Detection and genetic characterization of rotavirus infections in non-hospitalized children with acute gastroenteritis in Japan, 2007-2009

The molecular epidemiology of rotavirus infections in non-hospitalized children in five different regions (Sapporo, Saga, Tokyo, Osaka, and Maizuru) of Japan during 2007-2009 was investigated. Overall, rotavirus was detected in 156 out of 1008 (15.5%) specimens. The rotavirus infection in 2007-2008...

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Main Authors: Wisoot Chan-It, Aksara Thongprachum, Shuvra Kanti Dey, Tung Gia Phan, Pattara Khamrin, Shoko Okitsu, Shuichi Nishimura, Masaaki Kobayashi, Hideaki Kikuta, Tsuneyoshi Baba, Atsuko Yamamoto, Kumiko Sugita, Shintaro Hashira, Takeshi Tajima, Shinichi Ishida, Masashi Mizuguchi, Hiroshi Ushijima
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/49621
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spelling th-cmuir.6653943832-496212018-09-04T04:27:28Z Detection and genetic characterization of rotavirus infections in non-hospitalized children with acute gastroenteritis in Japan, 2007-2009 Wisoot Chan-It Aksara Thongprachum Shuvra Kanti Dey Tung Gia Phan Pattara Khamrin Shoko Okitsu Shuichi Nishimura Masaaki Kobayashi Hideaki Kikuta Tsuneyoshi Baba Atsuko Yamamoto Kumiko Sugita Shintaro Hashira Takeshi Tajima Shinichi Ishida Masashi Mizuguchi Hiroshi Ushijima Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine The molecular epidemiology of rotavirus infections in non-hospitalized children in five different regions (Sapporo, Saga, Tokyo, Osaka, and Maizuru) of Japan during 2007-2009 was investigated. Overall, rotavirus was detected in 156 out of 1008 (15.5%) specimens. The rotavirus infection in 2007-2008 (19.3%) was higher than those in 2008-2009 (12.1%). G1P[8] was the most prevalent (62.8%), followed by G3P[8] (21.8%), G9P[8] (14.7%), and G2P[4] (0.7%). Interestingly, the number of G3P[8] strains increased threefold from the former season (2006-2007) from 7.3% to 21.8%, whereas G2P[4] and G9P[8] decreased from 11.4% to 0.7% and 20.3% to 14.7%, respectively. In the phylogenetic analysis, G3 rotaviruses were closely related to " the new variant G3" 5091 strain, which previously emerged in Japan and China. G9 viruses isolated in 2007-2008 were genetically close to the Thai strain, while those isolated in 2008-2009 had a close relationship with Chinese strains. G1 viruses appeared to be more similar to the recently reported G1 strain in China. Nucleotide sequence analysis of 33 P[8]-nontypeable strains revealed 5 nucleotide mismatches at the primer binding site. Based on previously reported (2003-2007) and current (2007-2009) data of rotavirus surveillance in the five areas of Japan, it was revealed that in Sapporo, Osaka, and Maizuru, G1P[8] and G3P[8] were detected at high frequencies, ranging from 47.2 to 57.7% and 31.7 to 47.4%, respectively. In Tokyo, G1P[8] (47.4%) was the predominant strain, followed by G9P[8] (20.6%), whereas in Saga, G3P[8] (38.9%) and G9P[8] (36.1%) were identified as the most dominant types. None of G9P[8] was detected in Sapporo. This study highlights the genetic diversity and the significance of rotavirus diarrhea in Japan. © 2010 Elsevier B.V. 2018-09-04T04:04:36Z 2018-09-04T04:04:36Z 2011-03-01 Journal 15671348 2-s2.0-79951948128 10.1016/j.meegid.2010.11.018 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79951948128&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/49621
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
spellingShingle Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
Wisoot Chan-It
Aksara Thongprachum
Shuvra Kanti Dey
Tung Gia Phan
Pattara Khamrin
Shoko Okitsu
Shuichi Nishimura
Masaaki Kobayashi
Hideaki Kikuta
Tsuneyoshi Baba
Atsuko Yamamoto
Kumiko Sugita
Shintaro Hashira
Takeshi Tajima
Shinichi Ishida
Masashi Mizuguchi
Hiroshi Ushijima
Detection and genetic characterization of rotavirus infections in non-hospitalized children with acute gastroenteritis in Japan, 2007-2009
description The molecular epidemiology of rotavirus infections in non-hospitalized children in five different regions (Sapporo, Saga, Tokyo, Osaka, and Maizuru) of Japan during 2007-2009 was investigated. Overall, rotavirus was detected in 156 out of 1008 (15.5%) specimens. The rotavirus infection in 2007-2008 (19.3%) was higher than those in 2008-2009 (12.1%). G1P[8] was the most prevalent (62.8%), followed by G3P[8] (21.8%), G9P[8] (14.7%), and G2P[4] (0.7%). Interestingly, the number of G3P[8] strains increased threefold from the former season (2006-2007) from 7.3% to 21.8%, whereas G2P[4] and G9P[8] decreased from 11.4% to 0.7% and 20.3% to 14.7%, respectively. In the phylogenetic analysis, G3 rotaviruses were closely related to " the new variant G3" 5091 strain, which previously emerged in Japan and China. G9 viruses isolated in 2007-2008 were genetically close to the Thai strain, while those isolated in 2008-2009 had a close relationship with Chinese strains. G1 viruses appeared to be more similar to the recently reported G1 strain in China. Nucleotide sequence analysis of 33 P[8]-nontypeable strains revealed 5 nucleotide mismatches at the primer binding site. Based on previously reported (2003-2007) and current (2007-2009) data of rotavirus surveillance in the five areas of Japan, it was revealed that in Sapporo, Osaka, and Maizuru, G1P[8] and G3P[8] were detected at high frequencies, ranging from 47.2 to 57.7% and 31.7 to 47.4%, respectively. In Tokyo, G1P[8] (47.4%) was the predominant strain, followed by G9P[8] (20.6%), whereas in Saga, G3P[8] (38.9%) and G9P[8] (36.1%) were identified as the most dominant types. None of G9P[8] was detected in Sapporo. This study highlights the genetic diversity and the significance of rotavirus diarrhea in Japan. © 2010 Elsevier B.V.
format Journal
author Wisoot Chan-It
Aksara Thongprachum
Shuvra Kanti Dey
Tung Gia Phan
Pattara Khamrin
Shoko Okitsu
Shuichi Nishimura
Masaaki Kobayashi
Hideaki Kikuta
Tsuneyoshi Baba
Atsuko Yamamoto
Kumiko Sugita
Shintaro Hashira
Takeshi Tajima
Shinichi Ishida
Masashi Mizuguchi
Hiroshi Ushijima
author_facet Wisoot Chan-It
Aksara Thongprachum
Shuvra Kanti Dey
Tung Gia Phan
Pattara Khamrin
Shoko Okitsu
Shuichi Nishimura
Masaaki Kobayashi
Hideaki Kikuta
Tsuneyoshi Baba
Atsuko Yamamoto
Kumiko Sugita
Shintaro Hashira
Takeshi Tajima
Shinichi Ishida
Masashi Mizuguchi
Hiroshi Ushijima
author_sort Wisoot Chan-It
title Detection and genetic characterization of rotavirus infections in non-hospitalized children with acute gastroenteritis in Japan, 2007-2009
title_short Detection and genetic characterization of rotavirus infections in non-hospitalized children with acute gastroenteritis in Japan, 2007-2009
title_full Detection and genetic characterization of rotavirus infections in non-hospitalized children with acute gastroenteritis in Japan, 2007-2009
title_fullStr Detection and genetic characterization of rotavirus infections in non-hospitalized children with acute gastroenteritis in Japan, 2007-2009
title_full_unstemmed Detection and genetic characterization of rotavirus infections in non-hospitalized children with acute gastroenteritis in Japan, 2007-2009
title_sort detection and genetic characterization of rotavirus infections in non-hospitalized children with acute gastroenteritis in japan, 2007-2009
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79951948128&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/49621
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