Actinomadura apis sp. nov., isolated from a honey bee (Apis mellifera) hive, and the reclassification of Actinomadura cremea subsp. rifamycini Gauze et al. 1987 as Actinomadura rifamycini (Gauze et al.1987) sp. nov., comb. nov

A Gram-reaction-positive aerobic actinomycete, designated strain IM17-1 T, was isolated from a honey bee (Apis mellifera) hive in Chiang Mai Province, Thailand. The strain formed a branched substrate mycelium and mature aerial mycelium bore short chains of arthrospores with warty surfaces. The cell...

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Main Authors: Yaowanoot Promnuan, Takuji Kudo, Moriya Ohkuma, Panuwan Chantawannakul
Format: Journal
Published: 2018
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spelling th-cmuir.6653943832-495682018-09-04T04:13:25Z Actinomadura apis sp. nov., isolated from a honey bee (Apis mellifera) hive, and the reclassification of Actinomadura cremea subsp. rifamycini Gauze et al. 1987 as Actinomadura rifamycini (Gauze et al.1987) sp. nov., comb. nov Yaowanoot Promnuan Takuji Kudo Moriya Ohkuma Panuwan Chantawannakul Agricultural and Biological Sciences Immunology and Microbiology A Gram-reaction-positive aerobic actinomycete, designated strain IM17-1 T, was isolated from a honey bee (Apis mellifera) hive in Chiang Mai Province, Thailand. The strain formed a branched substrate mycelium and mature aerial mycelium bore short chains of arthrospores with warty surfaces. The cell wall contained meso-2,6-diaminopimelic acid (cell-wall type III) and the whole cell sugars were fucose, galactose, glucose, madurose, mannose and ribose. The major isoprenoid quinone was hexahydrogenated menaquinone with nine isoprene units and the predominant cellular fatty acids were C 16: 0 (33.8%), C 18: 1ω9c (32.7%), summed feature 3 (C 16: 1ω7c and/or iso-C 15: 0 2-OH) (8.7%) and 10-methyl C 18: 0 (8.2%). The phospholipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. These morphological and chemotaxonomic characteristics were consistent with the classification of IM17-1 T within the genus Actinomadura. Based on 16S rRNA gene sequence analysis, strain IM17-1 T was closely related to the type strains of Actinomadura cremea subsp. cremea (98.1%) and Actinomadura cremea subsp. rifamycini (98.6%); however, it represented a distinct phylogenetic lineage from the other species within this genus. The unique genetic characteristics were reaffirmed by low levels of DNA-DNA relatedness between strain IM17-1 T and the two most closely related type strains, A. cremea subsp. cremea JCM 3308T (56.5±4.9%) and A. cremea subsp. rifamycini JCM 3309T (31.0±22.6%), and further supported the proposal of IM17-1 T as a novel species. Strain IM17-1T (5JCM 16576T 5TISTR 1980T) thus represents a novel species of the genus Actinomadura, for which the name Actinomadura apis sp. nov. is proposed. In addition, the genotypic and phenotypic data suggested the reclassification of Actinomadura cremea subsp. rifamycini Gauze et al. 1987 as a separate species, Actinomadura rifamycini sp. nov., comb. nov. © 2011 IUMS. 2018-09-04T04:04:05Z 2018-09-04T04:04:05Z 2011-09-01 Journal 14665026 2-s2.0-80054716872 10.1099/ijs.0.026633-0 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80054716872&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/49568
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Immunology and Microbiology
spellingShingle Agricultural and Biological Sciences
Immunology and Microbiology
Yaowanoot Promnuan
Takuji Kudo
Moriya Ohkuma
Panuwan Chantawannakul
Actinomadura apis sp. nov., isolated from a honey bee (Apis mellifera) hive, and the reclassification of Actinomadura cremea subsp. rifamycini Gauze et al. 1987 as Actinomadura rifamycini (Gauze et al.1987) sp. nov., comb. nov
description A Gram-reaction-positive aerobic actinomycete, designated strain IM17-1 T, was isolated from a honey bee (Apis mellifera) hive in Chiang Mai Province, Thailand. The strain formed a branched substrate mycelium and mature aerial mycelium bore short chains of arthrospores with warty surfaces. The cell wall contained meso-2,6-diaminopimelic acid (cell-wall type III) and the whole cell sugars were fucose, galactose, glucose, madurose, mannose and ribose. The major isoprenoid quinone was hexahydrogenated menaquinone with nine isoprene units and the predominant cellular fatty acids were C 16: 0 (33.8%), C 18: 1ω9c (32.7%), summed feature 3 (C 16: 1ω7c and/or iso-C 15: 0 2-OH) (8.7%) and 10-methyl C 18: 0 (8.2%). The phospholipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. These morphological and chemotaxonomic characteristics were consistent with the classification of IM17-1 T within the genus Actinomadura. Based on 16S rRNA gene sequence analysis, strain IM17-1 T was closely related to the type strains of Actinomadura cremea subsp. cremea (98.1%) and Actinomadura cremea subsp. rifamycini (98.6%); however, it represented a distinct phylogenetic lineage from the other species within this genus. The unique genetic characteristics were reaffirmed by low levels of DNA-DNA relatedness between strain IM17-1 T and the two most closely related type strains, A. cremea subsp. cremea JCM 3308T (56.5±4.9%) and A. cremea subsp. rifamycini JCM 3309T (31.0±22.6%), and further supported the proposal of IM17-1 T as a novel species. Strain IM17-1T (5JCM 16576T 5TISTR 1980T) thus represents a novel species of the genus Actinomadura, for which the name Actinomadura apis sp. nov. is proposed. In addition, the genotypic and phenotypic data suggested the reclassification of Actinomadura cremea subsp. rifamycini Gauze et al. 1987 as a separate species, Actinomadura rifamycini sp. nov., comb. nov. © 2011 IUMS.
format Journal
author Yaowanoot Promnuan
Takuji Kudo
Moriya Ohkuma
Panuwan Chantawannakul
author_facet Yaowanoot Promnuan
Takuji Kudo
Moriya Ohkuma
Panuwan Chantawannakul
author_sort Yaowanoot Promnuan
title Actinomadura apis sp. nov., isolated from a honey bee (Apis mellifera) hive, and the reclassification of Actinomadura cremea subsp. rifamycini Gauze et al. 1987 as Actinomadura rifamycini (Gauze et al.1987) sp. nov., comb. nov
title_short Actinomadura apis sp. nov., isolated from a honey bee (Apis mellifera) hive, and the reclassification of Actinomadura cremea subsp. rifamycini Gauze et al. 1987 as Actinomadura rifamycini (Gauze et al.1987) sp. nov., comb. nov
title_full Actinomadura apis sp. nov., isolated from a honey bee (Apis mellifera) hive, and the reclassification of Actinomadura cremea subsp. rifamycini Gauze et al. 1987 as Actinomadura rifamycini (Gauze et al.1987) sp. nov., comb. nov
title_fullStr Actinomadura apis sp. nov., isolated from a honey bee (Apis mellifera) hive, and the reclassification of Actinomadura cremea subsp. rifamycini Gauze et al. 1987 as Actinomadura rifamycini (Gauze et al.1987) sp. nov., comb. nov
title_full_unstemmed Actinomadura apis sp. nov., isolated from a honey bee (Apis mellifera) hive, and the reclassification of Actinomadura cremea subsp. rifamycini Gauze et al. 1987 as Actinomadura rifamycini (Gauze et al.1987) sp. nov., comb. nov
title_sort actinomadura apis sp. nov., isolated from a honey bee (apis mellifera) hive, and the reclassification of actinomadura cremea subsp. rifamycini gauze et al. 1987 as actinomadura rifamycini (gauze et al.1987) sp. nov., comb. nov
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=80054716872&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/49568
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