saeung, Benjawan Tuetun, Narissara Jariyapan, Sittiporn Suwannamit, Pradya Somboon, Atchariya Jitpakdi, Hiroyuki Takaoka, Wej Choochote
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/49410
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-494102018-08-16T02:16:11Z Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand Sorawat Thongsahuan Visut Baimai Yasushi Otsuka Atiporn Saeung Benjawan Tuetun Narissara Jariyapan Sittiporn Suwannamit Pradya Somboon Atchariya Jitpakdi Hiroyuki Takaoka Wej Choochote Medicine Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2generations, suggest-ing a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies varia-tion (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms. 2018-08-16T02:16:11Z 2018-08-16T02:16:11Z 2009-01-01 Journal 16788060 00740276 2-s2.0-70149093169 10.1590/S0074-02762009000400004 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70149093169&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/49410
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Sorawat Thongsahuan
Visut Baimai
Yasushi Otsuka
Atiporn Saeung
Benjawan Tuetun
Narissara Jariyapan
Sittiporn Suwannamit
Pradya Somboon
Atchariya Jitpakdi
Hiroyuki Takaoka
Wej Choochote
Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
description Seventy-one isolines of Anopheles campestris-like were established from wild-caught females collected from human-biting and animal-biting traps at 12 locations in Thailand. All isolines had an average branch summation of seta 2-VI pupal skins ranging from 20.3-30.0 branches, which is in the range of An. campestris (17-58 branches). They showed three different karyotypes based on the amount of extra heterochromatin in the sex chromosomes, namely Forms B (X2, Y2), E (X1, X2, X3, Y5) and a new karyotypic Form F (X2, X3, Y6). Form B has been found only in Chaing Mai and Kamphaeng Phet populations, while Forms E and F are widely distributed throughout the species range. Genetic crosses between the 12 isolines, which were arbitrarily selected as representatives of An. campestris-like Forms B, E and F, revealed genetic compatibility that provided viable progeny through F2generations, suggest-ing a conspecific nature of these karyotypic forms. These results are supported by the very low intraspecies varia-tion (genetic distance < 0.005) of ITS2, COI and COII from genomic DNA of the three karyotypic forms.
format Journal
author Sorawat Thongsahuan
Visut Baimai
Yasushi Otsuka
Atiporn Saeung
Benjawan Tuetun
Narissara Jariyapan
Sittiporn Suwannamit
Pradya Somboon
Atchariya Jitpakdi
Hiroyuki Takaoka
Wej Choochote
author_facet Sorawat Thongsahuan
Visut Baimai
Yasushi Otsuka
Atiporn Saeung
Benjawan Tuetun
Narissara Jariyapan
Sittiporn Suwannamit
Pradya Somboon
Atchariya Jitpakdi
Hiroyuki Takaoka
Wej Choochote
author_sort Sorawat Thongsahuan
title Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
title_short Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
title_full Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
title_fullStr Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
title_full_unstemmed Karyotypic variation and geographic distribution of Anopheles campestris-like (Diptera: Culicidae) in Thailand
title_sort karyotypic variation and geographic distribution of anopheles campestris-like (diptera: culicidae) in thailand
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70149093169&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/49410
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