Detection of α-thalassemia-1 Southeast Asian and Thai type deletions and β-thalassemia 3.5-kb deletion by single-tube multiplex real-time PCR with SYBR green 1 and high-resolution melting analysis

• Main Authors: Sakorn Pornprasert, Thanatcha Wiengkum, Sarinee Srithep, Isarapong Chainoi, Panthong Singboottra, Sanchai Wongwiwatthananukit
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Medicine
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79961076404&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/49704
• Institution: Chiang Mai University

Background: Prevention and control of thalassemia requires simple, rapid, and accurate screening tests for carrier couples who are at risk of conceiving fetuses with severe thalassemia. Methods: Single-tube multiplex real-time PCR with SYBR Green1 and high-resolution melting (HRM) analysis were used...